Doubled Haploid Production in Crop Plants: A Manual

Front Matter....Pages N1-xlvi
Production of doubled haploids in crop plants. An introduction....Pages 1-4
Laboratory protocol for anther culture technique in rice....Pages 109-116
Triticale anther culture....Pages 117-121
Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm.)....Pages 123-128
Protocol of triticale (x Triticosecale Wittmack) microspore culture....Pages 129-134
Doubled haploid production in barley using the Hordeum bulbosum (L.) technique....Pages 5-14
The Hordeum bulbosum (L.) method....Pages 15-19
Anther culture in barley....Pages 21-27
Barley anther culture....Pages 29-34
Anther culture for doubled haploid production in barley ( Hordeum vulgare L.)....Pages 35-42
Barley isolated microspore culture protocol....Pages 43-47
Barley isolated microspore culture (IMC) method....Pages 49-52
Doubled haploid production in wheat through wide hybridization....Pages 53-58
Protocol for wheat ( Triticum aestivum L.) anther culture....Pages 59-64
Protocol for producing doubled haploid plants from anther culture of wheat ( Triticum aestivum L. ) ....Pages 65-70
Wheat anther culture....Pages 71-76
Haploid wheat isolated microspore culture protocol....Pages 77-81
Production of doubled haploids in wheat ( Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals....Pages 83-94
Isolated microspore culture in maize ( Zea mays L.), production of doubled-haploids via induced androgenesis....Pages 95-102
Anther culture of maize ( Zea mays L.)....Pages 103-108
Protocol for broccoli microspore culture....Pages 195-204
Haploid and doubled haploid production in durum wheat by wide hybridization....Pages 161-166
Haploid and doubled haploid production in durum wheat by anther culture....Pages 167-172
 In vitro gynogenesis induction and doubled haploid production in onion ( Allium cepa L.)....Pages 275-279
Haploid production of potatoes by anther culture....Pages 229-234
Anther culture through direct embryogenesis in a genetically diverse range of potato ( Solanum ) species and their interspecific and intergeneric hybrids....Pages 235-240
Potato haploid technologies....Pages 241-247
Microspore culture in rapeseed ( Brassica napus L.)....Pages 185-193
Anther culture and isolated microspore culture in timothy....Pages 173-177
Microspore culture of Brassica species....Pages 205-215
Protocol for microspore culture in Brassica....Pages 217-222
Anther culture of linseed ( Linum usitatissimum L.)....Pages 249-254
Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize....Pages 135-140
Doubled haploid induction in ryegrass and other grasses....Pages 179-183
Protocol for rye anther culture....Pages 141-149
Microspore culture of rye....Pages 151-154
Doubled haploid production of sugar beet ( Beta vulgaris L.)....Pages 255-263
Anther and microspore culture in tobacco....Pages 223-228
Asparagus microspore and anther culture....Pages 265-273
Oat haploids from wide hybridization....Pages 155-159
Haploid induction in onion via gynogenesis....Pages 281-285
Haploids and doubled haploids in Citrus ssp.....Pages 303-307
Oak anther culture....Pages 297-301
Doubled haploid production in poplar....Pages 293-296
 In vitro androgenesis in apple....Pages 287-292
Published doubled haploid protocols in plant species....Pages 309-335
Doubled haploids in breeding....Pages 337-349
Doubled haploid mutant production....Pages 351-361
Barley microspore transformation protocol by biolistic gun....Pages 363-366
Doubled haploids in genetic mapping and genomics....Pages 367-390
Cytogenetic tests for ploidy level analyses — chromosome counting....Pages 391-395
Ploidy determination using flow cytometry....Pages 397-403
Major media composition....Pages 405-413
Basic equipment for maize microspore culture laboratory....Pages 415-416
Back Matter....Pages 417-428