Drug Metabolite Isolation and Determination

Front Matter....Pages i-xii
Front Matter....Pages 1-1
Strategies Centered on HPLC....Pages 3-11
Preparative Liquid-Chromatographic Methods in Drug Metabolism Studies....Pages 13-32
Problems Arising from Assaying Low Concentrations by HPLC....Pages 33-40
Extraction of Drugs and Metabolites from Plasma and Urine....Pages 41-46
The Use of Long Capillary GC Columns for Urinary Phenolic Acids from Catecholamines and Analogues....Pages 47-54
ADDENDUM: Mean excretions of phenolic acids in man....Pages 54-54
Quantitative HPTLC in Assaying Body Fluids for Drugs and Metabolites....Pages 55-62
Front Matter....Pages 63-63
 A Note on TLC Densitometry for Drug and Metabolite Determination....Pages 65-67
 A Note on Taking Aliquots from Small Volumes of Upper Phases....Pages 69-69
 A Note on Rapid Removal of Fine Particles from Alumina for Column Chromatography....Pages 71-73
 A Note on Assessment of Radioactivity Measurements on HPLC Effluents....Pages 75-80
 A Note on Quantitation of 14 C Radioactivity in HPLC Eluates....Pages 81-88
Front Matter....Pages 95-95
Examples of Approaches to Isolating and Identifying Phase I Metabolites....Pages 97-102
The Identification of Amitriptyline and Benzodiazepine Metabolites....Pages 103-110
A Novel Use of RIA and HPLC in the Identification of Plasma Metabolites of HR 158....Pages 111-118
Investigation of Pesticide Metabolites in the Human....Pages 119-133
Front Matter....Pages 135-135
 A Note on Metabolic Fate of the Immunoadjuvant Peptidoglycan Monomer....Pages 137-139
 A note on HPLC as a Means of Following an in vitro Synthesis of 7-Hydroxymethotrexate....Pages 141-142
Front Matter....Pages 147-147
Isolation of Conjugates and Identification of the Attached Groups....Pages 149-160
Isolation and Characterization of Amino Acid and Sugar Conjugates of Xenobiotic Carboxylic Acids....Pages 161-179
Front Matter....Pages 147-147
Chromatography, Hydrolysis and Rearrangement of the Glucuronide Conjugate of Isoxepac, as Affecting its Assay in Urine....Pages 181-187
Front Matter....Pages 189-189
 A Note on Isolation and Identification of a Drug Conjugate....Pages 191-196
Front Matter....Pages 199-199
HPLC Analysis of the Major Urinary Metabolite of Flurazepam....Pages 201-206
Quantification of Metabolites of Aminopyrine and Antipyrine in Plasma and Urine....Pages 207-214
Immunoassay of Bromocriptine and Specificity of Antibody: Criteria for Choice of Antiserum and Marker Compound....Pages 215-223
Determination of 3-Phenoxy- N -Methylmorphinan and Metabolites in Plasma....Pages 225-230
Assays where High Sensitivity was Achieved, Especially by HPTLC with Automatic Spotting and Colour Reactions....Pages 231-238
Front Matter....Pages 239-239
 A Note on Selective Column-Extraction of Psycho-Active Drugs and Metabolites....Pages 241-242
 A Note on Determination of Atracurium and Its Metabolites....Pages 243-246
 A Note on Discrimination Between Carbon Monoxide as a Metabolite of Carbon Tetrachloride and as a Degradation Product of the Biological Material....Pages 247-248
 A Note on HPLC Determination of Cefotaxime and Its Metabolites....Pages 249-249
Metabolite Methodology: The State-of-Play....Pages 255-270
Corrections to Vol. 10, Trace-Organic Sample Handling ....Pages 289-289
Corrections to Vol. 11, Cancer-Cell Organelles ....Pages 289-289
Back Matter....Pages 271-289