Chromatin and Chromosomal Protein Research. IV

Content: 
Series Editor
Page ii

Edited by
Page iii

Copyright page
Page iv

List of Contributors
Pages xv-xvii

Preface
Page xix
Gary S. Stein, Janet L. Stein, Lewis J. Kleinsmith

Chapter 1. Isolation and Assay of Eukaryotic DNA-Dependent RNA Polymerases Original Research Article
Pages 1-26
Pablo Valenzuela, Graeme I. Bell, Fanyela Weinberg, William J. Rutter

Chapter 2. Isolation and Characterization of DNA Polymerases from Eukaryotic Cells Original Research Article
Pages 27-42
Dipak K. Dube, C Travaglini, C Loeb

Chapter 3. Methods for Assessment of DNase Activity Original Research Article
Pages 43-49
Muriel W. Lambert, George P. Studzinski

Chapter 4. Assessment of Acetylation in Nonhistone Chromosomal Proteins Original Research Article
Pages 51-58
C.C. Liew

Chapter 5. Purification and Characterization of Protein Methylase I (S-Adenosylmethionine: Protein-Arginine Methyltransferase; EC 2.1.1.23) From Calf Brain Original Research Article
Pages 59-67
Egon Durban, Hyang Woo Lee, Sangduk Kim, Woon Ki Paik

Chapter 6. The Methylation and Demethylation of Protein Lysine Residues Original Research Article
Pages 69-77
Samuel Nochumson, Sangduk Kim, Woon Ki Paik

Chapter 7. Purification and Assay of Protein Methylase II (S-Adenosylmethionine: Protein-Carboxyl Methyltransferase; EC 2.1.1.24) Original Research Article
Pages 79-88
Sangduk Kim, Woon Ki Paik

Chapter 8. Assessment Of Methylation In Nonhistone Chromosomal Proteins Original Research Article
Pages 89-94
C.C. Liew, D. Suria

Chapter 9. Methods for the Assessment of Histone Methylation Original Research Article
Pages 95-100
Paul Byvoet, David F. Sayre, C. Stuart Baxter

Chapter 10. Purification and Assay of Nuclear Protein Kinases Original Research Article
Pages 101-107
Valerie M. Kish, Lewis J. Kleinsmith

Chapter 11. Phosphorylation of Nonhistone Chromosomal Proteins by Nuclear Phosphokinases Covalently Linked to Agarose Original Research Article
Pages 109-117
J.A. Thomson, G.S. Stein, J.L. Steic

Chapter 12. Methods for the Assessment of Nonhistone Phosphorylation (Acid-Stable, Alkali-Labile Linkages) Original Research Article
Pages 119-126
Dorothy E. Pumo, Lewis J. Kleinsmith

Chapter 13. Methods for the Assessment of Site-Specific Histone Phosphorylation Original Research Article
Pages 127-142
Thomas A. Langan

Chapter 14. Isolation of Histone Kinases Original Research Article
Pages 143-152
Thomas A. Langan

Chapter 15. Chromosomal Protein Phosphorylation on Basic Amino Acids Original Research Article
Pages 153-159
Roberts A. Smith, Richard M. Halpern, Berndt B. Bruegger, Albert K. Dunlap, Oscar Fricke

Chapter 16. Assay of Nonhistone Phosphatase Activity Original Research Article
Pages 161-165
Lewis J. Kleinsmith

Chapter 17. Adp-Ribosylation Of Nuclear Proteins Original Research Article
Pages 167-174
Lloyd A. Stocken

Chapter 18. Chromatin-Bound Proteases and Their Inhibitors Original Research Article
Pages 175-190
Donald B. Carter, Peggy H. Efird, Chi-Bom Chae

Chapter 19. Histone Hydrolase Original Research Article
Pages 191-195
Woon Ki Paik, Sangduk Kim

Chapter 20. Translation of HeLa Cell Histone Messenger RNAs in Cell-Free Protein Synthesizing Systems from Rabbit Reticulocytes, HeLa Cells, and Wheat Germ Original Research Article
Pages 197-213
Dieter Gallwitz, Ebo Bos, Hans Stahl

Chapter 21. Isolation of Histone Messenger RNA and Its Translationin Vitro Original Research Article
Pages 215-236
Lee A. Weber, Timothy Nilsen, Corrado Baglioni

Chapter 22. In Vitro Synthesis of Single-Stranded DNA Complementary to Histone Messenger RNAs Original Research Article
Pages 237-255
C.L. Thrall, A. Lichtler, J.L. Stein, G.S. Stein

Chapter 23. Immunological Methods for the Isolation of Histone H5 mRNA from Chicken Reticulocytes Original Research Article
Pages 257-272
A.C. Scott, J.R.E. Wells

Chapter 24. Enrichment and Purification of Sea Urchin Histone Genes Original Research Article
Pages 273-286
Eric S. Weinberg, G. Christian Overton

Chapter 25. Visualization of Genes by Electron Microscopy Original Research Article
Pages 287-300
Barbara A. Hamkalo

Chapter 26. Techniques of in Vitro RNA Synthesis with Isolated Nucleoli Original Research Article
Pages 301-315
Toshio Onishi, Masami Muramatsu

Chapter 27. Transcription of RNA in Isolated Nuclei Original Research Article
Pages 317-332
William F. Marzluff Jr.

Chapter 28. Transcription of Chromatin with Heterologous and Homologous RNA Polymerases Original Research Article
Pages 333-337
Ronald H. Reeder

Chapter 29. Transcription of Chromatin with Prokaryotic and Eukaryotic Polymerases Original Research Article
Pages 339-357
Golder N. Wilson, Alan W. Steggles, W. French Anderson, Arthur W. Nienhuis

Chapter 30. Transcription of Chromatin from Cells Transformed by SV40 Virus Original Research Article
Pages 359-371
Susan M. Astrin

Chapter 31. Chromatin Reconstitution Original Research Article
Pages 373-378
R.S. Gilmour

Chapter 32. Methods for Dissociation, Fractionation, and Selective Reconstitution of Chromatin Original Research Article
Pages 379-385
G.S. Stein, J.L. Stein

Chapter 33. The Assay of Globin Gene Transcription in Reconstituted Chromatin Original Research Article
Pages 387-422
H.J. Gould, D. Maryanka, S.J. FEY, G.J. Cowling, J. Allan

Subject Index
Pages 423-426

Contents Of Previous Volumes
Pages 427-444