Cancer Metabolism: Methods and Protocols

Front Matter ....Pages i-xi
 Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction  (Daniel R. Crooks, Teresa W.-M. Fan, W. Marston Linehan)....Pages 1-27
 Imaging Cancer Metabolism with Positron Emission Tomography (PET)  (Timothy H. Witney, David Y. Lewis)....Pages 29-44
 Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells  (Debanti Sengupta, Guillem Pratx)....Pages 45-53
 Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation Dynamics in Cancer Cell Metabolism and Epigenetics  (Alice C. Newman, Christiaan F. Labuschagne, Karen H. Vousden, Oliver D. K. Maddocks)....Pages 55-67
 Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)  (Sarah Creed, Matthew McKenzie)....Pages 69-76
 Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells  (David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, Carmen Chak-Lui Wong)....Pages 77-99
 Determination of Polarization of Resident Macrophages and Their Effect on the Tumor Microenvironment  (Ioannis S. Pateras, Tomer Cooks)....Pages 101-112
 Quantitation of Macropinocytosis in Cancer Cells  (Koen M. O. Galenkamp, Basheer Alas, Cosimo Commisso)....Pages 113-123
 Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation  (Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, Alessandro Carrer)....Pages 125-147
 Methods to Measure Autophagy in Cancer Metabolism  (Natalia von Muhlinen)....Pages 149-173
 Lipidomic Analysis of Cancer Cell and Tumor Tissues  (Islam Sk Ramiz, Soumen Kanti Manna)....Pages 175-204
 Mass Spectrometry-Based Profiling of Metabolites in Human Biofluids  (Tanushree Chakraborty, Soumen Kanti Manna)....Pages 205-234
 Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile Through Extraction and Derivatization of Polar Metabolites  (Jodi Rattner, Farshad Farshidfar, Oliver F. Bathe)....Pages 235-249
 Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy  (Beata Mickiewicz, M. Eric Hyndman, Hans J. Vogel)....Pages 251-274
 Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging  (Shibdas Banerjee, Soumen Kanti Manna)....Pages 275-297
 Compositional Analysis of the Human Microbiome in Cancer Research  (Elisa Morales, Jun Chen, K. Leigh Greathouse)....Pages 299-335
 Assessing Metabolic Dysregulation in Muscle During Cachexia  (Myriam Y. Hsu, Paolo E. Porporato, Elisabeth Wyart)....Pages 337-352
 Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells  (Jing Zhang, Qing Zhang)....Pages 353-363
 Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging  (Thomas S. Blacker, Michael D. E. Sewell, Gyorgy Szabadkai, Michael R. Duchen)....Pages 365-387
 Overview of Characterizing Cancer Glycans with Lectin-Based Analytical Methods  (Amanda J. Pearson, Elyssia S. Gallagher)....Pages 389-408
 Hyperpolarized MRI for Studying Tumor Metabolism  (Mikko I. Kettunen)....Pages 409-426
 Overview of Glutamine Dependency and Metabolic Rescue Protocols  (Shuo Qie, Dan He, Nianli Sang)....Pages 427-439
 Integration of Metabolomics and Transcriptomics to Identify Gene-Metabolite Relationships Specific to Phenotype  (Andrew Patt, Jalal Siddiqui, Bofei Zhang, Ewy Mathé)....Pages 441-468
 Biclustering Analysis of Co-regulation Patterns in Nuclear-Encoded Mitochondrial Genes and Metabolic Pathways  (Robert B. Bentham, Kevin Bryson, Gyorgy Szabadkai)....Pages 469-478
 Using the Human Genome-Scale Metabolic Model Recon 2 for Steady-State Flux Analysis of Cancer Cell Metabolism  (Lake-Ee Quek, Nigel Turner)....Pages 479-489
Back Matter ....Pages 491-495