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ویرایش: 1 نویسندگان: Gary K. Ackers, Michael L. Johnson (Eds.) سری: Methods in Enzymology 295 ISBN (شابک) : 9780121821968, 0076687929 ناشر: Academic Press سال نشر: 1998 تعداد صفحات: 583 زبان: English فرمت فایل : PDF (درصورت درخواست کاربر به PDF، EPUB یا AZW3 تبدیل می شود) حجم فایل: 13 مگابایت
در صورت تبدیل فایل کتاب Energetics of Biological Macromolecules Part B به فرمت های PDF، EPUB، AZW3، MOBI و یا DJVU می توانید به پشتیبان اطلاع دهید تا فایل مورد نظر را تبدیل نمایند.
توجه داشته باشید کتاب انرژی ماکرومولکول های بیولوژیکی قسمت B نسخه زبان اصلی می باشد و کتاب ترجمه شده به فارسی نمی باشد. وبسایت اینترنشنال لایبرری ارائه دهنده کتاب های زبان اصلی می باشد و هیچ گونه کتاب ترجمه شده یا نوشته شده به فارسی را ارائه نمی دهد.
توضیحات کلی حجم:
وجود ساختارهای بیولوژیکی و تعاملات عملکردی آنها توسط روابط
پرانرژی دیکته می شود. بنابراین موضوع اصلی این جلد این است که
روشهای ترمودینامیکی، یعنی تکنیکهایی که انرژی ماکرومولکولهای
بیولوژیکی را بررسی میکنند، اکنون یک خانواده قدرتمند و کاربردی
از ابزارها برای تحقیق در زیستشناسی مدرن را تشکیل میدهند.
کاربرد ترمودینامیک و ترمودینامیک آماری در سیستم های بیوشیمیایی
و بیوفیزیکی ارائه شده است. این جلد مکمل روشها در
آنزیمولوژی، جلد 259 است.
توضیحات کلی مجموعه:
استاندارد آزمایشگاهی مورد تحسین منتقدان برای بیش از چهل سال،
Methods in Enzymologyیکی از معتبرترین نشریات در زمینه
بیوشیمی است. از سال 1955، هر جلد مشتاقانه مورد انتظار، مکرراً
مورد مشورت و تمجید محققان و داوران قرار گرفته است. اکنون با بیش
از 300 جلد (همه آنها هنوز در دست چاپ هستند)، این مجموعه حاوی
مطالب زیادی است که امروزه هنوز مرتبط است - واقعاً یک نشریه
ضروری برای محققان در همه زمینه های علوم زیستی.
ویژگی های کلیدی
* موضوعات اصلی تحت پوشش عبارتند از:
* رمزگشایی قوانین پایداری مارپیچ در پپتیدها
* تاخوردگی پروتئین در غشاء
* ازدحام مولکولی
* مطالعه بور اثر در هموگلوبین حدواسط
* کالریمتری فوتوآکوستیک پروتئینها
* جنبه های نظری کالریمتری تیتراسیون همدما
* روشهای پرانرژی برای مطالعه رپرسور بیوتین دو کاره
General Description of the Volume:
The very existence of biological structures and their
functional interactions are dictated by energetic
relationships. Thus the central theme of this volume is that
thermodynamic methods, i.e. techniques that probe the
energetics of biological macromolecules, now comprise a
powerful and practical family of tools for research in modern
biology. The application of thermodynamics and statistical
thermodynamics to biochemical and biophysical systems is
presented. This volume supplements Methods in Enzymology,
Volume 259.
General Description of the Series:
The critically acclaimed laboratory standard for more than
forty years, Methods in Enzymology is one of the most
highly respected publications in the field of biochemistry.
Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now
with more than 300 volumes (all of them still in print), the
series contains much material still relevant today--truly an
essential publication for researchers in all fields of life
sciences.
Key Features
* Major topics covered include:
* Deciphering rules of helix stability in peptides
* Protein Folding in Membranes
* Molecular Crowding
* Study of the Bohr Effect in Hemoglobin Intermediates
* Photoacoustic Calorimetry of Proteins
* Theoretical Aspects of Isothermal Titration Calorimetry
* Energetic Methods to Study Bifunctional Biotin Repressor
Content:
Contributors to volume xxix
Pages xi-xv
Preface
Page xvii
Lawrence Grossman, Kivie Moldave
Volumes in series
Pages xix-xxi
[1] DNA polymerase I from Escherichia coli Original Research Article
Pages 3-12
Peter Setlow
[2] The isolation and properties of DNA polymerase II from Escherichia coli Original Research Article
Pages 13-22
Robb E. Moses
[3] Deoxyribonucleic acid polymerase III (Escherichia coli K12) Original Research Article
Pages 22-26
Thomas Kornberg, Malcolm L. Gefter
[4] Bacillus subtilis DNA polymerases Original Research Article
Pages 27-38
Kenneth B. Gass, Nicholas R. Cozzarelli
[5] The purification of the DNA polymerase from Micrococcus luteus Original Research Article
Pages 38-45
Lester D.G. Hamilton
[6] T4 DNA polymerasel Original Research Article
Pages 46-53
I.R. Lehman
[7] Sea urchin nuclear DNA polymerasel Original Research Article
Pages 53-70
Bradford S. Fansler, Lawrence A. Loeb
[8] Nucleotide polymerizing enzymes from calf thymus gland Original Research Article
Pages 70-81
F.J. Bollum, Lucy M.S. Chang, C.M. Tsiapalis, J.W. Dorson
[9] Purification and properties of low molecular weight DNA polymerase from mammalian cells Original Research Article
Pages 81-88
Lucy M.S. Chang
[10] The DNA polymerases of KB cells Original Research Article
Pages 89-102
W. David Sedwick, Teresa Shu-Fong Wang, David Korn
[11] Synthesis of [α-32P]ribo- and deoxyribonucleoside 5′-triphosphates Original Research Article
Pages 102-115
R.H. Symons
[12] Endogenous RNA-directed DNA polymerase activity in virions of RNA tumor viruses and in a fraction from normal chicken cells Original Research Article
Pages 119-124
Satoshi Mizutani, Chil-Yong Kang, Howard M. Temin
[13] Purification of the RNA-directed DNA polymerase from avian myeloblastosis virus and its assay with polynucleotide templates Original Research Article
Pages 125-130
Inder M. Verma, David Baltimore
[14] Identification of viral reverse-transcriptase Original Research Article
Pages 130-143
Edward M. Scolnick, Wade P. Parks
[15] RNA-dependent DNA polymerase from avian myeloblastosis virus Original Research Article
Pages 143-150
J. Leis, J. Hurwitz
[15a] Purification and detection of reverse transcriptase in viruses and cells Original Research Article
Pages 150-173
D.L. Kacian, S. Spiegelman
[15b] The preparation of RNA-directed DNA polymerase from ovaries of Xenopus laevis Original Research Article
Pages 173-177
Ronald D. Brown, Glauco P. Tocchini-Valentini
[16] Mass screening for mutants with altered DNases by microassay techniques Original Research Article
Pages 180-193
Bernard Weiss, Christine Milcarek
[17] Protein (П‰) from Escherichia coli Original Research Article
Pages 197-203
James C. Wang
[18] Purification of TF1—A template-specific DNA-binding protein and transcription inhibitor from bacteriophage SPO1-Infected Bacillus subtilis Original Research Article
Pages 204-215
G.G. Johnson, E.P. Geiduschek
[19] DNA synthesis in toluene-treated cells of Escherichia coli Original Research Article
Pages 219-224
Robs E. Moses
[20] DNA Replication in Plasmolysed Escherichia coli Original Research Article
Pages 224-227
Reed B. Wickner
[21] Nucleotide sequence analysis of bacteriophage DNA Original Research Article
Pages 231-253
Ray Wu, R. Padmanabhan, Robert Bambara
[22] Preparation of RNA transcripts of discrete segments of DNA Original Research Article
Pages 254-272
Charles A. Marotta, Paul Lebowitz, Ravi Dhar, B.Sayeeda Zain, Sherman M. Weissman
[23] The use of the T4 DNA polymerase in identification of 3′ terminal nucleotide sequences of duplex DNA Original Research Article
Pages 273-281
P.T. Englund, S.S. Price, P.H. Weigel
[24] Enzymatic methods for sequence analysis applied to DNA restriction and methylation sites Original Research Article
Pages 282-294
H.O. Smith, T.J. Kelly, P.H. Roy
[25] The use of ribo-substitution techniques for determining DNA sequences Original Research Article
Pages 295-321
Philip Whitcodie, Kirk Fry, Winston Salser
[26] 3′ End-group labeling and partial sequence determination of oligodeoxynucleotides Original Research Article
Pages 322-341
Hans Kossel, Ranajit Roychoudhury, Dietrich Fischer, Angela Otto
[27] A new approach to the study of nucleotide sequences in DNA: the analysis of termini formed by DNases Original Research Article
Pages 341-355
Giorgio Bernardi, Stanislav D. Ehrlich, Jean-Paul Thiery
[28] Analysis of labeled 3′ terminal nucleotides of DNA fragments Original Research Article
Pages 355-359
Umberto Bertazzoni, Stanislav D. Ehrlich, Giorgio Bernardi
[29] Analysis of repeating DNA sequences by reassociation Review Article
Pages 363-418
Roy J. Britten, Dale E. Graham, Berney R. Neufeld
[29a] Isolation of transfer ribonucleic acid cistrons and 5 S ribonucleic acid cistrons from Escherichia coli Original Research Article
Pages 419-431
B.P. Doctor, Don J. Brenner, W.L. Miller
[30] Purification of messenger RNA and heterogeneous nuclear RNA containing poly(A) sequences Original Research Article
Pages 431-443
Hiroshi Nakazato, Mary Edmonds
[31] Formation and visualization of rings from eukaryotic DNA fragments Original Research Article
Pages 443-451
C.S. Lee, C.A. Thomas Jr.
[32] Denaturation mapping of DNA Original Research Article
Pages 451-458
Ross B. Inman
[33] Identification of repeating units in DNA concatemers Original Research Article
Pages 459-466
A. Skalka, P. Bartl
[34] Improved separation of oligonucleotides by reversed-phase chromatography Original Research Article
Pages 469-472
B.Z. Egan, A.D. Kelmers
[35] Preparation of transfer RNA fragments by limited degradation with spleen exonuclease Original Research Article
Pages 473-477
P. Philippsen, H.G. Zachau
[36] The production of limit-oligonucleotide digests from phenylalanine tRNA (Escherichia coli) with snake venom phosphodiesterase Original Research Article
Pages 477-482
M.P. Stulberg, K.R. Isham
[37] Miniature reversed-phase chromatography systems for the rapid resolution of transfer RNA\'s and Ribosomal RNA\'s Original Research Article
Pages 483-486
A.D. Kelmers, D.E. Heatherly, B.Z. Egan
[38] The qualitative analysis and preparation of phage transfer RNA Original Research Article
Pages 487-491
Neal Scherberg, Samuel B. Weiss
[39] Isolation, purification, and methylation of undermethylated tRNAPhe from an RCrel mutant of Escherichia coli Original Research Article
Pages 492-502
Lee Shugart, M.P. Stulberg
[40] Preparation of tRNA\'s and aminoacyl-tRNA synthetases from Bacillus subtilis cells at various stages of growth and spores Original Research Article
Pages 502-510
Barbara S. Vold
[41] Preparation of crude tRNA and aminoacyl-tRNA synthetase from calf liver Original Research Article
Pages 510-514
C.W. Rancher, R.L. Pearson, A.D. Kelmers
[42] Purification of calf liver tRNA\'s Original Research Article
Pages 514-522
R.L. Pearson, J.F. Weiss, D.W. Holladay, A.D. Kelmers
[43] Fractionation of rabbit liver methionyl-tRNA species Original Research Article
Pages 522-529
Kallol K. Bose, Nando K. Chatterjee, Naba K. Gupta
[44] Specific aminoacylation of the methionine-apecific tRNA\'s of eukaryotes Original Research Article
Pages 530-547
Wendell M. Stanley Jr.
[45] Purification of tyrosine: tRNA ligase, valine : tRNA ligase, alanine : tRNA ligase, and isoleucine : tRNA ligase from Saccharomyces cerevisiae О±S288C Original Research Article
Pages 547-576
Opinder S. Bhanot, Željko Kućan, Shohei Aoyagi, Florence C. Lee, Robert W. Chambers
[46] Aminoacyl-tRNA synthetase complex from rat liver Original Research Article
Pages 577-583
Murray P. Deutscher
[47] Particle character of aminoacyl-tRNA synthetases isolated from a postmicrosomal fraction Original Research Article
Pages 584-600
Claudia Vennegoor, Hans Bloemendal
[48] Kinetic techniques for the investigation of amino acid: tRNA ligases (aminoacyl-tRNA synthetases, amino acid activating enzymes) Original Research Article
Pages 601-619
Elizabeth Ann Eigner, Robert B. Loftfield
[49] Kinetics of aminoacyl-tRNA synthetases catalyzed ATP-PPi exchange Original Research Article
Pages 620-627
Daniel V. Santi, Robert W. Webster Jr., W.W. Cleland
[50] Applications of kinetic methods to aminoacyl-tRNA synthetases Original Research Article
Pages 627-642
Christian F. Midelfort, Alan H. Mehler
[51] Investigation of recognition sites in valine tRNA I (Baker\'s yeast) by dissected molecule method Original Research Article
Pages 643-661
A.D. Mirzabekov, A.A. Bayev
[52] Assay for AA-tRNA recognition by the EFTu-GTP complex of Escherichia coli Original Research Article
Pages 661-667
Tames Ofengand
[53] Replacement of odd bases in tRNA by fluorescent dyes Original Research Article
Pages 667-673
W. Wintermeyer, H.G. Zachau
[54] Photo-induced cross-linking of 4Srd and Cyd residues in Escherichia coli tRNA and its use as a conformational probe Original Research Article
Pages 673-684
J. Ofengand, P. Delaney, J. Bierbauivi
[55] The chemical preparation of acetylaminoacyl-tRNA Original Research Article
Pages 685-688
Sara Rappoport, Yehuda Lapidot
[56] The synthesis of oligopeptidyl-tRNA Original Research Article
Pages 688-695
Yehuda Lapidot, Sara Rappoport
[57] Peptidyl-tRNA analogs as affinity labeling agents Original Research Article
Pages 695-706
Daniel V. Santi, Stephen O. Cunnion
[58] Rabbit liver tRNA nucleotidyltransferase Original Research Article
Pages 706-716
Murray P. Deutscher
[59] tRNA methyltransferases Original Research Article
Pages 716-726
Sylvia J. Kerr
[60] Assay and purification of met-tRNA hydrolase from rabbit reticulocytes Original Research Article
Pages 726-739
Herry Morrisey, Boyd Hardesty
Nucleotide sequences of transfer ribonucleic acids
Pages 741-756
Mary Ann Sodd, B.P. Doctor
Author index
Pages 757-774
Subject index
Pages 775-804