Collagen: Methods and Protocols

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
 Cloning and Mutagenesis Strategies for Large Collagens  (Olivier Bornert, Alexander Nyström)....Pages 3-15
 Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen  (Venkat Raghavan Krishnaswamy)....Pages 17-22
 Production and Characterization of Chemically Cross-Linked Collagen Scaffolds  (Ignacio Sallent, Héctor Capella-Monsonís, Dimitrios I. Zeugolis)....Pages 23-38
 A Biomimetic Model for Mineralization of Type-I Collagen Fibrils  (Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang)....Pages 39-54
Front Matter ....Pages 55-55
 Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy  (Wing Ying Chow)....Pages 57-77
 Measurement of Collagen Cross-Links from Tissue Samples by Mass Spectrometry  (Amar Joshi, Amna Zahoor, Alberto Buson)....Pages 79-93
 Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes  (Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, Matthew D. Shoulders)....Pages 95-114
 Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics  (Elizabeta Madzharova, Fabio Sabino, Ulrich auf dem Keller)....Pages 115-126
Front Matter ....Pages 127-127
 Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy  (Elee Shimshoni, Irit Sagi)....Pages 129-133
 In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology  (Yang Li, S. Michael Yu)....Pages 135-144
 Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics  (Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, Jean-Marc Allain et al.)....Pages 145-155
 Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix  (Uwe Hansen)....Pages 157-166
Front Matter ....Pages 167-167
 Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans  (Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, Collin Y. Ewald)....Pages 169-188
 Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis  (Efrat Kessler, Eyal Hassoun)....Pages 189-201
 Tumorigenic Interplay Between Macrophages and Collagenous Matrix in the Tumor Microenvironment  (Chen Varol)....Pages 203-220
 FACS Analysis of Col1α Protein Levels in Primary Fibroblasts  (Noam Cohen, Neta Erez)....Pages 221-228
 Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors  (Gregg B. Fields)....Pages 229-252
Back Matter ....Pages 253-254