Enzyme Dynamics and Regulation

Front Matter....Pages i-xxi
Mechanistic Studies on DNA Polymerase I....Pages 1-5
Assembly and Catalytic Functions of the Subunits of Succinyl Coenzyme A Synthetase....Pages 6-16
Fatty Acid Synthetase of Chicken Liver: A Novel Active-Site Structure for Condensation Comprised of SH Groups from a Cysteine Residue and an Oscillating Phosphopantetheine Swinging Arm on Adjacent Subunits....Pages 17-27
Regulatory Properties of Glucokinase....Pages 28-39
Mechanism of Activation of Calmodulin-Dependent Phosphatase by Divalent Metal Ions....Pages 40-47
Studies on the Mechanism and Molecular Mode of Regulation of Fructose-1,6-bisphosphatase....Pages 48-54
Phosphorylation and dAMP Inhibition of Snake Muscle Fructose-1,6-bisphosphatase....Pages 55-61
Identification and Characterization of Intermediates in the Mechanism of Enzyme Action....Pages 62-70
Conformational Dynamics in RNA—Protein Interactions: Immobilization of the Functional Domains in tRNA fMet and Methionyl-tRNA Synthetase....Pages 71-76
Structural and Kinetic Studies of Escherichia coli Glutamine Synthetase....Pages 77-83
Cu,Zn Superoxide Dismutase: A Case of Metalloenzyme Catalysis in Which the Protein Moiety Plays a Major Role....Pages 84-91
Dynamic Participation of Protein Domains in Catalysis by 2-Oxo Acid Dehydrogenase Multienzyme Complexes....Pages 92-101
Membrane-Bound GTP-Transducin Efficiently Activates Retinal cGMP Phosphodiesterase....Pages 102-112
Signal Transduction in the β -Adrenoceptor-Dependent Adenylate Cyclase....Pages 113-120
Acetyl-CoA Carboxylase: Correlation of Phosphorylation State with Allosteric Properties and Physiological State....Pages 121-127
Role of Cyclic Cascades in Metabolic Regulation....Pages 128-135
Regulation of Glutamine Synthetase Activity and Its Biosynthesis in Escherichia coli : Mediation by Three Cycles of Covalent Modification....Pages 136-145
Regulation of ATP, Mg-Dependent Protein Phosphatases....Pages 146-154
Molecular Mechanisms of Allosteric Regulation in Aspartate Transcarbamylase....Pages 155-161
Dynamic Interactions of the Second Messenger Systems....Pages 162-174
Role of Multienzyme Complexes in the Integration of Cellular Metabolism....Pages 175-180
Dynamics of Deoxynucleotide Synthesis in Relation to DNA Replication....Pages 181-189
Differential Stabilization of Left-Handed Z-DNA and Z-RNA In Vitro and In Vivo....Pages 190-199
Interaction of Restriction Endonucleases with Phosphorothioate DNA....Pages 200-205
Role of Conformational Dynamics of Myosin in Muscle Contraction....Pages 206-216
Enzymatic Modulation of Cytoskeletal Self-Assembly: ADP Ribosylation of Microtubule Protein Components....Pages 217-223
Human Angiogenin: An Organogenic Protein....Pages 224-225
Structure and Mechanism of Action of a Membrane-Bound Enzyme: Chloroplast Coupling Factor....Pages 226-234
Electrostatic Effects and the Dynamics of Multienzyme Reactions at the Surface of Plant Cells....Pages 235-246
Energy Transduction by Electroconformational Coupling....Pages 247-260
Topics in Petroleum Biotechnology....Pages 261-267
Experimental Determination of Rate Constants in Enzymatic Reactions....Pages 268-274
Applications of Alternative Substrate Kinetics: In Vivo and In Vitro Data on Aminoglycoside Antibiotic Inactivating Enzymes....Pages 275-288
Kinetics of Irreversible Modification of Enzyme Activity....Pages 289-300
Applications of Stable Isotopes in Biochemistry....Pages 301-311
Enzyme Dynamics in Nonaqueous Media at Subzero Temperatures....Pages 312-321
Kinetic—Structural Organization of Enzyme Systems....Pages 322-326
Relation Between Structure and Function in cAMP-Dependent Protein Kinases....Pages 327-341
Conformation and Dynamics of Oligomeric Enzymes....Pages 342-350
Coenzyme Binding Site of NADP-Dependent Isocitrate Dehydrogenase....Pages 351-361
Significance of Domain Structure of Calmodulin on the Activation of Ca 2+ —Calmodulin-Requiring Enzymes....Pages 362-366
Modification of Leucyl-tRNA Synthetase by Affinity Labeling and Limited Proteolysis....Pages 367-376
NAD Metabolism in Eukaryotic Cells: Purification and Characterization of NMN Adenylyltransferase from Baker’s Yeast....Pages 377-384
Specific β - d -Fucosidase from Aspergillus phoenicis ....Pages 385-393
Formation of Active Aspartate Transcarbamoylase from Defective Polypeptide Chains Produced by Site-Directed Mutagenesis....Pages 394-404
Catalytic Mechanisms Revealed by Protein Engineering of Tyrosyl-tRNA Synthetase....Pages 405-410
Site-Directed Mutagenesis of Alkaline Phosphatase....Pages 411-414
Back Matter....Pages 415-421