Corona- and Related Viruses: Current Concepts in Molecular Biology and Pathogenesis

Front Matter....Pages i-xvii
Front Matter....Pages N1-N1
Genetic Resistance to Coronavirus Infection....Pages 1-11
Factors Controlling Coronavirus Infections and Disease of the Central Nervous System....Pages 13-22
Characterization of the S Protein of Enterotropic Murine Coronavirus Strain-Y....Pages 23-28
Biological and Molecular Differentiation Between Coronaviruses Associated with Neonatal Calf Diarrhoea and Winter Dysentery in Adult Cattle....Pages 29-33
Molecular Differentiation of Transmissible Gastroenteritis Virus and Porcine Respiratory Coronavirus Strains....Pages 35-41
Organ Specific Endothelial Cell Heterogeneity Influences Differential Replication and Cytopathogenicity of MHV-3 and MHV-4....Pages 43-50
Genomic Regions Associated with Neurotropism Identified in MHV by RNA-RNA Recombination....Pages 51-56
Involvement of Microtubules and the Microtubule-Associated Protein TAU in Trafficking of JHM Virus and Components within Neurons....Pages 57-61
Evolution and Persistence Mechanisms of Mouse Hepatitis Virus....Pages 63-71
Spread of MHV-JHM from Nasal Cavity to White Matter of Spinal Cord....Pages 73-78
In Vitro Interaction of Coronaviruses with Primate and Human Brain Microvascular Endothelial Cells....Pages 79-88
Treatment of Resistant A/J Mice with Methylprednisolone (MP) Results in Loss of Resistance to Murine Hepatitis Strain 3 (MHV-3) and Induction of Macrophage Procoagulant Activity (PCA)....Pages 89-94
Ultrastructural Characteristics and Morphogenesis of Porcine Reproductive and Respiratory Syndrome Virus Propagated in the Highly Permissive MARC-145 Cell Clone....Pages 95-98
Antigenic and Genomic Variations Among Cytopathic and Non-Cytopathic Strains of Bovine Enteric Coronavirus....Pages 99-101
Fragmentation and Rearrangement of the Golgi Apparatus During MHV Infection of L-2 Cells....Pages 103-104
Severe Combined Immunodeficiency (SCID) Mouse Hepatitis Experimentally Induced with Low Virulence Mouse Hepatitis Virus....Pages 105-107
Mouse Hepatitis Virus-Specific CD8+ Cytotoxic T Lymphocytes Induce Apoptosis in Their Target Cells....Pages 109-111
Echocardiographic Changes Following Rabbit Coronavirus Infection....Pages 113-115
Neuronal Spread of Swine Hemagglutinating Encephalomyelitis Virus (HEV) 67N Strain in 4-Week-Old Rats....Pages 117-119
Characterization of Human T Cell Clones Specific for Coronavirus 229E....Pages 121-129
Front Matter....Pages N1-N1
Studies on the in Vitro and in Vivo Host Range of Porcine Epidemic Diarrhoea Virus....Pages 131-133
Coronaviruses in Polarized Epithelial Cells....Pages 135-138
Isolation and Experimental Oral Transmission in Pigs of a Porcine Reproductive and Respiratory Syndrome Virus Isolate....Pages 139-144
Front Matter....Pages N2-N2
The Protective Role of Cytotoxic T Cells and Interferon against Coronavirus Invasion of the Brain....Pages 145-149
MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP ....Pages 151-157
Demyelination Induced by Murine Coronavirus JHM Infection of Congenitally Immunodeficient Mice....Pages 159-163
Induction of a Protective Immune Response to Murine Coronavirus with Non-Internal Image Anti-Idiotypic Antibodies....Pages 165-172
Transcription and Translation of Proinflammatory Cytokines Following JHMV Infection....Pages 173-178
Pathology of MHV-A59 Infection in ß2 Microglobulin Negative Mice....Pages 179-181
Primary Murine Coronavirus Infection in Mice....Pages 183-184
Neurovirulence for Rats of the JHMV Variants Escaped from Neutralization with the S1-Specific Monoclonal Antibodies....Pages 185-187
T Cell Immunodeficiency Involved in Pathogenicity of Attenuated MHV3 Mutants....Pages 189-191
Impairment of Bone Marrow Pre-B and B Cells in MHV3 Chronically-Infected Mice....Pages 193-195
Front Matter....Pages N3-N3
Development of Protection against Coronavirus Induced Diseases....Pages 197-211
Production and Immunogenicity of Multiple Antigenic Peptide (MAP) Constructs Derived from the S1 Glycoprotein of Infectious Bronchitis Virus (IBV)....Pages 213-219
Protection of Cats from Infectious Peritonitis by Vaccination with a Recombinant Raccoon Poxvirus Expressing the Nucleocapsid Gene of Feline Infectious Peritonitis Virus....Pages 221-228
Efficacy of an Inactivated Vaccine against Clinical Disease Caused by Canine Coronavirus....Pages 229-234
Cloning and Expression of FECV Spike Gene in Vaccinia Virus....Pages 235-241
The Use of Arms PCR and RFLP Analysis in Identifying Genetic Profiles of Virulent, Attenuated or Vaccine Strains of TGEV and PRCV....Pages 243-250
Front Matter....Pages N4-N4
Intracellular Localization of Polypeptides Encoded in Mouse Hepatitis Virus Open Reading Frame 1A....Pages 251-258
Front Matter....Pages N4-N4
Bovine Coronavirus Nucleocapsid Protein Processing and Assembly....Pages 259-263
Expression and Immunogenicity of the Spike Glycoprotein of Porcine Respiratory Coronavirus Encoded in the E3 Region of Adenovirus....Pages 265-270
Characterization of Structural Proteins of Lelystad Virus....Pages 271-276
Structural Gene Analysis of a Quebec Reference Strain of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)....Pages 277-281
Site Directed Mutagenesis of the Murine Coronavirus Spike Protein....Pages 283-286
Identification of Proteins Specified by Porcine Epidemic Diarrhoea Virus....Pages 287-290
Coexpression and Association of the Spike Protein and the Membrane Protein of Mouse Hepatitis Virus....Pages 291-297
Functional Domains in the Spike Protein of Transmissible Gastroenteritis Virus....Pages 299-304
Recombinant Expression of the TGEV Membrane Glycoprotein M....Pages 305-310
Translation of the MHV sM Protein is Mediated by the Internal Entry of Ribosomes on mRNA 5....Pages 311-315
Identification and Characterization of the Porcine Reproductive and Respiratory Virus ORFS 7, 5 and 4 Products....Pages 317-320
Structural Proteins of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)....Pages 321-323
Front Matter....Pages N5-N5
Cellular Receptors for Transmissible Gastroenteritis Virus on Porcine Enterocytes....Pages 325-329
Overexpression of the MHV Receptor....Pages 331-336
Multiple Receptor-Dependent Steps Determine the Species Specificity of HCV-229E Infection....Pages 337-343
Characterization of a New Gene that Encodes a Functional MHV Receptor and Progress in the Identification of the Virus-Binding Site(s)....Pages 345-350
MHVR-Independent Cell-Cell Spread of Mouse Hepatitis Virus Infection Requires Neutral pH Fusion....Pages 351-357
Localization of Neutralizing Epitopes and Receptor-Binding Site in Murine Coronavirus Spike Protein....Pages 359-365
Analysis of the Sialic Acid-Binding Activity of Transmissible Gastroenteritis Virus....Pages 367-370
Analysis of Cellular Receptors for Human Coronavirus OC43....Pages 371-374
Front Matter....Pages N5-N5
Polarized Entry of Bovine Coronavirus in Epithelial Cells....Pages 375-378
Overexpression of TGEV Cell Receptor Impairs the Production of Virus Particles....Pages 379-385
Front Matter....Pages N6-N6
Identification of 120 kD and 30 kD Receptors for Human Coronavirus OC43 in Cell Membrane Preparations from Newborn Mouse Brain....Pages 387-390
Inhibition of Coronavirus MHV-A59 Replication by Proteinase Inhibitors....Pages 391-397
Inhibition of Viral Multiplication in Acute and Chronic Stages of Infection by Ribozymes Targeted against the Polymerase Gene of Mouse Hepatitis Virus....Pages 399-404
Identification of a Trypsin-Like Serine Proteinase Domain Encoded by ORF la of the Coronavirus IBV....Pages 405-411
Involvement of Viral and Cellular Factors in Processing of Polyprotein Encoded by ORF1a of the Coronavirus IBV....Pages 413-421
Characterization of the Leader Papain-Like Protease of MHV-A59....Pages 423-430
Proteolytic Processing of the MHV Polymerase Polyprotein....Pages 431-435
Zinc-Binding of the Cysteine-Rich Domain Encoded in the Open Reading Frame 1B of the RNA Polymerase Gene of Coronavirus....Pages 437-442
Proteolytic Processing of the Arterivirus Replicase....Pages 443-451
Identification and Analysis of MHV-A59 P28 Cleavage Site....Pages 453-458
Complete Genomic Sequence of the Transmissible Gastroenteritis Virus....Pages 459-461
Front Matter....Pages N7-N7
Transcription, Replication, Recombination, and Engineering of Coronavirus Genes....Pages 463-471
Analysis of Coronavirus Transcription Regulation....Pages 473-478
Expression of Murine Coronavirus Genes 1 and 7 is Sufficient for Viral RNA Synthesis....Pages 479-484
Dephosphorylation of the Nucleocapsid Protein of Inoculum JHMV may be Essential for Initiating Replication....Pages 485-489
Evidence that MHV Subgenomic Negative Strands are Functional Templates....Pages 491-497
Coronaviruses use Discontinuous Extension for Synthesis of Subgenome-Length Negative Strands....Pages 499-506
Regulation of Transcription of Coronaviruses....Pages 507-510
Front Matter....Pages N7-N7
Evidence for a Pseudoknot in the 3′ Untranslated Region of the Bovine Coronavirus Genome....Pages 511-514
Regulation of Coronavirus RNA Transcription is Likely Mediated by Protein-RNA Interactions....Pages 515-521
Interactions between the IBV Nucleocapsid Protein and RNA Sequences Specific for the 3′ end of the Genome....Pages 523-528
Characterization of the Transmissible Gastroenteritis Virus (TGEV) Transcription Initiation Sequence....Pages 529-535
In Vivo and in Vitro Transcription of Small mRNAs Containing a Leader Sequence from Mouse Hepatitis Virus Strain JHM....Pages 537-539
PEDV Leader Sequence and Junction Sites....Pages 541-542
Front Matter....Pages N8-N8
Mutagenesis of the Genome of Mouse Hepatitis Virus by Targeted RNA Recombination....Pages 543-549
First Experimental Evidence of Recombination in Infectious Bronchitis Virus....Pages 551-556
Molecular Bases of Tropism in the PUR46 Cluster of Transmissible Gastroenteritis Coronaviruses....Pages 557-562
Generation of a Defective RNA of Avian Coronavirus Infectious Bronchitis Virus (IBV)....Pages 563-569
High Recombination and Mutation Rates in Mouse Hepatitis Virus Suggest that Coronaviruses may be Potentially Important Emerging Viruses....Pages 571-576
Hepatitis Mutants of Mouse Hepatitis Virus Strain A59....Pages 577-582
Structure and Encapsidation of Transmissible Gastroenteritis Coronavirus (TGEV) Defective Interfering Genomes....Pages 583-589
Mutations Associated with Viral Sequences Isolated from Mice Persistently Infected with MHV-JHM....Pages 591-597
Back Matter....Pages 599-615