Correlative Light and Electron MIcroscopy

Content: 
Series Editors
Page ii

Front Matter
Page iii

Copyright
Page iv

Contributors
Pages xi-xv

Preface
Pages xvii-xix
Thomas Müller-Reichert, Paul Verkade

Chapter 1 - Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
Pages 1-20
K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson, F. Braet

Chapter 2 - Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy
Pages 21-35
Roman S. Polishchuk, Elena V. Polishchuk, Alberto Luini

Chapter 3 - Correlative Fluorescence and Transmission Electron Microscopy in Tissues
Pages 37-57
Toshihiro Takizawa, John M. Robinson

Chapter 4 - Correlative Light and Electron Microscopy in Parasite Research
Pages 59-73
Céline Loussert, Claire-Lise Forestier, Bruno M. Humbel

Chapter 5 - Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
Pages 75-93
Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, Thomas Kurth

Chapter 6 - 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
Pages 95-115
Katia Cortese, Giuseppe Vicidomini, Maria Cristina Gagliani, Patrizia Boccacci, Alberto Diaspro, Carlo Tacchetti

Chapter 7 - Correlative Light and Electron Microscopy of GFP
Pages 117-138
Markus Grabenbauer

Chapter 8 - Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
Pages 139-155
Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu, Gina E. Sosinsky

Chapter 9 - Correlated Light Microscopy and Electron Microscopy
Pages 157-173
Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan, Ben N.G. Giepmans

Chapter 10 - Capturing Endocytic Segregation Events with HPF-CLEM
Pages 175-201
Edward Brown, Jan Van Weering, Thom Sharp, Judith Mantell, Paul Verkade

Chapter 11 - Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
Pages 203-222
Irina Kolotuev, Daniel J. Bumbarger, Michel Labouesse, Yannick Schwab

Chapter 12 - Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo
Pages 223-234
Ina Woog, Silke White, Mandy Büchner, Martin Srayko, Thomas Müller-Reichert

Chapter 13 - Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
Pages 235-257
Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen, John A.G. Briggs

Chapter 14 - Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining
Pages 259-281
Alexander Rigort, Elizabeth Villa, Felix J.B. Bäuerlein, Benjamin D. Engel, Jürgen M. Plitzko

Chapter 15 - Visualizing Proteins in Electron Micrographs at Nanometer Resolution
Pages 283-306
Shigeki Watanabe, Erik M. Jorgensen

Chapter 16 - Atmospheric Scanning Electron Microscope for Correlative Microscopy
Pages 307-324
Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood, Peter J. O’Toole

Chapter 17 - Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures
Pages 325-356
Miriam S. Lucas, Maja Günthert, Philippe Gasser, Falk Lucas, Roger Wepf

Chapter 18 - Correlative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
Pages 357-382
Andrew J. Bushby, Giovanni Mariggi, Hannah E.J. Armer, Lucy M. Collinson

Index
Pages 383-393

Volumes in Series
Pages 395-404

Colour Plate
Pages 1-40