Cells and Culture: Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007

Front Matter....Pages i-liv
Front Matter....Pages 1-1
Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells....Pages 3-8
RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses....Pages 9-12
Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in a 3-D Scaffold for Tissue Engineering....Pages 13-18
Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element....Pages 19-23
Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production....Pages 25-28
Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation....Pages 29-34
Cell Xpress™-Assisted Analysis of Clone Stability in Recombinant Chinese Hamster Ovary Cells....Pages 35-39
An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines....Pages 41-44
Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System....Pages 45-48
CHO-DG44 Cell Line Development by FLP-Targeting – High Level Glycoprotein Expression with Significantly Decreased Time Lines....Pages 49-55
Transgene Copy Number – Impact on Clone Performance....Pages 57-62
Single Use Bioreactors: Expressing Protein in Mammalian Cell Suspension....Pages 63-69
Front Matter....Pages 71-71
Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseases....Pages 73-78
An Avian Cell Line for Production of Highly Attenuated Viral Vectors....Pages 79-88
Microelectronic Cellular Vitality Monitoring....Pages 89-93
IFN-γ Glycosylation Macroheterogeneity, Energetic Cell Status and Medium Composition During CHO Cell Cultures....Pages 95-99
Modelling of Neural Metabolism Using 13 C-NMR Spectroscopy and Metabolic Flux Analysis....Pages 101-106
Influence of Glucose and Glutamine Concentration on Metabolism of rCHO Cells....Pages 107-111
Front Matter....Pages 113-113
Delivery of Biomolecules with Non-Viral Vectors....Pages 115-119
Circumventing the “Pay Now or Pay Later” Dilemma: Strategies for Achieving Process Development with Speed and Long-Term Potential....Pages 121-128
Front Matter....Pages 113-113
Recombinant Human Antibody Therapeutics: Supply Strategies for Early and Clinical Development from CHO Cells....Pages 129-136
Automated Screening of High Producer HEK293F Clones and Analysis of Post-Translational Modifications of Secreted Proteins....Pages 137-144
Transcriptomic and Proteomic Analysis of Antibody Producing NS0 Cells Cultivated at Different Cell Densities in Perfusion Culture....Pages 145-149
New Disposable Fixed-Bed Bioreactor for Cell Culture and Virus Production Based on a Proprietary Agitation and Aeration System....Pages 151-155
Transcriptomic Analysis of Antibody Producing NS0 Cell Line Under Hypothermic and Hypoxic Conditions....Pages 157-160
Semi-Continuous Cultures as a Tool for Cell Line Characterization During Process Development....Pages 161-164
Behaviour of GS-CHO Cell Lines in a Selection Strategy....Pages 165-169
3D Cultures: Effect on the Hepatocytes Functionality....Pages 171-176
Differential Protein Expression Induced by c-Myc Over-Expression: Proteomic Analysis of a CHO Cell Line with Increased Proliferation Capacity....Pages 177-181
Development of Pilot-Scale Orbital Shake Bioreactors: Ideal for Cost-Effective and Efficient Transient Gene Expression....Pages 183-185
Helical Tracks in Shaken Cylindrical Bioreactors Improve Oxygen Transfer and Increase Maximum Cell Density Obtainable for Suspension Cultures of Mammalian Cells....Pages 187-191
Dynamic Optimisation of CHO-IFNγ Cell Culture Fed-Batch Time-Profile....Pages 193-199
Long-Term 3D-Culture of HEP G2 Cell Line on Macroporous Ceramic Carriers....Pages 201-204
An Integrated Production Process for Human Growth Hormone....Pages 205-208
Efficient Production of Human Monoclonal Antibodies by an Improved Fructose-Based Human Cell Culture....Pages 209-212
Coupling Between Cell Kinetics and CFD to Establish Physio-Hydrodynamic Correlations in Various Stirred Culture Systems....Pages 213-217
Confocal Microscopy Observation of CHO Cells Cultivated in Presence of Fluorescent – Labelled Rapeseed Peptides....Pages 219-222
Growth and Production Characteristics of Four Mammalian Cell Lines on a Cost-Effective Serum-Free Medium....Pages 223-227
A Serum-Free, Transient Transfection System for Enhancing Production of Recombinant Antibodies in Mammalian Cells....Pages 229-232
CFD Study of the Fluid and Particle Dynamics in a Spin-Filter Perfusion Bioreactor....Pages 233-238
Front Matter....Pages 113-113
High-Yielding CHO Cell Pools for Rapid Production of Recombinant Antibodies....Pages 239-244
Increasing Upstream Process Development Efficiency by Implementing Platform Glutamine Synthetase Cell Culture Processes....Pages 245-251
Implementation of High Throughput Systems for Media and Process Development....Pages 253-255
Comparison of Cell Culture Methods for Obtaining of rHU-EPO to Large Scale....Pages 257-260
Optimization and Comparison of Different DNA Methyl Transferase and Histone Deacetylase Inhibitors for Enhancing Transient Protein Expression....Pages 261-264
Proteomic Characterisation of a Glucose-Limited CHO Perfusion Process–Analysis of Metabolic Changes and Increase in Productivity....Pages 265-269
Evaluation of Alternative Signal Sequences....Pages 271-274
Process Development for the GMP Production of N-Acetylgalactosamine-6-Sulfate Sulfatase (GALNS) Expressed by CHO Cells....Pages 275-279
Improvement of a CHO Fed-Batch Process by Fortifying with Plant Peptones....Pages 281-284
 O -Glycans on Recombinant MUC1 Produced in CHO K1 Cells Become Less Sialylated with Increased Protein Productivity, as Determined by LC-ESI MS....Pages 285-288
A Multiple Minibioreactor Platform for Parallel and Automated Mammalian Cell Culture....Pages 289-293
Cultivation of Adherent-Dependent Animal Cells on Microcarriers in a New Disposable Reactor....Pages 295-299
CHO Cells Cultivation and Antibodies Production in a New Disposable Bioreactor Based on Magnetic Driven Centrifugal Pump....Pages 301-305
Stability and Productivity of CHO Pools with Respect to Culture Age, Cryopreservation and 20 L Bioreactor Cultivation....Pages 307-311
In Vitro Disassembly and Reassembly of Triple-Layered Rotavirus-Like Particles....Pages 313-318
Influence of Culture Conditions on Insect Cell Growth and Protein Production – Comparison of Wave Bioreactor and Shake Flasks....Pages 319-324
Process Intensification Based on Nano-Structured Carbon Carrier Materials and Disposable Devices....Pages 325-330
Accelerating Fed-Batch Process Development Using Rationally Designed Feed Media....Pages 331-336
Development of a Robust Small-Scale Production Format that Is Predictive of Bioreactor Performance....Pages 337-342
Biomass Monitoring and CHO Cell Culture Optimization Using Capacitance Spectroscopy....Pages 343-348
Front Matter....Pages 113-113
Characterizing Physiology and Metabolism of High-Density CHO Cell Perfusion Cultures Using 2D-NMR Spectroscopy....Pages 349-357
BI HEX™ – Platform for Fast Track Generation of High Producer Cell Lines Leading to High-Titer Processes for Production of Therapeutic Proteins from Mammalian Cells....Pages 359-363
Management of Handling, Long-Term Stability and Shipping of Human T-Lymphocytes Bags for Clinical Studies....Pages 365-369
Mass Transfer in the CELL-tainer ® Disposable Bioreactor....Pages 371-373
Efficient Production of Human Monoclonal Antibodies by Combining In Vitro Immunization and Phage Display Methods....Pages 375-377
Measurement and Control of Viable Cell Density in a Mammalian Cell Bioprocessing Facility: Validation of the Software....Pages 379-384
Development, Validation, and Application of a Fully Integrated Automation System for Screening and Selection of High Yielding Production Cell Lines....Pages 385-389
Monitoring of Cell Activity: Online Oxygen Uptake Rates in Pulsed Aerated Cell Culture....Pages 391-393
Front Matter....Pages 395-395
Metabolite Analysis in Mammalian Cells: How to Generate Reliable Data Sets?....Pages 397-406
Cell-to-Cell Communication for Cell Density-Controlled Bioprocesses....Pages 407-412
Metabolome Analysis in Mammalian Cells: Development and Application of a Sampling Technique....Pages 413-420
Differential Expression Profiling of Industrially Relevant CHO Cell Phenotypes Using a Proprietary CHO-Specific Microarray and Proteomics Technology Platforms....Pages 421-423
Towards a Systems-Level Understanding of Increased Specific Productivity in Proliferation Arrested Myeloma NS0 Cells....Pages 425-428
Proteomic Analysis of Influenza a Virus Infected Mammalian Cells by 2D-DIGE....Pages 429-432
Involvement of SRC- and MAP Kinase-Signalings in the Effect of Sericin on Cellular Proliferation and Survival....Pages 433-435
The Unfolded Protein Response and Recombinant Protein Production from In Vitro Cultured Mammalian Cells....Pages 437-440
A Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture....Pages 441-445
Functional Analysis of ER Stress Pathway Genes for Apoptosis of NS/0 Cell Line Using RNAi Methods....Pages 447-451
Identification of New Protein Substrate Candidates of Transglutaminase in Rat Liver Extracts: Use of 5-(Biotinamido) Pentylamine as a Probe....Pages 453-458
Metabolic Flux Distributions of Adherently Growing MDCK Cells in Different Media....Pages 459-462
Front Matter....Pages 395-395
Expression of Dermal Extracellular Matrix Proteins in a Newly Developed Full-Thickness Skin Model....Pages 463-467
Development of Preparations with Antiviral and Immunostimutory Effects from Extracts of Green Parts of Coniferous Plants....Pages 469-473
Serum-Free Transfection of CHO Cells with Chemically Defined Transfection Systems to Generate Transient and Stable Cell Lines....Pages 475-478
“ Glycomics” : Development and Characterization of Glycan-Based Biotechnological Products....Pages 479-480
Development of a Cell-Culture-Based Platform for Viral Vaccine Production....Pages 481-484
Screening of Natural Compounds Affecting Type I Interferon Signalling....Pages 485-487
Front Matter....Pages 489-489
Therapeutic Proteins from Transgenic Cows’ Milk....Pages 491-496
Development of Edible Plant Vaccines....Pages 497-501
Human Cell Lines for Production of Biopharmaceuticals....Pages 503-511
Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes....Pages 513-516
Effect of ManNAc and its Derivatives on Glycosylation to Proteins Produced by Mammalian Cell Culture....Pages 517-519
Purification of a Chimeric Simian – Human Immunodeficiency Virus-Like Nanoparticle from HEK293 Cell Culture....Pages 521-527
Effects of Plant Peptones Supplemented Medium on CHO Cells....Pages 529-532
Synthetic Low Density Lipoprotein a Novel Biomimetic Lipid Supplement for Serum Free Tissue Culture....Pages 533-537
Front Matter....Pages 539-539
Scale-Down Approach for Animal-Free Polio Vaccine Production....Pages 541-550
“Novel Scaffolds for Tissue Engineering – Nano-Structured Surfaces Promote Selective Cell Attachment and Cell Differentiation”....Pages 551-557
Unconventional Experimental Concepts Enabling High Speed High Performance Media/Process Development....Pages 559-565
A Study on Bioscaffolds of Polysialic Acid and β-Glucan for Cell Culture and Tissue Engineering Applications....Pages 567-572
A Preliminary Study on Spider Silk as Biomaterial for Peripheral Nerve Regeneration....Pages 573-578
Investigation of the Effect of Mechanical Strain on the Osteogenic Differentiation of Mesenchymal Stem Cells....Pages 579-589
Front Matter....Pages 539-539
Biofunctional Polymer-Mineral Composites as Scaffolds for Bone Tissue Engineering....Pages 591-597
New Water-Soluble Polymers for Construction of Biofunctionalized Scaffolds for Bone Tissue Engineering: Synthesis and Adsorption Study....Pages 599-605
Selection of High-Producing Cells Via Cell Sorting Using an Affinity Matrix....Pages 607-611
The Effects of Medium Supplement on High-Level Production of Recombinant Human Factor IX in CHO Cell....Pages 613-618
Case Study Large Scale Cell Culture Facility....Pages 619-620
Glycosylation of Influenza A Virus Hemagglutinin....Pages 621-623
Production of Retroviral Pseudotype Vectors in Fixed Bed Reactors for Use in Gene Therapy....Pages 625-628
Swellscreen – Rapid Baculovirus Titration Method in Microplate Format....Pages 629-633
Comparing Vero and MDCK Cells for Influenza A Virus Production in Microcarrier Systems....Pages 635-638
Expansion of Human Articular Chondrocytes on Microcarriers Enhances the Production of Cartilage Specific Matrix Components....Pages 639-643
Automated Cell Culture Handling – Characteristics and Procedures....Pages 645-645
Bioreactor Satellite Culture Experiments in the Start-Up of a Cell Culture Technical Support Lab....Pages 647-655
Advantages of Hydrodynamic Cell Separation in Industrial Cell Culture Processes....Pages 657-664
Optimization and Characterization of the Process for Large Volume Cell Banking in Bags....Pages 665-668
Development of Screening Method for IgA-Promoting Factors Derived from Food Extracts Using a Human Myeloma Cell Line....Pages 669-674
Cryopreservative Solution Using Sericin....Pages 675-678
Fructan as a Novel Effective Factor for Mammalian Cell Culture....Pages 679-682
Online Determination of Oxygen Uptake and Carbon Dioxide Production Rates in Mammalian Cell Culture Using Mass Spectrometry....Pages 683-687
Optimisation of mAb Concentration in Microcarrier Based Perfusion Compared to Batch/Fed-Batch Cultivation....Pages 689-692
Characterization of the Novel Human AGE1hn Cell Line for Production of Recombinant Proteins....Pages 693-697
Front Matter....Pages 539-539
Rapid Selection of Optimal Formulations for Divergent Clones Through Screening Chinese Hamster Ovary Media Library....Pages 699-702
Development of a Vaccine Candidate Against Heartwater....Pages 703-710
Animal Component Free T-Cell Culture....Pages 711-716
Characterization of Cholesterol-Independent GS-NS/0 Recombinant Antibody Cell Lines....Pages 717-721
Development of a Fed-Batch Process for the Production of a Recombinant Protein X in CHO-GS System – Case Study from the Cell to Reactor Process Ready for Pilot Scale Cultivation....Pages 723-725
Scaffolds for Articular Joint Tissue Engineering....Pages 727-733
Effect of Tunisian Aromatic Plant Extracts on Melanogenesis....Pages 735-738
Effects of Capsaicin on Energy Metabolism by Human Intestinal Epithelial Cell Line Caco-2....Pages 739-742
Human A1AT Production – Propagating a Newly Developed Human Cell Line in a Novel Disposable Perfusion Bioreactor....Pages 743-747
Characterization of Diffusion and Flow Relations in the Novel Membrane Based Perfusion Bioreactor....Pages 749-753
Protective Effect of Di-O-Caffeoylquinic Acid on Human-Derived Neurotypic SH-SY5Y Cells Against Alzheimer’s Disease Amyloid-Beta-Induced Toxicity....Pages 755-757
Flow Through Ceramic Foams – A Future Cell Culture Challenge....Pages 759-764
Measles Virus Production in MRC-5 Cells Grown on Microcarriers in a Stirred Bioreactor....Pages 765-769
A New System for the Enrichment of Cell Subclones Secreting High Levels of IgG Using Magnetic Cell Sorting (MACS ® Technology)....Pages 771-775
Soy Hydrolysate Optimization for Cell Culture Applications....Pages 777-783
The Effect of Bioreactor pH and Temperature on Protein Glycosylation in Perfusion Cultures of Mammalian Cells....Pages 785-788
Evaluation of Cell Growth Characteristics on Chitosan-Alginate Membranes to Assess Their Potential Application on Highly Exuding Skin Lesions and In Vivo Evaluation in Wounded Cat....Pages 789-794
Vaccine Production Utilizing the Potential of Microcarriers in Disposable Bioreactor....Pages 795-801
Characterization of an Anti-Idiotypic Antibody Blocking the Capacity of the HIV-1 Specific nMAb 2F5....Pages 803-806
Cultivation of PER.C6 ® Cells in the Novel CELL-Tainer™ High-Performance Disposable Bioreactor....Pages 807-808
Front Matter....Pages 539-539
Scale-Up of a PER.C6 ® Fed-Batch Process in 50 and 250 L Hyclone Single Use Bioreactors Compared to 50 and 250 L Stainless Steel Bioreactors....Pages 809-810
Capacitance Sensor as a Robust Tool for Cell Culture Monitoring in Process Development and Manufacturing....Pages 811-817
Effect of Different Cell Culture Medium Surfactants on Cell Growth and Viability....Pages 819-822
Conventional Stirred Bioreactor Control System for Monitoring and Controlling pH and DO in a Wave Bioreactor....Pages 823-828
On-Line Monitoring of Vero Cells Cultures During the Growth and Rabies Virus Process Using Biomass Spectrometer....Pages 829-832
Inducing of Human IgE Antibodies by In Vitro Immunization....Pages 833-836
Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures....Pages 837-841
Use of Yeast Derived Nutrients for Cell Culture in Serum-Free Media....Pages 843-846
A Novel Approach to the Production of Plant-Derived Hydrolysates Yields Medium Supplements with Enhanced Performance in Cell Culture Systems....Pages 847-852
Monitoring the Cell Size Distribution of Mammalian Cell Cultures Using On-Line Capacitance Measurements....Pages 853-859
Biosimilarity of Recombinant Human EPO Products from CHO Cell Lines: A Carbohydrate Structural View....Pages 861-865
Quantitative N-Glycan Mapping of Glycoprotein Therapeutics by HPAEC-PAD: Glycosylation Characteristics of Different Recombinant Human EPO Products....Pages 867-871