Crystallography in Molecular Biology

Front Matter....Pages i-x
Front Matter....Pages 1-1
Crystallization of Integral Membrane Proteins....Pages 3-14
Crystallization of Protein and Nucleic Acids: A Survey of Methods and Importance of the Purity of the Macromolecules....Pages 15-26
Synchrotron Radiation and Macromolecular Crystallography....Pages 27-44
Data Collection....Pages 45-59
Front Matter....Pages 61-61
Density Modification Methods....Pages 63-79
Anomalous Scattering in Macromolecular Structure Analysis....Pages 81-87
Single Molecule Electron Crystallography....Pages 89-99
Structure Analysis of Bacteriorhodopsin by Electron Crystallography....Pages 101-115
Neutron Diffraction : Contribution to High and Low Resolution Crystallography....Pages 117-121
Front Matter....Pages 123-123
Electron Density Fitting with Computer Graphics: A Review and a Glimpse....Pages 125-130
Computer Graphics in the Study of Macromolecular Interactions....Pages 131-139
Prediction of Protein Structure from Amino Acid Sequence....Pages 141-149
Front Matter....Pages 151-151
X-Ray Analysis of Polypeptide Hormones at ≦1Å Resolution: Anisotropic Thermal Motion and Secondary Structure of Pancreatic Polypeptide and Deamino-Oxytocin....Pages 153-165
Water at Biomolecule Interfaces....Pages 167-177
The Water Structure in 2Zn Insulin Crystals....Pages 179-192
The Structure of Water Around a Macrocyclic Receptor: A Monte Carlo Study of the Hydration of 18-Crown-6 in Different Conformations....Pages 193-195
Simulating Protein Dynamics in Solution: Bovine Pancreatic Trypsin Inhibitor....Pages 197-205
Front Matter....Pages 207-207
Rational Design of DNA Minor Groove-Binding Anti-Tumor Drugs....Pages 209-221
Protein Crystallography and Drug Design....Pages 223-228
Enzyme Mechanism: What X-Ray Crystallography can(not) Tell Us....Pages 229-240
Front Matter....Pages 207-207
The Role of Separate Domains and of Individual Amino-Acids in Enzyme Catalysis, Studied by Site-Directed Mutagenesis....Pages 241-249
Selected and Directed Mutants of T4 Phage Lysozyme....Pages 251-260
Front Matter....Pages 261-261
The Structure of a Human Common Cold Virus (Rhinovirus 14) and its Functional Relations to Other Picornaviruses....Pages 263-280
The Structure of Poliovirus at 2.9 Å Resolution: Crystallographic Methods and Biological Implications....Pages 281-292
The Structure of Cowpea Mosaic Virus at 3.5 Å Resolution....Pages 293-308
Adenovirus Architecture....Pages 309-316
Front Matter....Pages 317-317
A Bacteriophage Repressor/Operator Complex at 7 Å Resolution....Pages 319-325
Refined Structure of DNase 1 at 2 Å Resolution and Model for the Interaction with DNA....Pages 327-335
Ribonucleases A and T 1 Comparable Mechanisms of RNA Cleavage with Different Active Site Geometries....Pages 337-344
Structural Studies of ECO RV Endonuclease and of its Complexes with Short DNA Fragments....Pages 345-352
Errors in DNA Helices: G.T Mismatches in the A, B and Z Forms of DNA....Pages 353-355
Front Matter....Pages 357-357
Relation between Functional Loop Regions and Intron Positions in α/ß Domains....Pages 359-364
The Three-Dimensional Structure of Antibodies....Pages 365-371
The 3-Dimensional Structures of Influenza Virus Neuraminidase and an Antineuraminidase Fab Fragment....Pages 373-383
Periplasmic Binding Proteins: Structures and New Understanding of Protein-Ligand Interactions....Pages 385-394
Crystal Structure of Thaumatin I, a Sweet Taste Receptor Binding Protein....Pages 395-402
Structures of Pyruvoyl-Dependent Histidine Decarboxylase and Mutant-3 Prohistidine Decarboxylase from Lactobacillus 30A ....Pages 403-411
State of X-Ray Structure Determination of Ascorbate Oxidase from Green Zucchini Squash....Pages 413-416
X-Ray Structure of the Light-Harvesting Biliprotein C-Phycocyanin from M. laminosus ....Pages 417-420
The Crystal Structure of the Photosynthetic Reaction Center from Rhodopseudomonas Viridis ....Pages 421-423
Front Matter....Pages 357-357
Structure and Function of Some Electron Transfer-Proteins and-Complexes Monoheme C-Type-and Multiheme C 3 -Cytochromes....Pages 425-437
Back Matter....Pages 439-443