Current Research in Protein Chemistry. Techniques, Structure and Function

Content: 
Front Matter, Page iii
Copyright, Page iv
Foreword, Page xiii
Preface, Page xv
Dedication to Emil T. Kaiser and Irving S. Sigal, Pages xvii-xxv
Acknowledgments, Page xxvii
1 - A RAPID AND NOVEL MEANS OF PROTEIN HYDROLYSIS BY MICROWAVE IRRADIATION USING TEFLON-PYREX TUBES, Pages 3-10
2 - Analysis of Amino Acids by High Performance Capillary Electrophoresis, Pages 11-22
3 - An Evaluation of Microwave Heating for the Vapor Phase Hydrolysis of Proteins: I. Comparison to Vapor Phase Hydrolysis for 24 Hours, Pages 23-36
4 - PERFORMANCE EVALUATION AUTOMATIC HYDROLYSIS AND PTC AMINO ACID ANALYSIS, Pages 37-48
5 - A COLLABORATIVE AMINO ACID ANALYSIS STUDY FROM THE ASSOCIATION OF BIOMOLECULAR RESOURCE FACILITIES, Pages 49-61
6 - RECENT DEVELOPMENTS IN SOLID PHASE PEPTIDE SYNTHESIS USING THE 9-FLUORENYLMETHYOXYCARBONYL (FMOC) PROTECTING GROUP STRATEGY, Pages 63-77
7 - RELATIONSHIPS OF PARAMECIUM AND ENDOCRINE SECRETORY PROTEINS, Pages 79-85
8 - Quantitative Electrotransfer of Proteins From Polyacrylamide Gels onto PVDF Membranes, Pages 87-93
9 - Selective Isolation of Disulfide-Containing Peptides from Trypsin Digests Using Strong Cation Exchange HPLC, Pages 95-102
10 - ANALYSIS OF PEPTIDES AND PROTEINS BY TANDEM QUADRUPOLE MASS SPECTROMETRY, Pages 105-116
11 - PROTEIN AND OLIGOPEPTIDE SEQUENCE ANALYSIS ON THE TSQ-70 TRIPLE QUADRUPOLE MASS SPECTROMETER, Pages 117-126
12 - Sequence Analysis Of Peptides By Plasma Desorption Mass Spectrometry, Pages 127-138
13 - Study of the Primary Structure of Recombinant Proteins with a Benchtop Gas Chromatograph-Mass Spectrometer-Computer Data System, Pages 139-149
14 - Synthesis and Mass Spectrometry of Phosphotyrosine-Containing Peptides, Pages 151-158
15 - Design, Characterization and Results of ABRF-89SEQ: A Test Sample For Evaluating Protein Sequencer Performance in Protein Microchemistry Core Facilities, Pages 159-166
16 - Extended N-Terminal Protein Sequencing of Clostridium botulinum Neurotoxin Type A Fragments: Continuous Sequence to Seventy Residues Both from Glass-Fiber Filter Using the Gas Phase and Polyvinylidene Difluoride Using the Pulsed Liquid Phase Sequencer, Pages 167-178
17 - PEPTIDE MAPPING AND INTERNAL SEQUENCING OF PROTEINS FROM ACRYLAMIDE GELS, Pages 179-190
18 - New Approaches to Covalent Sequence Analysis, Pages 191-202
19 - ANALYSIS OF BRAIN CONSTITUENTS BY CAPILLARY ELECTROPHORESIS, Pages 203-216
20 - Capillary Electrophoresis Analysis of Species Variations in the Tryptic Maps of Cytochrome C, Pages 217-232
21 - ULTRAFAST PROTEIN ANALYSIS: A POWERFUL TECHNIQUE FOR RECOMBINANT PROTEIN PROCESS MONITORING, Pages 233-244
22 - Separation and Characterization of Proteins in the Range of 1 to 200 kDa with HPEC™, Pages 245-262
23 - Protein Detection During Polyacrylamide Gel Electrophoresis Using High Resolution Dynamic Imaging, Pages 263-269
24 - DESIGN, COMPUTER DERIVED STRUCTURE AND BIOLOGICAL ACTIVITY OF THREE BICYCLIC GONADOTROPIN RELEASING HORMONE (GnRH) ANTAGONISTS, Pages 273-281
25 - THE H-HELIX OF MYOGLOBIN AS A POTENTIAL INDEPENDENT PROTEIN FOLDING DOMAIN, Pages 283-293
26 - Probing the Role of Position 49 in the Folding of the Alpha Subunit of Tryptophan Synthase from Escherichia Coli, Pages 295-300
27 - Tests Of Isolated β–Sheet Formation In A Cyclic Peptide From BPTI, Pages 301-308
28 - Spectroscopic and Chromatographic Studies of Native and Denatured States of T4 Lysozymes, Pages 309-322
29 - Studies of the Alkaline Denaturation of Hemoglobin in an NMR Optical Stopped-Flow Apparatus, Pages 323-329
30 - CONFORMATIONAL STUDIES OF PEPTIDES CORRESPONDING TO THE LDL RECEPTOR CYTOPLASMIC TAIL AND TRANSMEMBRANE DOMAIN, Pages 331-338
31 - Biochemical and Biophysical Comparison of Bacterial DnaK and Mammalian hsc73, Two Members of an Ancient Stress Protein Family, Pages 339-347
32 - The Crystal Structure of Human Interleukin-1α, Pages 351-358
33 - Crystal Structure of a Protein-Toxin Solved from a Predicted Model, Pages 359-366
34 - Segmental Accommodation: A Novel Conformational Change Induced Upon Ligand Binding by Thymidylate Synthase, Pages 367-382
35 - Subunit Interactions and the Function of Tumor Necrosis Factor, Pages 383-394
36 - STRUCTURE PREDICTION METHODS FOR MEMBRANE PROTEINS: COMPARISON WITH THE X-RAY STRUCTURE OF THE R. VIRIDIS PHOTOSYNTHETIC REACTION CENTRE, Pages 395-404
37 - THE SEQUENCE ATTRIBUTES METHOD FOR DETERMINING CORRELATIONS BETWEEN AMINO ACID SEQUENCE AND PROTEIN SECONDARY STRUCTURE, Pages 405-415
38 - Acid-Denatured States of Proteins, Pages 417-424
39 - ESTIMATION OF PROTEIN STABILITY COMPARISON OF CALORIMETRIC AND DENATURANT STUDIES, Pages 425-434
40 - ENGINEERING OF MULTIPLE DISULFIDE BONDS DRAMATICALLY STABILIZES T4 LYSOZYME, Pages 435-447
41 - E. coli Thioredoxin Stability Is Greatly Enhanced by Substitution of Aspartic Acid 26 by Alanine, Pages 449-456
42 - MEASUREMENTS OF DISULFIDE BOND STABILITIES IN PROTEIN FOLDING INTERMEDIATES, Pages 457-466
43 - SOLUBILITY OF RECOMBINANT PROTEINS EXPRESSED IN ESCHERICHIA COLI, Pages 467-478
44 - MULTIPLE DETERMINANTS FOR SUBSTRATE SPECIFICITIES IN CYTOCHROME P450 ISOZYMES, Pages 481-488
45 - Catalysis by Chimeric Proteins: Conversion of a β-Iactamase to a D,D-Carboxypeptidase, Pages 489-497
46 - Molecular Mapping of Immunogenic Determinants of Human CD4 Using Chimeric Interspecies Molecules and Anti-CD4 Antibodies, Pages 499-508
47 - EETI II (Ecballium elaterium Trypsin Inhibitor II) as a Vehicle for New Functions, Pages 509-519
48 - Comparison of the Dimerization, Stability and Inhibitor Binding Between HIV-1 Protease Wild Type and Active Site Mutant, Pages 521-528
49 - STRUCTURE / FUNCTION ANALYSIS OF IL-2-TOXIN: FRAGMENT B REQUIREMENTS FOR THE TRANSLOCATION OF FRAGMENT A INTO THE CYTOSOL OF TARGET CELLS, Pages 529-540
50 - ALTERATION OF THE SITE OF ADENYLYLATION OF GLUTAMINE SYNTHETASE BY SITE-DIRECTED MUTAGENESIS, Pages 541-548
51 - Three-Dimensional Protein Structure Prediction Workshop: Overview and Summary, Pages 551-556
52 - PROTEIN STRUCTURE PREDICTION, Pages 557-565
53 - Knowledge-Based Protein Modelling: Human Plasma Kallikrein and Human Neutrophil Defensin, Pages 567-574
54 - Application of a Knowledge Based Strategy to the Prediction of Protein Tertiary Structure, Pages 575-582
55 - Structure Determination of Haloalkane Dehalogenase, Pages 583-587
Index, Pages 589-606