Chlamydia trachomatis: Methods and Protocols

Front Matter ....Pages i-xi
 Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)  (Kevin E. Eboigbodin, Mark Hoser)....Pages 1-9
 Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue  (Naraporn Somboonna, Ilada Choopara)....Pages 11-17
 Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae  (Kevin E. Eboigbodin)....Pages 19-25
 Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay  (Kevin E. Eboigbodin)....Pages 27-32
 Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth  (Tomislav Meštrović, Dezső P. Virok, Sunčanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, Jasmina Vraneš)....Pages 33-43
 Whole-Genome Sequencing of Chlamydia trachomatis Directly from Human Samples  (Amanda Claire Brown, Mette T. Christiansen)....Pages 45-67
 Multilocus Sequence Typing (MLST) of Chlamydiales  (Martina Jelocnik, Adam Polkinghorne, Yvonne Pannekoek)....Pages 69-86
 Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, A Double-Locus Genotyping Method  (Deborah C. Holt, Patiyan Andersson, Cameron Buckley, David M. Whiley, Philip M. Giffard)....Pages 87-122
 Dual RNA-Seq of Chlamydia and Host Cells  (James W. Marsh, Regan J. Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, Garry S. A. Myers)....Pages 123-135
 Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection  (Oleg V. Podgorny, Nadezhda F. Polina, Vassili N. Lazarev)....Pages 137-150
 Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions  (Katerina Wolf, Mostafa Rahnama, Kenneth A. Fields)....Pages 151-164
 Mutagenesis of Chlamydia trachomatis Using TargeTron  (Mary M. Weber, Robert Faris)....Pages 165-184
 Forward and Reverse Genetic Analysis of Chlamydia  (Mateusz Kędzior, Robert J. Bastidas)....Pages 185-204
 A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions  (Rachel Ende, Isabelle Derré)....Pages 205-218
 Rodent Infections for Chlamydia spp.  (Charles W. Armitage, Alison J. Carey, Kenneth W. Beagley)....Pages 219-236
 Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy  (Steven Liang, James B. Mahony)....Pages 237-244
 Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane  (Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, Elizabeth A. Rucks)....Pages 245-278
 High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, Including Chlamydia trachomatis  (Amanda Claire Brown, Nicole L. Kushner)....Pages 279-286
Back Matter ....Pages 287-289