Cell and Tissue Culture Models in Dermatological Research

Front Matter....Pages I-XV
Front Matter....Pages 1-1
Development of a Cell Culture System to Detect and Analyze Pathogenicity of Autoantibodies Directed to Nuclear Antigens....Pages 3-20
Cell Cultures of Fetal Human Skin Are Suitable Models for the Investigation of Mitogenic Activity in Psoriasis Sera....Pages 21-29
Influence of Human Sera on the Adherence of Candida albicans to Buccal and Intestinal Cells in Culture....Pages 30-36
Modulation of 1,25-Dihydroxyvitamin D 3 Receptor Expression in HaCaT Keratinocytes....Pages 37-43
Aminopeptidases of Human Skin — a Survey of Enzyme Activities in Extracts from Skin and Cultured Fibroblasts....Pages 44-49
Determination of Cell Counts and Cell Proliferation by the Fluorochromes Hoechst 33342 or Hoechst 33258....Pages 50-57
Volume Determination of Adhering Cells in Culture by Means of Acoustic Interferometry....Pages 58-66
Effects of Uremic Compounds on Cultured Myocytes: a Model for Uremic Myocardiopathy....Pages 67-74
Cultivation of Trophoblast Tissue of Human Intrauterine and Ectopic Pregnancies and Treatment with Methotrexate....Pages 75-83
Front Matter....Pages 85-85
Mechanical Stimulation Enhances Human Keratinocyte Differentiation in Culture: Induction of Cytokeratin 9 Synthesis....Pages 87-97
N-Alkanes in Cultured Normal Human Keratinocytes....Pages 98-107
Influence of Retinoids on the Keratin Profile in Normal and Spontaneously Transformed Human Keratinocytes....Pages 108-117
Cytological Characterization of Three-dimensional, Graftable Human Keratinocyte Cultures....Pages 118-125
Fluorometric Measurement of Suprabasal Cytokeratins and DNA of Human Keratinocytes Cultivated in Microtiter Plates....Pages 126-131
Front Matter....Pages 133-133
Biochemistry of Mammalian Pigmentation: Enzymatic Regulation of Melanogenesis....Pages 135-147
Cultured Epidermal Melanocytes from Patients with Neurofibromatosis 1 (Recklinghausen’s Disease): a Cell Culture Model for Neurofibromatosis 1....Pages 148-154
Pigmentation in Cultures of Human Melanocytes....Pages 155-163
Front Matter....Pages 165-165
Re-epithelialization of Incision and Burn Wounds in Culture....Pages 167-175
Primary Culture of Bilayered Epidermal Cell Sheets....Pages 176-182
Plasminogen Activator Contained in Secretions of a Keratinocyte Cell Line and in the Fluid of Intraepidermal Pemphigus vulgaris Blisters Is Activated by Sodium dodecyl sulfate....Pages 183-188
Front Matter....Pages 165-165
Are Rat Granulation Tissue Fibroblasts Regulated by Cytokines in the Same Manner as Skin Fibroblasts?....Pages 189-199
Cultured Allografts for Wound Conditioning and Enhanced Epithelialization of Chronic Venous Ulcers....Pages 200-207
Collagen Type-I Is Chemoattractive for the Human Keratinocyte Cell Line (HaCaT)....Pages 208-218
Front Matter....Pages 219-219
The Molecular Biology of the Terminal Differentiation of Dermal Fibroblasts in the Fibroblast Stem Cell System in Vivo and In Vitro....Pages 221-229
Cytokine-dependent Regulation of Human Dermal Fibroblasts Cultured in a Three-dimensional Collagen-Gel....Pages 230-236
Investigation of Components of the Dermal-Epidermal Junction Zone In Vitro: Effect of Transforming Growth Factor-beta on Collagen VII Expression....Pages 237-240
The Influence of Retinoids on Chemotaxis and Connective Tissue Synthesis of Fibroblasts....Pages 241-248
Front Matter....Pages 249-249
In Vitro Studies of Fibroblast Aging: Growth Factors, Collagen and Glycosaminoglycan Synthesis — a Review....Pages 251-263
Terminal Differentiation, Aging, Apoptosis, or Transformation of the WI-38 Fibroblasts in the Fibroblast Stem Cell System In Vitro....Pages 264-271
Differential Gene Expression, Protein Synthesis, and Degradation in Aging Fibroblasts....Pages 272-277
Age Differences in Human Skin Collagen....Pages 278-282
Influence of Cell Density on Energy Metabolism and the Actin Skeleton of Different Cell Types....Pages 283-291
Front Matter....Pages 293-293
(+)-Aeroplysinin-l: an Inhibitor of Intrinsic Protein Tyrosine Kinase Activity of EGF-Receptor-Kinase Complex....Pages 295-301
In Vitro Cultivation of Human Dermal Microvascular Endothelial Cells as a Model for the Dermatopharmacology....Pages 302-314
Effect of Nicotine on the Differentiation of Human Keratinocytes In Vitro....Pages 315-323
A New In Vitro Test System and Phototoxicological Studies on Cell Cultures Based on the Melamine Substrate Foil....Pages 324-333
In Vitro Assays for Evaluation of the Effects of 8-Methoxypsoralen and UV Irradiation on Human Lymphocytes and Granulocytes....Pages 334-340
Comparative Cytotoxicity Test with Human Keratinocytes, HaCaT Cells, and Skin Fibroblasts to Investigate Skin-Irritating Substances....Pages 341-347
SDS-Induced Activation of the Cutaneous Plasminogen Activator System: A Molecular Pathway Possibly Involved in SDS-Induced Skin Irritation....Pages 348-354
Back Matter....Pages 355-364