Diagnosis and Identification of Plant Pathogens: Proceedings of the 4th International Symposium of the European Foundation for Plant Pathology, September 9–12, 1996, Bonn, Germany

Front Matter....Pages i-xx
Front Matter....Pages 1-1
The importance of diagnosis and prognosis in integrated plant protection....Pages 3-8
Phytosanitary Implications of Diagnostic Activities and Vice Versa....Pages 9-13
General Aspects on diagnostic Methods: Viruses Microorganisms....Pages 15-16
Fungal Taxonomy in Crisis....Pages 17-21
PCR-Based Diagnostics of Agricultural Pests and Diseases....Pages 23-28
Front Matter....Pages 29-29
Electron microscopic methods currently used for diagnosis and characterization of plant viruses....Pages 31-34
Experiences with the Isolation of Plant Pathogenic Fungi....Pages 35-36
Detection of Different Bacterial and Fungal Pathogens in Apparently Healthy Strawberry Plants....Pages 37-41
The Use of Nested PCR for the Detection of Phytophthora Fragariae in Strawberry....Pages 43-45
rDNA Sequencing: An Essential Guide to the Taxonomy and Diagnosis of Colletotrichum....Pages 47-51
Anastomosis Grouping and Molecular Genetic Typing of Rhizoctonia Solani Causing Crater Disease of Wheat in South Africa....Pages 53-57
Identification of Physiologic Races and Formae Speciales of Fusarium Oxysporum ....Pages 59-62
Fatty Acid Analysis in the Identification, Taxonomy and Ecology of (Plant Pathogenic) Bacteria....Pages 63-70
Assessment of Microbial Communities by Fatty Acid Analysis....Pages 71-74
Testing Viability and Germination of Plasmodiophora Brassicae (Wor.) Resting Spores Using the Fluorescence Microscope Technique....Pages 75-78
Histological Study of a Susceptible Potato Cultivar ( Solanum tuberosum L. cv. Igor) Infected with Potato Virus Y NTN ....Pages 79-84
Assessment of Characteristics for Determining Pathogen Populations of Rhizoctonia Solani in Rice-Based Cropping Systems....Pages 85-88
Front Matter....Pages 89-89
Monoclonal Antibody-Based Assays for Detection and Quantification of Botrytis Cinerea ....Pages 91-94
Sensitive Detection of Plant Pathogens by Using Immobilized Targets in Tissue Imprinted Membranes....Pages 95-98
Development of a Dot-Immunobinding Assay for the Detection of Phytophthora spp. in Roots....Pages 99-100
Front Matter....Pages 89-89
Detection of Resting Spores of Spongospora Subterranea F.SP. Subterranea Using Serological Techniques....Pages 101-104
Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Verticillium Dahliae Detection in Soil....Pages 105-108
Immunological Methods for the Detection of Verticillium Dahliae in Oilseed Rape....Pages 109-112
Development of a Rapid Method for The Detection and Identification of Colletotrichum Acutatum ....Pages 113-116
Selective Enrichment Improves Isolation, Serological and Molecular Detection of Plant Pathogenic Bacteria....Pages 117-121
Application of Different Techniques to Detect Phytophthora spp. in Roots of Nursery Plants....Pages 123-124
Serological Detection of Spongospora Subterranea F.SP. Subterranea in Soil....Pages 125-128
Prospects of Using PCR for the Detection of Bacterial Pathogens in Potato....Pages 129-135
Development of a Semi-Selective Medium and Use of Serology for Fast and Sensitive Detection of Xanthomonas Campestris PV. Manihotis , Incitant of Bacterial Blight of Cassava....Pages 137-140
Characteristics of Large-Scale Production of Phyto-Sanitary Improved In-Vitro Fruit Tree Cultivars Using In-Vitro Elimination Treatments and Serodiagnostics....Pages 141-147
Production of antibodies against a synthetic peptide based on a sequence common to all tospovirus non-structural Ns S protein....Pages 149-154
Front Matter....Pages 155-155
The use of the polymerase chain reaction for the characterisation and diagnosis of yam potyviruses....Pages 157-160
Novel Methods Based on Genomic Amplification for Routine Detection of Potato Leafroll Virus in Potato Tubers....Pages 161-167
Routine Detection of Chrysanthemum Stunt Viroid in Chrysanthemum Plants by Reverse Transcription - Polymerase Chain Reaction....Pages 169-172
Identification of a Tobamovirus Infecting New Guinea Impatiens ....Pages 173-173
IC-RT-PCR Detection of Blackcurrant Reversion Associated Nepovirus, and Perspectives of Its Use for Diagnosis of the Reversion Disease....Pages 175-178
Detection and Identification by PCR-Based Techniques of Diverse Phytoplasmas Infecting Grapevine....Pages 179-182
Diagnosis of Viroids in Fruit Trees: An Italian Experience....Pages 183-187
Detection of Plant Pathogenic Bacteria in Ornamentals by the PCR Technique....Pages 189-192
Differentiation and Detection of the Subspecies of Clavibacter Michiganensis by PCR (Polymerase Chain Reaction) - Techniques....Pages 193-193
Front Matter....Pages 155-155
Molecular diagnosis of rape seed pathogens....Pages 195-198
Identification of Space-Specific Markers of Phytophthora Fragariae with AFLP....Pages 199-201
Physiological Race Determination in Fusarium Oxysporum F.SP. Dianthi by RAPD-PCR....Pages 203-206
Quantitative Estimation of Fusarium Graminearum Using a Novel Solid Phase PCR-Assay....Pages 207-211
Molecular Diagnosis of Toxigenic Fusarium Species....Pages 213-217
Genetic Variability of Ascochyta Rabiei : Population Structure Revealed by RAPD Analysis and Classification into Pathotypes....Pages 219-222
Detection of Lophodermium Seditiosum from Pine Needles Using PCR Techniques....Pages 223-226
Front Matter....Pages 227-227
A Common Basis of Genetically Based and Induced Resistance in Cereals....Pages 229-235
Quantification of Fungal Biomass of a Pathogen and an Antagonist in Necrotic Leaf Tissue Using Fluorescence Microscopy and Image Analysis....Pages 237-238
A Novel Spore Trap for Monitoring Airborne Alternaria Brassicae Spores Using Monoclonal Antibodies....Pages 239-242
Fusarium Ear Blight on Wheat - Epidemiology and Control of Gibberella Zeae , The Teleomorph of Fusarium Graminearum , with Folicur®....Pages 243-246
Risk Assessment Through Quantitative Detection of Soil Suppressiveness Against Plant Diseases Caused by....Pages 247-251
Effect of Crop on the Diversity and the Potential of Fungal Egg Pathogens of Cyst Nematodes....Pages 253-255
Modified Spore Germination Inhibition Technique for Evaluation of Candidate Fungitoxicant ( Eucalyptus spp.)....Pages 257-263
Front Matter....Pages 265-265
Introducing Decision Support Systems Available in Germany....Pages 267-270
Estimation of Disease Severity Using Redefined Disease Incidence....Pages 271-274
A Decision Support System for Cercospora Leaf Spot on Sugarbeet....Pages 275-279
An Advisory System for the Control of Brown Rust on Winter Wheat in Northern Italy....Pages 281-284
Vegetative Compatibility Groups in Acremonium Cucurbitacearum ....Pages 287-289
Causal Agents of Hop-Dying in Spalt....Pages 291-293
Front Matter....Pages 265-265
 Fusarium Sections Elegans and Liseola : Taxonomy, rDNA Internal Transcribed Spacer Sequences and Diagnostics....Pages 295-297
Investigation of Claviceps Purpurea /FR./ Tul in the Poacea Family Plants in Lithuania....Pages 299-301
First Report on Anthracnose Diagnosis and Colletotrichum spp. Identification in White Lupin ( Lupinus Albus L.) Infection in Poland....Pages 303-306
Suitability of Electron Microscopy for Routine Diagnosis of Plant Virus Diseases in Extension Service....Pages 307-309
Use of the Scanning Electron Microscope in the Identification of Powdery Mildews....Pages 311-313
Analysis of Pyrenophora Teres (Died.) Drechs. Population from Slovakia....Pages 315-316
Detection of Plum Pox Virus by Different Methods....Pages 317-319
Image Analysis and Archiving as an Aid to Fungal Identification....Pages 321-323
Anastomosis Groups and Pathogenicity of Rhizoctonia Solani Isolates From Tobacco in Italy....Pages 325-327
Use of Fungicide-Resistance for the Characterisation and Differentiation of Venturia Inaequalis Strains - Suitability for Competitivity Studies....Pages 329-332
Isolation of Micromycetes from Pea Seeds....Pages 333-336
Isolation and Identification of Fusarium Spp. and Microdochium Nivale from Winter Wheat....Pages 337-340
Quantification of Phytophthora Infestans During Early Stages of Pathogenesis....Pages 341-343
Description of Microbial Communities of Rhizosphere Soil Based on the Phospholipid Fatty - Acid Spectrum Analysis of Microorganisms....Pages 345-348
Scanning Electron Microscopy of Verticillium Dahliae on the Cotton Root Surface....Pages 349-351
Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF)....Pages 353-356
Analysis of AMF- Glomus Caledonium J 55-Host Interaction — Activity, Isozyme Patterns and Plant Response....Pages 357-360
Antagonism Between Mycorrhizal Fungi and Heterobasidion Annosum (Fr.) Bref. - Scanning Electron Microscopical Studies....Pages 361-363
Pathogenicity of the Isolates of Rhizoctonia Cerealis and R. Solani from the Poznan Region Towards the Wheat Seedlings....Pages 365-367
The Presence of Spongospora Subterranea F.SP. Subterranea in the Northern Areas of Pakistan Confirmed by Microscopy, Serology and Bioassay....Pages 371-373
Front Matter....Pages 265-265
Differentiation and Immunochemical Characterisation of Various Potato Viruses and their Strains by Means of Monoclonal Antibodies....Pages 375-376
In sig ht ® - A New Diagnostic Tool for Mycosphaerella spp . in Banana Leaves....Pages 377-383
Feasibility of a Diagnostic Kit for Multiplex DIG-Chemiluminescent Detection of Tomato Viruses....Pages 385-389
Development of Monoclonal Antibodies for the Detection and Identification of Monilinia spp. Causing Brown Rot of Stone and Pome Fruit....Pages 391-393
Determination of Clavibacter Michiganensis subsp. Insidiosus Infection in Lucerne Seeds by Immunofluorescence Staining and Direct Isolation Using Selective Medium....Pages 395-397
Detection of Ryegrass Mosaic Virus by Monoclonal Antibodies and Immunocapture PCR....Pages 399-402
Detection of Poplar Mosaic Carlavirus (POPMV) by Immuno-Capture RT-PCR....Pages 403-405
Indirect Immunofluorescence - an Useful Method in Studies on Some Fungal Pathogens....Pages 407-409
The Use of Physiological and Biochemical Parameters of Plants for the Estimation of the Resistance to Stem Rust....Pages 411-413
Detection and differentiation of Three potyviruses infecting sweet potato by PCR....Pages 417-419
Differentiation of Fusarium Species by Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Ribosomal DNA....Pages 421-424
Diagnosis and Identification of Pathogens Causing Apple Storage Rots, by Using PCR-RFLP and Allele Specific Amplification....Pages 425-428
Development of DNA-assays for in Vitro differentiation and in Planta detection of Fusarium culmorum and Fusarium graminearum ....Pages 429-432
Isolation and Characterization of a Nepo-Type Virus from Reverted Blackcurrant ( Ribes nigrum L.)....Pages 433-435
Differential Detection of Erwinia Amylovora Isolates from Fruit Trees and Rubus spp. Based on Ribosomal DNA and PCR....Pages 437-439
Application of Polymerase Chain Reaction (PCR) for the Positive Identification of Two Phytoplasma Diseases in Poland....Pages 441-443
Silicacapture-Reverse Transcription-Polymerase Chain Reaction (SC-RT-PCR): Application for the Detection of Several Plant Viruses....Pages 445-448
Non-Radioactive Probe for the Detection of Strawberry Vein Banding Virus....Pages 449-451
Detection of Bacterial Diseases of Tomatoes by the Polymerase Chain Reaction (PCR) and Serology....Pages 453-455
Detection of Agrobacterium tumefaciens in Soil Using PCR....Pages 457-460
Front Matter....Pages 265-265
Sequence Analysis of the Ribosomal DNA Spacer Region of Pyrenophora spp.; Evaluation of its Potential for PCR Detection in a Seed Health Test....Pages 461-463
Identification of Potyviruses Infecting Alstroemeria ....Pages 465-467
Differentiation of Allium Potyviruses by Polymerase Chain Reaction....Pages 469-471
Detection and Differentiation of Sweet Potato Closteroviruses by RT-PCR and Single-Strand Conformation Polymorphism....Pages 473-475
The Use of Video Time-Lapse Recordings to Study the Effects of Antifungal Compounds on Phytopathogenic Fungi....Pages 479-481
Identification and Quantitative Determination of Phytophthora Species Infecting Ornamental Plants in Nurseries....Pages 483-485
Evaluation of Three Methods for the Detection of Septoria Spp. in Wheat Leaves....Pages 487-489
Variation for Resistance to Fusarium Head Blight ( Fusarium Culmorum ) in Somaclones of Winter Triticale....Pages 491-494
Quantification of Phytophthora Nicotianae on Tomatos Grown with The Nutrient Film Technique (NFT)....Pages 495-497
Comparison of Willow Leaf Rust Quantification Methods....Pages 499-501
Comparison Between a DNA-Based Assay and a Soil Bioassay in Quantifying the Amount of Gaeumannomyces Graminis Var. Tritici in Soil....Pages 503-505
Identification of Complex Pathotypes and Seasonal Changes of their Frequency in Subregional Populations of Barley Powdery Mildew ( Erysiphe Graminis F.SP. Hordei )....Pages 507-509
Characterization and Role in the Pathogenesis of Potatoes of a Novel Pectate Lyase from Eriwinia Chrysanthemi 3937....Pages 511-514
Fluorescent Parameters of Two Cucumber Cultivars Differing in Resistance to Mildew....Pages 515-517
The Rate Constants of Electron Transport in RC PS2 During Disease Development (Stem Rust, Powdery Mildew)....Pages 519-521
Rapid Assessment of Aggressiveness of Root Rot Agents of Alfalfa ( Medicago L.)....Pages 523-525
The Role of Prunus Spinosa L. in Epidemiology of Plum Pox Virus in the Czech Republic....Pages 527-530
Yield Reduction and Seed Transmission Rate of Lithuanian Isolate of Broad Bean True Mosaic Virus....Pages 531-533
Characterization of Fusarium Oxysporum Populations by Growth Parameter Evaluation in Microtiter Plates....Pages 535-538
Study on the Influence of Ascochyta Fabae and Botrytis Fabae on Two Growing types of Faba Bean....Pages 539-541
Back Matter....Pages 543-556