EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany: Volume 3: Life Science

Front Matter....Pages I-XXII
Cryoelectron microscopy: from molecules to systems....Pages 1-2
Progress in High-resolution Scanning Probe Microscopy....Pages 3-4
Modular organization of RNase P....Pages 5-6
Molecular anatomy of the human pathogen Leptospira interrogans ....Pages 7-8
Unveiling conformational changes of biological molecules using multiscale modeling and multiresolution experiments....Pages 9-10
Nicolas Boisset: In memoriam....Pages 11-12
Cryo TEM-based 3D reconstruction of the recombinant expressed human zinc peptidase Meprin β....Pages 13-14
Origin melting by SV40 Large T antigen-new insights from 3D-EM-MLF3D classification method....Pages 15-16
Sequence analysis and modelling of the two large subunits of Phosphorylase Kinase....Pages 17-18
Cellular Uptake of Polymer Nanoparticles Imaged by Electron Microscopy....Pages 19-20
9 Å cryo-EM structure and molecular model of a gastropod hemocyanin didecamer (KLH1) reveals the architecture of the asymmetric collar....Pages 21-22
Microstructure of model systems for sauces based in polysacharides observed by Cryo-SEM....Pages 23-24
Three-dimensional architecture of outer- and inner-dynein arms in flagella revealed by cryo-electron tomography and single particle analysis....Pages 25-26
Structural and functional studies of rabbit skeletal muscle Phosphorylase Kinase....Pages 27-28
Single-particle analysis of the Cdc6/Orc1, archaeal DNA replication initiator protein....Pages 29-30
A high-throughput acquisition toolbox in Matlab for cryo-electron microscopy studies....Pages 31-32
Architecture of bacterial glutamate synthase complexe using hybrid approaches....Pages 33-34
3D Structure of DNA Repair Macromolecular Complexes Participating in Non-Homologous End-Joining (NHEJ)....Pages 35-36
Quaternary structure of recombinant human meprin β, a zinc peptidase of the astacin family....Pages 37-38
Allosterism of Nautilus pompilius hemocyanin as deduced from 8 Å cryo-EM structures obtained under oxy and deoxy conditions....Pages 39-40
High resolution structure of a 6 MDa protease by xray-crystallography and cryo-EM....Pages 41-42
Cryo-TEM of liquid crystalline particles: Application of materials science techniques to study liquid crystalline particle structures....Pages 43-44
Structural basis for the concerted integration of HIV-1 DNA in the human genome, role of the human cofactor LEDGF/p75....Pages 45-46
Titan Krios: Automated 3D imaging at ambient and cryogenic conditions....Pages 47-48
Self assembly and phase behaviour of new sugar based gemini amphiphiles....Pages 49-50
Peptide-nanotube formation by lysine based lipoamino acids....Pages 51-52
Advances in biological microanalysis using event streamed spectrum imaging and programmed beam acquisition....Pages 53-54
3D-correlation-averaging for membrane-protein-crystals....Pages 55-56
8 Å cryo-EM structure of the giant hemoglobin from the planorbid snail Biomphalaria glabrata ....Pages 57-58
Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha . Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport....Pages 59-60
Structure of OprM-MexA interacting complex revealed by cryo electron tomography....Pages 61-62
Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses....Pages 63-64
Combined structural and chemical analysis of unique anammox bacteria that contain a prokaryotic organelle....Pages 65-66
Structural and functional considerations on the 3-D organization of the fenestrated cytoplasm of hepatic endothelial cells....Pages 67-68
Molecular architecture of the presynaptic compartment studied by cryo-electron tomography....Pages 69-70
Synapses in high pressure frozen Caenorhabditis elegans ....Pages 71-72
High resolution EM-tomography of a whole vitrified cell....Pages 73-74
Evaluation of beam damage in catalase crystals observed in vitrified sections....Pages 75-76
Urothelial fusiform vesicles are formed in post-Golgi compartment....Pages 77-78
Localisation of GFP and GFP tagged PKD2 in cultivated pancreatic cancer cells using high-pressure freezing....Pages 79-80
Cytomegalovirus membrane envelopment revisited — a STEM-tomography study based on high-pressure freezing and freeze substitution....Pages 81-82
Electron tomography & template matching of biological membranes....Pages 83-84
Correlated Microscopy: From Dynamics to Structure....Pages 85-86
3DEM-analyses of Golgi apparatus subunits during physiologic and pathologic reorganizations....Pages 87-88
Visualization of the 80s ribosome in situ using cryo electron tomography of vitreous sections....Pages 89-90
Three-dimensional analysis of the intermediate filament network using SEM-tomography....Pages 91-92
The apical vesicles of the tuft cells in the main excretory duct of the rat submandibular gland by EFTEM-TEM tomography....Pages 93-94
(S)TEM Dual Axis Tomography....Pages 95-95
STEM tomography of high-pressure frozen cell monolayers....Pages 97-98
Multimodality of pericellular proteolysis in cancer cell invasion....Pages 99-100
Photonic crystal type nanoarchitectures in butterfly wing scales....Pages 101-102
Iridovirus-like viruses of Lacerta monticola from Serra da Estrela, Portugal....Pages 103-104
Structural and ultrastructural investigations on the in vitro effects of plant extracts in chicken and rabbit whole blood cultures....Pages 105-106
Fine structural characterization of cloned human endogenous retrovirus HERV-K113....Pages 107-108
The procaine hydrochlorate effect onto the corpuscular anthocyans from the vacuolar sap of different plant cells....Pages 109-110
Comparative anatomical and ultrastructural investigations on normal and vitroplantlets leaves from Pistia stratiotes L.....Pages 111-112
Autophagy of mitochondria in brown adipocytes of chemically thyroidectomised rats....Pages 113-114
Ultrastructural studies of immune organs in antigen primed chickens treated with vegetal extractions....Pages 115-116
Structural features of HIV-1 immunologic activation....Pages 117-118
Functions of actin-binding proteins in the cell nucleus....Pages 119-120
Ultrastructural mechanisms of entamoebae movement system....Pages 121-122
White adipocytes transdifferentiation into brown adipocytes induced by triiodothyronine....Pages 123-124
Ultrastructural studies of the tegument of cestodes (Platyhelminthes): phylogenetic implications....Pages 125-126
Crystallization stages of the CaCO 3 deposits in the earthworm’s calciferous gland....Pages 127-128
Scanning electron microscopic analysis of heavy metal resistant microorganisms....Pages 129-130
Characterization of mouse embryoid bodies by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM)....Pages 131-132
Stage-dependent localization of mitochondrial DNA during the cell cycle of Euglena gracilis Z by immunogold electron microscopy....Pages 133-134
The virus-inducted structures in RNA virus infected macrophages....Pages 135-136
Surface layers of ore-leaching Bacteria and Archaea....Pages 137-138
Structure of Bacillus anthracis spores analyzed by CEMOVIS....Pages 139-140
Insights into the role of pUL71 during HCMV morphogenesis....Pages 141-142
Fine structure of the genital chamber of female Chrysomya megacephala (F.) (Diptera: Calliphoridae)....Pages 143-144
Ultrastructure of the testes and spermatozoa of the blow fly Chrysomya megacephala (F.) (Diptera:Calliphoridae)....Pages 145-146
Biosynthesis of cellulose in red algae....Pages 147-148
Light and electron microscopy aspects of the glandular sessile hairs from the vitroplantlet leave of Drosera rotundifolia ....Pages 149-150
Ultrastructure of selected Euglena species in relation to their taxonomy....Pages 151-152
Confocal Microscopy Reveals Molecular Interactions Between DNA-binding Drugs and Chromatin in Live Cells....Pages 153-154
Fluorescence microscopy methods for measuring the mobility and stability of molecules in 3-D samples....Pages 155-156
Phasor-FLIM analysis of FRET for homotypic and heterotypic non-covalent interactions: stimulated membrane receptors in live cells....Pages 157-158
 In vitro immunofluorescence and ultrastructural analysis of the expression of noncollagenous matrix proteins in human pulpal cells induced by mineralizing factors....Pages 159-160
Differentiation-dependent Golgi fragmentation in the bladder urothelial cells in vitro....Pages 161-162
Monitoring Mitochordria Dynamics in Living Cell System by Confocal Microscopy....Pages 163-164
The freeze-fracture replica immunolabeling technique for the study of lipid droplet biogenesis and function....Pages 165-166
Dynamics of epigenome duplication, accessibility and translation....Pages 167-168
Controlled light exposure microscopy reveals telomeric microterritories throughout the cell cycle....Pages 169-170
Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic....Pages 171-172
Spectral methods for cells and particles identifications....Pages 173-174
3D realistic visualization of supramolecular assemblies....Pages 175-176
Heterosis in Arabidopsis thaliana: Structural aspects in mature and germinating seeds from hybrid and parental lines....Pages 177-178
Colour visualization of red blood cells in native smears by the new method reflected light microscopy....Pages 179-180
 In vitro culture of Trigonella foenum-graecum plantules and their anatomic characterization....Pages 181-182
Live cell imaging by SEM-hosted X-ray microscope in “water window” energy range....Pages 183-184
Microscopic anatomy and mineral composition of cuticle in amphibious isopods Ligia italica and Titanethes albus (Crustacea:Isopoda)....Pages 185-186
In vivo imaging and quantification of the continuous keratin filament network turnover....Pages 187-188
Necrotic cell death, a controlled way of cellular explosion....Pages 189-190
Dissecting mitochondrial protein distributions using sub-diffraction resolution fluorescence microscopy....Pages 191-192
High Thoughput, High Content Tissue Cyotometry....Pages 193-194
Focus on the vascular wall: Imaging of large arteries using two-photon microscopy....Pages 195-196
High resolution confocal Ca 2+ imaging of the pulmonary neuroepithelial body microenvironment in lung slices....Pages 197-198
Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering....Pages 199-200
Testing calibration standards for confocal and two-photon microscopy....Pages 201-202
Distribution of the voltage-gated delayed-rectifier K + subunits, K v 1.1 and K v 1.2, in the adult murine enteric nervous system....Pages 203-204
Observation of Ventricular Myocyte Morphology in Long Term Culture using High Resolution Confocal Imaging....Pages 205-206
A magnetic field enhances the inmunofluorescence signal in a lymphocytic cell....Pages 207-208
Novel 515 nm DPSS-laser brings excitement in life cell imaging....Pages 209-210
Confocal laser scanning microscopy study on human fissure caries stained with alizarin red....Pages 211-212
Non-invasive skin tissue characterization using non-linear spectral imaging microscopy....Pages 213-214
Force Spectroscopy reveals mechanical structure of capsids....Pages 215-216
Manipulating and imaging molecular motors with optical traps, single-molecule fluorescence and atomic force microscopy....Pages 217-218
Mechanical stability of ethanol-induced A-DNA....Pages 219-220
Manipulation of the mechanical properties of a virus by protein engineering....Pages 221-222
A single-molecule mechanical assay to study DNA replication coupled to DNA unwinding....Pages 223-224
Generation of multiple DNA lesions at subnuclear resolution by multi-photon irradiation....Pages 225-226
Light and electron microscopy of phagosomes....Pages 227-227
The Use of Electron Microscopy in the Diagnosis of Human Microsporidial Infection — the Manchester, UK, Experience....Pages 229-230
Overview of the impact of biological microanalysis in health and disease....Pages 231-232
Immunolocalization of FOXP3 in HCV-infected liver biopsies. Preliminary observations.....Pages 233-234
Ultrastructural and Intracellular Elemental Composition Analysis of Human Hematopoietic Cells During Cold Storage in Preservation Solutions....Pages 235-236
Reconstitution and morphological characterisation of an original Human Endogenous Retrovirus....Pages 237-238
Cell death in human articular chondrocyte: an ultrastructural study in micromass model.....Pages 239-240
Pathomorphological diagnostic of paraffin embedded versus epon embedded cardiac tissues with transmission electron microscope analysis....Pages 241-242
Interaction of carbon nanotubes with macrophages: STEM and EELS approach....Pages 243-244
The ultastructural effect of nitrogen mustard gas on rat brain and therapeutic value of proanthocyanidin....Pages 245-246
Assembly and maturation of pestiviruses....Pages 247-248
Analysis of radiation-induced bystander effects using high content screening....Pages 249-250
Electron microscopic investigations on normal and dexamethasone applied rat placentas....Pages 251-252
Changes in dental enamel birefringence after CO 2 laser irradiation through fluoride gel -a pilot study....Pages 253-254
SEM and light microscopic examination and morphometric analysis of temporal changes in intimal and medial connective tissue component of human carotid arteries....Pages 255-256
Ultrastructure of human amniotic membrane covering villous chorion and smooth chorion in preeclampsia....Pages 257-258
Changes in Intracellular Sodium, Chlorine, and Potassium Content in Hematopoietic Cells after Hypotermic Storage....Pages 259-260
Requirements for a bright future of electron microscopy in the rapid laboratory diagnosis of infectious diseases....Pages 261-262
Changed thickness of zona pellucida as a result of stimulated ovulation....Pages 263-264
Mozaic microscopy of pancreatic islets....Pages 265-266
Microscope history Database....Pages 267-268
Interaction of H-Ras Transformed Cell Line with Folic Acid Modified Magnetic Nanoparticles and Detection by Transmission Electron Microscope....Pages 269-269
Sedimentation of suspensions for diagnostic thin section electron microscopy....Pages 271-272
Evaluation of rofecoxib administration influence on ultrastructural image of the kidney....Pages 273-274
Microstructural changes produced by Pulsed Electric Fields in liquid whole egg studied by Transmission Electron Microscopy (TEM)....Pages 275-276
Microstructural changes in dough treated by Glucose oxidase (GOX) and Transglutaminase (TG), studied by Scanning Electron Microscopy (SEM)....Pages 277-278
Cytoplasmic K/Na balance in the cardiomyocyte of young and old rats....Pages 279-280
Hyperspectral imaging — a novel concept for marker free chromosome characterization....Pages 281-282
Poxviruses: Morphogenesis of an Orthopoxvirus (CPXV) and an Avipoxvirus (FPXV) in host tissue....Pages 283-284
Ultrastructural alterations of methotrexate in mouse kidney....Pages 285-286
Ultrastructural gadolinium evidence in skin of a patient with Nephrogenic Fibrosing Dermopathy....Pages 287-288
Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study....Pages 289-290
Tissue distribution of peroxisomes in zebrafish....Pages 291-292
An analysis of the ultrastructure of matured HIV....Pages 293-294
 Trichinella spiralis and Trichinella britovi cuticle and hypodermal glands Ultrastructure....Pages 295-296
Ultrastructural analysis of lysosomal storage diseases: effects of therapy....Pages 297-298
Immunolabeling for Electronmicroscopy....Pages 299-300
Molecular Organisation of Cadherins in Intercellular Adhesion Junctions by Cryo-Electron Tomography of Vitreous Sections....Pages 301-302
Ultrastructural observations of indium in the lactating mammary gland cells....Pages 303-304
An active mechanism flanks and modulates the export of the small ribosomal subunits....Pages 305-306
New methods for micro-domain detection in bacterial sacculi....Pages 307-308
Subcellular localization of Myogenic Regulatory Factors along skeletal muscle development....Pages 309-310
Immunocytochemical Strategies: LR Resins or Lowicryl, Gold or Peroxidase — Which is Better?....Pages 311-312
Elemental Analysis in Electron Microscopy for Medical Diagnostics....Pages 313-314
Synaptic localization of KCa1.1 potassium channels in central neurons revealed by postembedding immunogold and SDS-digested freeze-fracture replica labelling....Pages 315-316
Ultrastructural studies of rod photoreceptor nuclei from SCA7 mouse....Pages 317-318
Application of electron energy loss spectroscopy for heavy metal localization in unicellular algae....Pages 319-320
Localization of “flagella” proteins in the Archaeon Ignicoccus hospitalis ....Pages 321-322
Detection of gold nanoparticles by Autrata YAG detector in FE SEM working in cryo mode....Pages 323-324
Correlative cryo-fluorescence and electron microscopy....Pages 325-326
The effect of hypometabolizing molecules on transcription as shown by a new two-step pulse-chase method....Pages 327-328
Cryofixation and Freeze-Substitution Combined with Tokuyasu Cryo-section Labelling....Pages 329-330
The Drosophila active zone architecture: combining confocal, STED and transmission electron microscopy....Pages 331-332
Distribution and function of amorphous CaCO 3 and calcite within the tergite cuticle of terrestrial isopods (Crustacea)....Pages 333-334
A symbiosis: tracking cell signaling with expression probes, quantum dots and a programmable array microscope (PAM)....Pages 335-336
Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy....Pages 337-338
Understanding the 3D architecture of organelle bound protein complexes using cryo-electron tomography of frozen hydrated sections and immunogold; our next great challenge....Pages 339-340
Cryo-electron microscopy of vitreous sections....Pages 341-341
The visualization of viruses in the low-voltage electron microscope....Pages 343-344
Exploring HPF/FS methods for sensitive immunogold labeling on resin sections....Pages 345-346
Low contrast of the ER-membranes in the high pressure frozen, freeze substituted specimens....Pages 347-348
HPF of cultured cell monolayers: towards a standard method for high pressure freezing, freeze substitution and electron tomography acquisition....Pages 349-350
Life-like physical fixation of large samples for correlative microscopy....Pages 351-352
Integrating automated single, double and triple in situ molecular detection with imaging for the analysis of inter- and intracellular events in the seminiferous tubule....Pages 353-354
Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation....Pages 355-356
The Shape of Caveolae is Omega-like after Chemical Fixation and Cup-like after Cryofixation....Pages 357-358
Integrated laser and transmission electron microscopy....Pages 359-360
Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus....Pages 361-362
Strategies for the morphological analysis of hydrated and life-like preserved biomedical material in the SEM....Pages 363-364
Denaturation of metaphase chromatin plates observed by transmission electron microscopy and atomic force microscopy in aqueous solution....Pages 365-366
Discovery of a Nuclear Structure in UVC-induced Apoptotic Cells by Integrated Laser Electron Microscopy....Pages 367-368
Analysis of biomineral formation in three-dimensional micro-mass stem cell cultures....Pages 369-370
Comparative structural studies of highly-filled polymethacrylates with light microscopy, SEM, TEM and AFM....Pages 371-372
Cell surface characteristics as reporters for cellular energy state....Pages 373-374
Analysis of multimodal 3D microscopy measurements....Pages 375-376
Permanent plastid — nuclear complexes (PNCs) in plant cells....Pages 377-378
Cell imaging by dynamic secondary ion mass spectrometry (SIMS): basic principles and biological applications....Pages 379-380
Water imaging in cells cryosections by electron energy loss spectroscopy....Pages 381-382
Study of the cellular uptake of Pt nanoparticles in human colon carcinoma cells....Pages 383-384
Ion spectrometry and electron microscopy correlative imaging for accurate comprehensive trace element localization at subcellular level, in normal and pathological keratinocytes....Pages 385-386
Calcium Spark Detection and Analysis in Time Series of Two-Dimensional Confocal Images....Pages 387-388
Visualisation of the attachment, possible uptake and distribution of technical nanoparticles in cells with electron microscopy methods....Pages 389-390
Back Matter....Pages 391-407