Electroporation and Sonoporation in Developmental Biology

Front Matter....Pages i-xxix
Short History of Electroporation for the Study of Developmental Biology....Pages 3-7
In Ovo Electroporation as a Useful Tool to Pursue Molecular Mechanisms of Neural Development in Chick Embryos....Pages 9-16
Applications of Tol2 Transposon-Mediated Gene Transfer for Stable Integration and Conditional Expression of Electroporated Genes in Chicken Embryos....Pages 17-24
Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systems in Vertebrate Embryos....Pages 25-36
In Ovo Electroporation for Targeting the Somitic Mesoderm....Pages 37-42
Method of Electroporation for the Early Chick Embryo....Pages 43-53
Enhancer Analysis....Pages 55-71
Electroporation of Nucleic Acids into Chick Endoderm Both In Vitro and In Ovo....Pages 73-83
Electroporation into the Limb: Beyond Misexpression....Pages 85-96
Retinal Fiber Tracing by In Ovo Electroporation....Pages 97-104
Retroviral Vector-Mediated Gene Transfer into the Chick Optic Vesicle by In Ovo Electroporation....Pages 105-116
Clonal and Widespread Gene Transfer by Proviral Electroporation for Analysis of Brain Laminar Formation....Pages 117-127
Electroporation into Cultured Mammalian Embryos....Pages 129-141
In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neurons....Pages 143-152
Practical Application of Microelectroporation into Developing Mouse Brain....Pages 153-167
Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures....Pages 169-177
Temporal and Tissue-Specific Control of Gene Expression in the Peri-Implantation Mouse Embryo Through Electroporation of dsRNA....Pages 179-200
Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderm Determination Towards a Haematopoietic Fate....Pages 201-217
Electroporation of Embryonic Kidney Explants....Pages 219-227
Transfer of Foreign DNA into Aquatic Animals by Electroporation....Pages 229-237
Electroporation in the Regenerating Tail of the Xenopus Tadpole....Pages 239-247
Electrotransfer of Plasmid Vector DNA into Muscle....Pages 249-262
Bone Formation by BMP Gene Transfection....Pages 263-270
Electroporation of the Testis....Pages 271-283
Direct Gene Transfer into Plant Mature Seeds via Electroporation After Vacuum Treatment....Pages 285-293
RNA Interference in Chicken Embryos....Pages 295-314
Sonoporation in Developmental Biology....Pages 317-326
Back Matter....Pages 327-331