Cellulases

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xix

Preface
Pages xxi-xxii
Harry J. Gilbert

Methods in Enzymology
Pages xxiii-lii

Chapter one - Measurement of endo-1,4-ОІ-Glucanase
Pages 1-17
Barry V. McCleary, Vincent McKie, Anna Draga

Chapter two - Biomass Conversion Determined via Fluorescent Cellulose Decay Assay
Pages 19-36
Bente Wischmann, Marianne Toft, Marco Malten, K.C. McFarland

Chapter three - The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method
Pages 37-50
Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

Chapter four - Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
Pages 51-67
Ondrej Kosik, Jennifer R. Bromley, Marta Busse-Wicher, Zhinong Zhang, Paul Dupree

Chapter five - Measuring Processivity
Pages 69-95
Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe, Vincent G.H. Eijsink

Chapter six - Distinguishing Xyloglucanase Activity in endo-ОІ(1В в†’В 4)glucanases
Pages 97-120
Jens M. Eklöf, Marcus C. Ruda, Harry Brumer

Chapter seven - Methods for Structural Characterization of the Products of Cellulose- and Xyloglucan-Hydrolyzing Enzymes
Pages 121-139
Maria J. PeГ±a, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O\'Neill, William S. York

Chapter eight - The Crystallization and Structural Analysis of Cellulases (and Other Glycoside Hydrolases): Strategies and Tactics
Pages 141-168
Shirley M. Roberts, Gideon J. Davies

Chapter nine - Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
Pages 169-182
Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando, Masahiro Samejima

Chapter ten - Small-Angle X-ray Scattering and Crystallography: A Winning Combination for Exploring the Multimodular Organization of Cellulolytic Macromolecular Complexes
Pages 183-210
Mirjam Czjzek, Henri-Pierre Fierobe, VГ©ronique Receveur-BrГ©chot

Chapter eleven - Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function
Pages 211-231
D. Wade Abbott, Alisdair B. Boraston

Chapter twelve - In Situ Detection of Cellulose with Carbohydrate-Binding Modules
Pages 233-245
J. Paul Knox

Chapter thirteen - Interactions Between Family 3 Carbohydrate Binding Modules (CBMs) and Cellulosomal Linker Peptides
Pages 247-259
Oren Yaniv, Felix Frolow, Maly Levy-Assraf, Raphael Lamed, Edward A. Bayer

Chapter fourteen - Approaches for Improving Thermostability Characteristics in Cellulases
Pages 261-271
Michael Anbar, Edward A. Bayer

Chapter fifteen - Thermophilic Glycosynthases for Oligosaccharides Synthesis
Pages 273-300
Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, MosГЁ Rossi, Marco Moracci

Chapter sixteen - Engineering Cellulase Activity into Clostridium acetobutylicum
Pages 301-316
Henri-Pierre Fierobe, Florence Mingardon, AngГ©lique Chanal

Chapter seventeen - Transformation of Clostridium Thermocellum by Electroporation
Pages 317-330
Daniel G. Olson, Lee R. Lynd

Chapter eighteen - Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
Pages 331-347
Jeffrey G. Gardner, David H. Keating

Chapter nineteen - Methods for the Isolation of Cellulose-Degrading Microorganisms
Pages 349-374
James E. McDonald, David J. Rooks, Alan J. McCarthy

Chapter twenty - Metagenomic Approaches to the Discovery of Cellulases
Pages 375-394
David J. Rooks, James E. McDonald, Alan J. McCarthy

Chapter twenty-one - Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes
Pages 395-415
Joana L.A. BrГЎs, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, JosГ© A.M. Prates, LuГ­s M.A. Ferreira, Maria J. RomГЈo, Harry J. Gilbert, Carlos M.G.A. Fontes

Chapter twenty-two - Measurements of Relative Binding of Cohesin and Dockerin Mutants Using an Advanced ELISA Technique for High-Affinity Interactions
Pages 417-428
Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed, Edward A. Bayer

Chapter twenty-three - Designer Cellulosomes for Enhanced Hydrolysis of Cellulosic Substrates
Pages 429-452
Yael Vazana, Sarah MoraГЇs, Yoav Barak, Raphael Lamed, Edward A. Bayer

Chapter twenty-four - High-Throughput Screening of Cohesin Mutant Libraries on Cellulose Microarrays
Pages 453-463
Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed, Edward A. Bayer

Author Index
Pages 465-487

Subject Index
Pages 489-500