Decellularized Scaffolds and Organogenesis: Methods and Protocols

Front Matter ....Pages i-xvi
 Decellularization Methods for Scaffold Fabrication  (Sweta K. Gupta, Narayan C. Mishra, Archna Dhasmana)....Pages 1-10
 Decellularized Liver Scaffold for Liver Regeneration  (Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, Lianhua Bai)....Pages 11-23
 Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications  (Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, Omar S. Aljitawi)....Pages 25-33
 3D Decellularized Native Extracellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells (hWJ MSCs)  (Balasubramanian Sundaram, Anne George Cherian, Sanjay Kumar)....Pages 35-53
 Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells  (Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, Gundula Schulze-Tanzil)....Pages 55-69
 Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells  (Takashi Hoshiba, Naoki Kawazoe, Guoping Chen)....Pages 71-86
 Mouse Skeletal Muscle Decellularization  (Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, Michela Pozzobon)....Pages 87-93
 Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering  (Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook)....Pages 95-102
 In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering  (Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Brambilla, Graziano Oldani, Francesco Calabrese et al.)....Pages 103-110
 Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds  (Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, Jin Mei)....Pages 111-119
 Preparation of Scaffolds from Decellularized Testicular Matrix  (Yoni Baert, Ellen Goossens)....Pages 121-127
 Decellularization of Bovine Small Intestinal Submucosa  (Mahmut Parmaksiz, Ayşe Eser Elçin, Yaşar Murat Elçin)....Pages 129-138
 Decellularization and Recellularization of Cartilage  (Catherine A. Bautista, Bahar Bilgen)....Pages 139-146
 A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration  (Jingting Li, Ming Pei)....Pages 147-154
 Tracheal Cartilage Isolation and Decellularization  (Zachary Galliger, Angela Panoskaltsis-Mortari)....Pages 155-160
 Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing  (Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, Mats Hellström)....Pages 161-175
 Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation  (Enes Taylan, Kutluk Oktay)....Pages 177-181
 Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component Within Hydrogels as In Vitro Models and Cell Delivery Systems  (Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, Lauren E. Flynn)....Pages 183-208
 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications  (Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson)....Pages 209-226
 Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze-Thaw Cycles and Detergent Treatment  (Susanne Pauline Roth, Ina Erbe, Janina Burk)....Pages 227-237
 Decellularized Bone Matrix Scaffold for Bone Regeneration  (Guobao Chen, Yonggang Lv)....Pages 239-254
 Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts  (Yibin Chen, Sharon Geerts, Maria Jaramillo, Basak E. Uygun)....Pages 255-270
 Decellularization of Liver and Organogenesis in Rats  (Nana Shirakigawa, Hiroyuki Ijima)....Pages 271-281
 Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold  (Matteo Ghiringhelli, Alessandro Zenobi, Stefano Brizzola, Fulvio Gandolfi, Valentino Bontempo, Sandro Rossi et al.)....Pages 283-292
 Liver Bioengineering Using Decellularized Whole-Liver Scaffolds  (Iris Pla-Palacín, Pilar Sainz-Arnal, Sara Morini, Manuel Almeida, Pedro M. Baptista)....Pages 293-305
 Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure  (Steven Skolasinski, Angela Panoskaltsis-Mortari)....Pages 307-315
 Alveolar Rhabdomyosarcoma Decellularization  (Michela Pozzobon, Mattia Saggioro, Stefania D’Agostino, Gianni Bisogno, Maurizio Muraca, Piergiorgio Gamba)....Pages 317-325
 Human-Scale Liver Harvest and Decellularization for Preclinical Research  (Kazuki Tajima, Hiroshi Yagi, Yuko Kitagawa)....Pages 327-335
 Erratum to: Decellularization Methods for Scaffold Fabrication  (Sweta K. Gupta, Narayan C. Mishra, Archna Dhasmana)....Pages 337-337
Back Matter ....Pages 339-347