Cell Culture

Content: 
Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 2
Pages ix-xii

Preface
Page xiii
P. MICHAEL CONN

1 - Multiple Approaches to Brain Culture
Pages 3-16
Cheryl F. Dreyfus, Ira B. Black

2 - Culture and Use of Primary and Clonal Neural Cells
Pages 17-33
Stephen D. Skaper, Laura Facci, Daria Milani, Alberta Leon, Gino Toffano

3 - Generation of Astrocyte Cultures from Normal and Neoplastic Central Nervous System
Pages 33-47
Sean Murphy

4 - Isolation of Astrocytes and Schwann Cells for Culture
Pages 47-56
Ramon Lim, E. Peter Bosch

5 - Culture Methods for Oligodendrocyte Cell Lines and Oligodendrocyte–Type 2 Astrocyte Lineage Cells
Pages 56-75
Jane E. Bottenstein, Samuel F. Hunter

6 - Neuropharmacological Analyses in Organotypic Cultures of Spinal Cord and Dorsal Root Ganglia
Pages 75-86
Stanley M. Crain

7 - Isolation, Culture, and Use of Viable Central Nervous System Perikarya
Pages 87-102
Gary R. Dutton

8 - Cerebral Cortical Neurons in Primary Culture and Application to Neuropharmacological Studies
Pages 103-116
Kinya Kuriyama, Seitaro Ohkuma

9 - Preparation of Highly Purified Populations of Neurons, Astrocytes, and Oligodendrocytes
Pages 119-133
Russell P. Saneto

10 - Preparation, Enrichment, and Growth of Purified Cultures of Neurons and Photoreceptors from Chick Embryos and from Normal and Mutant Mice
Pages 134-150
Ruben Adler

11 - Bulk Isolation of Mouse Oligodendrocytes by Gradient Centrifugations
Pages 153-171
Liane Bologa, Norbert Herschkowitz

12 - Bulk Isolation and Culture of Oligodendroglia from Mature Brain
Pages 171-178
William T. Norton, Muhammad Farooq

13 - Measurement of Pulsatile Hormone Release from Perifused Pituitary Cells Immobilized on Microcarriers
Pages 181-194
James R. Hansen, P. Michael Conn

14 - Cultivation of Nerve and Muscle Cells on Microcarriers
Pages 195-209
Abraham Shahar

15 - Monolayer and Three-Dimensional Culture of Rat and Human Central Nervous System: Normal and Malignant Cells and Their Interactions
Pages 210-236
Ole Didrik Laerum, Rolf Bjerkvig

16 - Brain Cell Culture in Rotation-Mediated Aggregates
Pages 236-263
Paul C. Goldsmith, Michael E. Berens

17 - Microcultures of Dissociated Primary Central Nervous System Neurons
Pages 263-275
Efrain C. Azmitia

18 - Long-Term Organotypic Culture of Central Nervous System in Maximow Assemblies
Pages 275-296
C. Dominique Toran-Allerand

19 - Preparation and Use of Morphological Variants to Investigate Neuropeptide Action
Pages 299-315
John S. Ramsdell

20 - In Vivo Tissue Culture: Use of Ectopic Anterior Pituitary Gland to Study Neuropeptide and Steroid Actions on Adenohypophysis
Pages 316-329
Frank J. Strobl, Jon E. Levine

21 - Evaluation of Catecholaminergic Activity in Hypothalamic Cultures
Pages 330-341
Barbara A. Bennett, David K. Sundberg, Mariana Morris

22 - Receptors for Bombesin/Gastrin-Releasing Peptide and Epidermal Growth Factor on Central Nervous System Cells
Pages 341-354
Terry W. Moody, Julie Staley, Mei Lee, Richard M. Kris

23 - Use of Hypothalamic Cell Cultures to Study Role of Diffusible Factors in Phenotypic Expression of Central Nervous System Neurons
Pages 355-371
Andrée Tixier-Vidal, Annie Faivre-Bauman

24 - Microinjectable Probes for Tracing Cell Lineage in Development
Pages 375-392
Duncan K. Stuart, Steven A. Torrence, Gunther S. Stent

25 - Methods for Clonal Analysis and Tracing Cell Lineages of Vertebrate Central Nervous System
Pages 393-406
Marcus Jacobson

Index
Pages 407-424