Current Research Topics in Applied Microbiology and Microbial Biotechnology: Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (Biomicroworld2007)

Contents......Page 8
Introduction......Page 6
Agriculture, Soil, Forest Microbiology......Page 18
2. Materials and Methods......Page 20
3. Results......Page 21
4. Discussion......Page 23
References......Page 24
2. Materials and methods......Page 25
3. Results......Page 26
References......Page 28
2.2 Microbiological analysis......Page 29
3.2 Yeast populations in soils......Page 30
3.3.2 Fermentation of sterile must inoculated with soil samples......Page 31
References......Page 32
1. Introduction......Page 33
2.4. Analysis for Olpidium bornovanus......Page 34
3.2. Isolation using the “soil phytopathometry”......Page 35
4. Discussion......Page 37
Literature cited......Page 39
2. Materials and methods......Page 41
3.2 Effect of brown powder to the various plants during long term vegetation experiment......Page 42
3.3 Changes of microbial count in soil samples after vegetation experiment with brown powder treatment......Page 43
References......Page 44
2.1 Study area and soils......Page 46
2.3 Determination of kinetic parameters of denitrification enzymes......Page 47
3. Results and discussion......Page 48
References......Page 49
Material and methods......Page 50
Results and discussion......Page 51
References......Page 53
2. Materials and methods......Page 55
3.2 Effect of nutrient limitation on survival of R. solanacearum bv 2 in natural river water microcosms from the first to the fourth year......Page 56
4. Discussion......Page 57
References......Page 58
2. Material and Methods......Page 59
3.2 DNA Purification and PCR:......Page 60
4. Discussion......Page 61
References......Page 62
1. Introduction......Page 63
3. Results and Discussion......Page 64
3.1 Extracts from auto-hydrolysis of wood......Page 65
3.2 Extracts from acid hydrolysis of wood......Page 66
References......Page 67
2. Material and methods......Page 68
3. Results......Page 69
4. Discussion......Page 70
5. References......Page 71
2.3 Fungal cultures......Page 72
3. Results and discussion......Page 73
4. Conclusion......Page 74
References......Page 75
DNA Extraction......Page 76
Results and Discussion......Page 77
References......Page 79
2. Materials and Methods......Page 81
3.2 Respiratory chain......Page 83
References......Page 85
2.1 Compost preparation and characterization......Page 86
3.1 Compost characteristics......Page 87
3.2 Acification experiment. Isolation and identification of selected strains......Page 88
References......Page 89
Materials and methods......Page 91
Results and discussion......Page 92
References......Page 93
Introduction......Page 95
Results and Discussion......Page 96
References......Page 99
2.1. Bacterial isolates and antigen preparation......Page 100
2.2. PAb production......Page 101
References......Page 104
2.1 Bacterial strain and water microcosms......Page 105
3.2 Pathogenicity of E. amylovora in rain water at low temperatures......Page 106
4. Discussion......Page 107
References......Page 108
1. Introduction......Page 109
3.1 Selection of copper sulphate concentration for adding to KB medium......Page 110
3.4 Plating efficiency of E. amylovora on KBCu medium in vivo......Page 111
References......Page 112
1. Introduction......Page 114
2.5. Dehydrogenase activity......Page 115
3.3. Dehydrogenase activity......Page 116
References......Page 117
Introduction......Page 119
Materials and methods......Page 120
Results......Page 121
References......Page 125
B. Tubers and sawing......Page 126
A. Population of R.solanacearum in potato rhizosphere soil......Page 127
B. Severity of potato bacterial wilt......Page 128
Reference......Page 129
2.1 Soil sampling and analysis......Page 131
3. Results and Discussion......Page 132
References......Page 134
Analytical and Imaging Techniques. Microscopy......Page 136
1. Introduction......Page 138
3.1 Biochemical composition of cell walls of Mucoralean fungi......Page 139
References......Page 141
1. Introduction......Page 143
3.1 Comparative analysis of different microbial techniques of quantification......Page 144
3.2 Correlations between microbial population using by different quantification techniques......Page 145
References......Page 146
1. Introduction......Page 148
2.3.3 Li procedure......Page 149
3.1 Comparison of the analytical procedures for apple juices......Page 150
References......Page 151
2.2 Voltammetric measurements......Page 153
3. Results and discussion......Page 154
References......Page 156
2.2 Absorption spectra and oxygen consumption measurement......Page 157
3.3 Specific wild type and mutants morphology......Page 158
References......Page 161
2.1 Collection of samples......Page 162
3. Results and discussion......Page 163
4. Conclusions......Page 165
References......Page 166
Environmental, Marine, Aquatic Microbiology. Geomicrobiology......Page 168
2.3 Isolation and morphological characterization......Page 170
3. Results and discussion......Page 171
References......Page 172
2. Materials and Methods......Page 174
3. Results and Discussion......Page 175
References......Page 178
Materials and method......Page 179
Discussion......Page 180
References......Page 181
2. Materials and Methods......Page 183
5. Conclusion......Page 185
References......Page 186
1. Introduction......Page 187
3.1. Growth and degradation of hydroxyl substituted phenols by Trichosporon cutaneum R57......Page 188
3.2. Growth and degradation of 2,6 dinitrophenol, α-methylstyrene and acetophenone by Trichosporon
cutaneum R57......Page 190
References......Page 191
1. Introduction......Page 193
3. Results and discussion......Page 194
4. Conclusions......Page 195
References......Page 196
1. Introduction......Page 197
2.4. Heavy Metals Removal and Determination......Page 198
3.2. Zinc biosorption by P. chrysosporium and C. elegans......Page 199
References......Page 200
2.1 Microorganisms and Culture Conditions......Page 201
3. Results and Discussion......Page 202
References......Page 204
2. Materials and Methods......Page 205
3. Results and Discussion......Page 206
References......Page 209
2.1 Sampling......Page 210
3.1 Analysis of bioaerosol comparing the bioaerosol samples at 3m and 600m......Page 211
3.2 Physiological and phylogenetic analysis of bioaerosol in the bioaerosol sample at 600m......Page 212
References......Page 213
1.2. Disinfection methods against Legionella......Page 215
1.3. Persistence of Legionella after treatments......Page 216
2.1 Material and methods......Page 217
2.2 Results and discussion......Page 218
References......Page 219
2.1. Microorganism......Page 220
3. Results and discussion......Page 221
References......Page 224
1. Introduction......Page 225
2.3.1. Qualitative methods.......Page 226
3.3. Enzymatic activities by APIZYM system......Page 227
References......Page 228
2.1 Sludge samples......Page 230
2.2 Molecular techniques......Page 231
3.2.Detection of ppk gene in activated sludge......Page 232
4. Discussion......Page 233
References......Page 235
Introduction......Page 236
Materials and Methods......Page 237
References......Page 239
2.1. Microorganism......Page 240
2.3.5. Cellular Viability......Page 241
3. Results......Page 242
5. Conclusions......Page 243
References......Page 244
1. Introduction......Page 245
3. Results and discussion......Page 246
References......Page 248
1. Introduction......Page 249
3. Results and discussion......Page 250
References......Page 252
2. Materials and Methods......Page 253
3. Results......Page 254
4. Discussion......Page 255
References......Page 256
2.2 Reactors operation......Page 257
3. Results and Discussion......Page 258
References......Page 260
2.1. Microorganisms and growth conditions......Page 262
3. Results......Page 263
References......Page 265
2.2 Collect and isolation......Page 266
2.6 Factorial planning......Page 267
3. Results and Discussion......Page 268
References......Page 270
1. Introduction......Page 271
2.5 Detection of Enzymatic Activity......Page 272
3. Results and Discussion......Page 273
4. Conclusions......Page 274
References......Page 275
1. Introduction......Page 276
3. Results and Discussion......Page 277
References......Page 279
2. Materials and methods......Page 281
3. Results and discussion......Page 282
References......Page 284
1. Introduction......Page 285
2. In situ biofilm monitoring by ATR-FTIR spectroscopy......Page 286
3. Response of the 6-h P. fluoresens nascent biofilm to changes in dissolved organic carbon level......Page 287
4. Conclusion......Page 288
References......Page 289
2.2 Analytical methods......Page 290
3.2 Biochemical composition......Page 291
References......Page 294
2.2 Analysis of ammonia, nitrite and nitrate......Page 295
3.2 The species of purified bacteria in the anammox sludge......Page 296
4. Conclusion......Page 297
References......Page 298
Materials and Methods......Page 299
Results and Discussion......Page 300
References......Page 302
2. Material and Methods......Page 303
3.1 Mass balance......Page 304
3.2 Microbial diversity......Page 305
References......Page 306
1. Introduction......Page 308
3. Results and Discussion......Page 309
References......Page 311
1. Introduction......Page 312
2. Materials and Methods......Page 313
3. Results and Discussion......Page 314
References......Page 316
Materials and Methods......Page 317
Conclusion......Page 318
References......Page 320
2.1 Interactions types between bacteria and protozoa......Page 321
3. Conclusions and future prospects......Page 323
References......Page 324
2.1 Sights......Page 325
2.3 Viability check......Page 326
3. Results......Page 327
References......Page 329
3. Results and Discussion......Page 330
References......Page 333
1. Introduction......Page 334
3.1 System performance......Page 335
3.2 Microbial characterization......Page 336
4. Conclusions......Page 337
5. References......Page 338
1. Introduction......Page 339
3.1 Electricity Generated From Wastewater......Page 340
3.1.3 Generation of electricity in Carbon filter sample......Page 341
4.3 Physical factors......Page 342
References......Page 343
1. Introduction......Page 344
4. MFC Tests......Page 345
5. Calculation and Analysis......Page 346
6.2.1 In series and In parallel......Page 347
8. Conclusion......Page 348
References......Page 349
2.1 Sampling......Page 350
3.1 Seasonal variation in Lake Kahokugata......Page 351
3.3 Classification and identification of DMAA-degrading bacteria using 16S rDNA information......Page 352
References......Page 353
2. Material and Methods......Page 355
3. Results......Page 356
4. Discussion......Page 357
References......Page 358
2.1 DNA extraction, amplification and DGGE fingerprinting......Page 359
3.2 Clone library analysis......Page 360
3.4 General discussions......Page 361
References......Page 362
1. Introduction......Page 364
2. Methodology......Page 365
3. Results......Page 366
Bibliography......Page 368
2. Material and Methods......Page 369
3. Results and Discussion......Page 370
4. Conclusion......Page 371
References......Page 372
Food Microbiology......Page 374
2.1 Yeast strains and growth conditions......Page 376
4. Conclusions......Page 377
References......Page 379
2.1.2 Sweet potato......Page 380
3.1 Transformation of sweet potato medium by Aspergillus niger AHU7120.......Page 381
3.3 Transformation of 4-OH-PEA with A. niger......Page 382
3.6 Antioxidant activities of substrate, products and related compounds......Page 383
References......Page 384
2.1. Organisms, media and culture conditions......Page 385
3.1 Initial screening and salinity significance......Page 386
3.2 Biocontrol activity: Effect of temperature and influence in OTA production......Page 387
References......Page 388
2.2. Plate assay for killer activity at different pHs......Page 390
3.1. Types of killer yeasts from SW Spain......Page 391
4. Conclusions......Page 393
References......Page 394
2. Biosynthesis of CLA by rumen bacteria......Page 395
3.3. Antiatherogenic activity of CLA......Page 396
References......Page 397
2.1 Chemicals and reagents......Page 399
3.1 Fermentation process......Page 400
3.3 Effect of the baking process on the inner and outer part of bread......Page 401
References......Page 402
2.1 Inoculum Preparation......Page 403
3.1 Effect of bead diameter by fermentation with immobilized S. bayanus EC 1118 and S. carlbergensis TISTR 5345......Page 404
3.2 Stability of immobilized cells......Page 406
4. Conclusions......Page 407
References......Page 408
2.2 Mycological analysis......Page 409
3. Results and discussion......Page 410
References......Page 412
1. Introduction......Page 414
2.3 Sequencing of QRDR region of gyrA gene......Page 415
4. Conclusions......Page 416
References......Page 417
1. Introduction......Page 418
2.1. Sampling......Page 419
3.1. Microbiological analysis......Page 420
3.2. Saccharomyces yeasts characterization......Page 421
References......Page 422
1. Introduction......Page 424
3. Results and discussion......Page 425
References......Page 427
1. Introduction......Page 429
3. Results and Discussion......Page 430
References......Page 432
1. Introduction......Page 433
3.1. Color measurement......Page 434
3.2. Fastness properties......Page 435
References......Page 437
Industrial Microbiology. Future Bioindustries......Page 440
2.1 Advantages of MEOR technique......Page 442
3.2 Selection of the microbial culture......Page 443
3.8 Extraction of oil......Page 444
References......Page 445
2. Characteristics of raw glycerol......Page 446
3. Microorganism mechanisms for glycerol bioconversion......Page 447
4. Advances on glycerol fermentation in Brazil......Page 448
References......Page 449
1. Introduction......Page 451
3. Results......Page 452
4. Discussion......Page 453
References......Page 454
1. Introduction......Page 455
3.1 Enzymatic hydrolysis of botryosphaeran......Page 456
3.2 Enzymatic hydrolyzes of laminarin......Page 457
References......Page 458
Material and methods......Page 459
Conclusion......Page 460
References......Page 461
2.2 Measurement of cell dry weight......Page 462
3. Results and discussion......Page 463
References......Page 464
1. Introduction......Page 465
2.3.3 Hydrophobic interaction chromatography......Page 466
2.9 Substrate specificity......Page 467
3.2 Purification of β-glucosidase......Page 468
3.4 Effect of temperature and pH on β-glucosidase activity and stability......Page 469
3.6 Substrate specificity......Page 470
3.8 Internal amino acid sequence of β-glucosidase by LC-MS/MS......Page 471
References......Page 472
1. Introduction......Page 473
3. Results and disscusion......Page 474
References......Page 475
2.1 Chemicals......Page 477
3.1 Extraction of LPS by classical phenol-chloroform-petroleum ether (PCP) method......Page 478
3.2 Extraction of LPS by scCO2 /Ruggedness Test......Page 479
References......Page 481
1. Introduction......Page 482
2.5 Solid-liquid extraction (“Leaching”)......Page 483
3. Results and discussion......Page 484
References......Page 486
Materials and methods......Page 487
Results and discussion......Page 488
References......Page 491
2.1 Microorganism and culture media......Page 492
3.1 The potential use of xylose as a fermentable sugar......Page 493
3.2.2 Acid-hydrolysates as substrate......Page 494
3.2.3 Auto-hydrolysates as substrate......Page 495
References......Page 496
Medical Microbiology. Pharmaceutical Microbiology......Page 498
Short communication......Page 500
References......Page 502
2.1 Bacterial strains and antimicrobial susceptibility tests......Page 504
2.5 Enterobacterial repetitive consensus (ERIC) PCR......Page 505
3.2 Genotypes of ESBL and isoelectric focusing (IEF)......Page 506
3.3 Characteristics of E. cloacae producing CTX-M-9......Page 507
4. Discussion......Page 508
References......Page 509
1. Introduction......Page 510
3.1 Detection of species by multiplex PCR and clinical data......Page 511
4. Discussion......Page 512
5. References......Page 513
1. Introduction......Page 514
3.1 Minimum Inhibitory Concentration (MIC) of Herbal Extracts and Combination of Herbal Extracts......Page 515
3.2 Antibacterial effect of herbal liquid soap......Page 516
References......Page 517
1. Introduction......Page 518
3. Results and discussion......Page 519
References......Page 520
1. Introduction......Page 522
2.4 Cellular adherence assays......Page 523
3.2 Cellular adherence assays......Page 524
References......Page 525
1. Introduction......Page 527
3. Results......Page 528
References......Page 531
2. Materials and Methods......Page 532
3.3 Effect of doxorubicin on SOD and CAT activities......Page 533
References......Page 535
2.1 Endophytic fungus......Page 536
2.6 Reference antibiotics......Page 537
3. Results and Discussion......Page 538
References......Page 539
1. Introduction......Page 540
2.5 Structural investigations-adsorption of the proteins on montmorillonite [14]......Page 541
3. Results......Page 542
4. Discussion......Page 543
References......Page 544
3.1. Cell adhesion and viral replication......Page 545
5.1. The strategy......Page 546
5.1.3. The evolution of global polio eradication......Page 547
6- Current situation......Page 548
References......Page 549
Methods – Quantitative Models and Bioinformatics in Microbiology......Page 550
2.1. Microorganisms......Page 552
2.4. Quantifications......Page 553
3.2. The pathway of toluene assimilation......Page 554
3.3. The balance between ortho and meta routes......Page 555
References......Page 556
1. Introduction......Page 557
2.4 Multilayer layer perceptron NNs......Page 558
3.1 Multi-layer perceptron NNs......Page 559
References......Page 560
1. Introduction......Page 562
2.4. Determination of the specificity, sensitivity and titre of the IFAS test using different MAbs......Page 563
2.6. Target DNA purification from plant extracts......Page 564
3.1. Characterisation and validation of new MAbs for use in IFAS test......Page 565
3.4. Sensitivity and specificity of detection using quantitative real-time PCR (TaqMan-PCR)......Page 566
3.5. Comparison of detection methods......Page 567
References......Page 568
1. Introduction......Page 569
2.1 Expression and purification of rpreproSP-B......Page 570
3.1 Expression and purification of rprepro-SP-B......Page 571
3.2 Characterization of purified recombinant rpreproSP-B......Page 572
4. Discussion......Page 573
References......Page 574
2. Our knowledge and DNA databases......Page 575
3. Looking into one’s needs......Page 576
4. Future perspectives......Page 578
References......Page 579
Microbial Physiology, Metabolism and Gene Expression......Page 580
2.1 Yeast strains, media and growth conditions......Page 582
3.1 Elements at the KlCYC1 3´-UTR......Page 583
3.4 Stability under hypoxia for KlCYC1, a cytochrome c encoding gene......Page 584
References......Page 585
Introduction......Page 586
Materials and Methods......Page 587
Results and Discussion......Page 588
References......Page 590
1. Introduction......Page 591
3.3. Fragment 7 contains the elements for growth media dependent regulation of KlHIS4......Page 592
References......Page 593
2. Methodology......Page 594
3.2 Genes associated with oxygen response......Page 596
References......Page 597
2. Materials and Methods......Page 598
3.2 Effect of tested redox mediators on AO7 decolourisation in presence of glucose as a co-substrate......Page 599
References......Page 600
1. Introduction......Page 601
2.5 Shake flask expression experiments......Page 602
3. Results......Page 603
4. Discussion......Page 604
References......Page 605
1. Introduction......Page 606
3.1 Analysis of the upstream region of pgx1 and pgx2 genes......Page 607
3.2 Expression of the promoter regions of pgx1 and pgx2 of FORL in S. cerevisiae......Page 608
4. Discussion......Page 609
References......Page 610
Objective......Page 611
Results......Page 612
References......Page 613
1. Introduction......Page 614
3. Results and discussion......Page 615
References......Page 618
1. Introduction......Page 619
2.2. Starvation of DNA precursors induces DSBs......Page 620
2.4. Reversed replication forks prevent lethality......Page 621
3. Concluding remarks......Page 622
References......Page 623
1. Introduction......Page 624
2.5. Analytical determinations......Page 625
3.2. Cotransformation of Chlamydomonas with pSI103 and the constructed vectors pSI104PLK- bkt1 and pSI104-tp-bkt1......Page 626
References......Page 627
2.1 Difference of SigB concentration affects biofilm formation......Page 628
3. Discussion......Page 629
4. References......Page 631
2. Materials and Methods......Page 632
3.1 Structural characterization of two new MT genes in Tetrahymena rostrata......Page 633
3.2 Each isolated T. rostrata MT is included in one Tetrahymena MT subfamily......Page 634
References......Page 635
Microbiology Education......Page 636
2.1 Observation of bacterial morphologies......Page 638
2.2 DNA isolation......Page 640
References......Page 641
2. Methodology......Page 642
3. Results and Discussion......Page 643
3.1 Results and discussion by parameter of analysis......Page 644
4. Further Information......Page 645
Textbooks Used......Page 646
1. Introduction......Page 647
3. Results......Page 648
References......Page 650
Bioremediation......Page 652
2. Materials and methods......Page 654
3.2 Changes of pH and Eh in soil samples amended with different components......Page 655
3.3 Changes of microbial community in contaminated soils during incubation with various amendments......Page 656
References......Page 657
1. Introduction......Page 658
3. Results and Discussion......Page 659
4. References......Page 661
2. Materials and Methods......Page 662
3. Results......Page 663
5. Conclusions......Page 666
References......Page 667
2.1 Chemicals......Page 668
2.6 Evaluation of Lead removal by biomass, dead biomass and exopolysaccharide......Page 669
3.4 Effect of pH, temperature and NaCl concentration on removal rate......Page 670
4. Discussion......Page 671
References......Page 672
1. Background......Page 673
2.4 Ochratoxin A determination......Page 674
3.1 Evolution of OTA level in LAB cultures......Page 675
References......Page 676
2.1 Bacterial growth conditions and sample preparation......Page 677
3 Results and discussion......Page 678
References......Page 680
2.1 Groundwater samples used in this study......Page 681
2.6 Sequencing and phylogenetic analysis......Page 682
3.3 T-RFLP investigations of C23O products......Page 683
4. Discussion......Page 684
References......Page 685
Biosurfactants: Purification, Mass Production, Applications......Page 686
1. Introduction......Page 688
3.1 Bioremediation of aliphatic hydrocarbon contaminated soil......Page 689
3.2 Washing of crude oil contaminated soil......Page 691
References......Page 692
1. Introduction......Page 693
3.1 Biosurfactant production for C. prodigiosum (S. marcencens)......Page 694
3.2. Main effects of the variables used on the surface tension of the surfactant produced by C. prodigiosum (S. marcencens) with 72H of cultivation with and without shake......Page 695
References......Page 697
2.2 Growth conditions......Page 698
3.1 Kinetic growth of Candida lipolytica......Page 699
3.2 Emulsification index......Page 700
3.4 Enzymatic activity......Page 701
References......Page 702
1. Introduction......Page 703
2.4 Determination of SAC activities......Page 704
3.1 Isolation and phylogenetic analysis of bacterial strains......Page 705
References......Page 706
2.1 Microrganism and inoculum preparation......Page 708
3.1 Kinetic growth of Candida glabrata......Page 709
3.3 Properties of the selected bioemulsifier......Page 710
3.4 Bioemulsifier isolation......Page 711
References......Page 712
2.1 Microorganism......Page 713
2.7 Statistical analysis......Page 714
3.1 Factorial designs and growth......Page 715
References......Page 717
2. Materials and Methods......Page 718
3.1 Production of the biosurfactant......Page 719
3.3 Stability of biosurfactant according to the emulsification index......Page 720
3.5 Application of the biosurfactant in the oil removal......Page 721
References......Page 722
Biotechnologically Relevant Enzymes and Proteins......Page 724
Material and Methods......Page 726
Results......Page 728
Discussion......Page 730
Bibliography......Page 731
1. Introduction......Page 732
2.4 Bioconversion of xylose into xylitol......Page 733
3.1 File formats and templates......Page 734
4. Conclusion......Page 735
References......Page 736
2 Materials and methods......Page 737
3 Results......Page 739
References......Page 741
1. Introduction......Page 742
3.1 Dyebath exhaustions......Page 743
3.4 Colorimetric measurements......Page 744
References......Page 745
2. Materials and Methods......Page 746
3.1 Cholesterol oxidase induction in cells of strain GK1......Page 747
3.3.2 Behavior throughout gel filtration and SDS-PAGE......Page 748
4.1. Cholesterol oxidase induction......Page 750
4.2 Occurrence of cholesterol oxidase forms......Page 751
References......Page 752
Microfactories – Microbial Production of Chemicals and Pharmaceuticals......Page 754
2.2. Analytical methods......Page 756
3.1. Selection of CA producing mutants......Page 757
3.2. Effect of dissolved oxygen and shear conditions on CA production......Page 758
3.5. Results using endophytic microorganisms and antibiosis......Page 759
References......Page 760
2.2 Chitin and Chitosan Production, Extraction and Analysis......Page 761
3.2 Growth, Chitin and Chitosan Production......Page 762
3.3 Chitosan analysis......Page 764
References......Page 765
1. Introduction......Page 766
2. Materials and methods......Page 767
3. Results and discussion......Page 768
References......Page 770
2. Material and methods......Page 771
3. Results and discussion......Page 772
References......Page 776
2.1 Materials......Page 777
3. Experimental Procedure......Page 778
4. Results and Discussion......Page 779
References......Page 781
2.1. Production of clavulanic acid broth......Page 782
2.6. Definition and measurement of pseudo steady-state permeate flux (Jps) and fouling index (I)......Page 783
3.2. Experiments with microfiltration membrane......Page 784
4. Conclusions......Page 787
References......Page 788