Diabetic Nephropathy: Methods and Protocols

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 Diabetic Nephropathy: An Overview  (Manpreet K. Sagoo, Luigi Gnudi)....Pages 3-7
Front Matter ....Pages 9-9
 Modeling Podocyte Biology Using Drosophila Nephrocytes  (Paul S. Hartley, Richard J. Coward)....Pages 11-24
 A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros  (Maria Kolatsi-Joannou, Daniel Osborn)....Pages 25-39
 Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model  (Carolynn Cairns, Bryan Conway)....Pages 41-52
 Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy  (Elisavet Vasilopoulou)....Pages 53-59
Front Matter ....Pages 61-61
 Histological Examination of the Diabetic Kidney  (Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti)....Pages 63-87
 Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy  (Kathryn E. White)....Pages 89-102
 Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy  (Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding)....Pages 103-126
Front Matter ....Pages 127-127
 Transcutaneous Measurement of Glomerular Filtration Rate in Rodents  (Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz)....Pages 129-137
 An In Vitro Method to Analyze Glucose Uptake in Podocytes  (Abigail C. Lay, Richard J. Coward)....Pages 139-143
 Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro  (Raina D. Ramnath, Simon C. Satchell)....Pages 145-151
 Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy  (Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann)....Pages 153-173
 Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease  (Karina Barreiro, Tobias B. Huber, Harry Holthofer)....Pages 175-188
 Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy  (Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires)....Pages 189-201
Front Matter ....Pages 203-203
 Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches  (Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, Amy Jayne McKnight)....Pages 205-240
 Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data—Association Studies  (Marisa Canadas-Garre, Laura J. Smyth, Kerry Anderson, Katie Kerr, Amy Jayne McKnight)....Pages 241-275
 Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients  (Lucy Jade Newbury, Alexa Wonnacott, Kate Simpson, Timothy Bowen, Donald Fraser)....Pages 277-285
 Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy  (Justyna Siwy, Linda Ahonen, Pedro Magalhães, Maria Frantzi, Peter Rossing)....Pages 287-306
Front Matter ....Pages 307-307
 Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice  (Ioannis Bantounas, Edina Silajdžić, Adrian S. Woolf, Susan J. Kimber)....Pages 309-322
 Generating Mutant Renal Cell Lines Using CRISPR Technologies  (Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David A. Long, Dagan Jenkins)....Pages 323-340
Back Matter ....Pages 341-342