Borrelia burgdorferi: Methods and Protocols

Front Matter ....Pages i-xiv
 Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue  (Alan G. Barbour, Vanessa J. Cook)....Pages 1-11
 Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods  (Gabriele Margos, Isabell Notter, Volker Fingerle)....Pages 13-33
 Generation of Mammalian Host-Adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats  (Melissa J. Caimano)....Pages 35-45
 Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi  (Henna Iqbal, Darrin R. Akins, Melisha R. Kenedy)....Pages 47-55
 Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix  (Yi-Pin Lin, John M. Leong)....Pages 57-67
 Analysis of Lipids and Lipid Rafts in Borrelia  (Alvaro Toledo, Zhen Huang, Jorge L. Benach, Erwin London)....Pages 69-82
 Trace Element Analysis of Borrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry  (Xin Li, Peng Wang, Anthony Lutton, John Olesik)....Pages 83-94
 Identification and Characterization of Borrelia burgdorferi Complement-Binding Proteins  (Peter Kraiczy)....Pages 95-103
 Artificial Infection of Ticks with Borrelia burgdorferi Using a Microinjection Method and Their Detection In Vivo Using Quantitative PCR Targeting flaB RNA  (Alexis A. Smith, Xiuli Yang, Erol Fikrig, Utpal Pal)....Pages 105-114
 Borrelia burgdorferi Microarray Analysis  (Radha Iyer, Ira Schwartz)....Pages 115-126
 Borrelia burgdorferi Transcriptome Analysis by RNA-Sequencing  (M Lybecker, KC Henderson)....Pages 127-136
 Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology  (Philip P. Adams, Mollie W. Jewett)....Pages 137-154
 Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins  (J. Seshu, Trever C. Smith II, Ying-Han Lin, S. L. Rajasekhar Karna, Christine L. Miller, Tricia Van Laar)....Pages 155-175
 Identification of Acetylated Proteins in Borrelia burgdorferi  (Youyun Yang, Alan Wolfe, X. Frank Yang)....Pages 177-182
 Genetic Transformation and Complementation  (D. Scott Samuels, Dan Drecktrah, Laura S. Hall)....Pages 183-200
 Genome-Wide Mutagenesis in Borrelia burgdorferi  (Tao Lin, Lihui Gao)....Pages 201-223
 Generation of Conditional Mutants in Borrelia burgdorferi  (Jacob I. Latham, Jon S. Blevins)....Pages 225-239
 Detection of Bioluminescent Borrelia burgdorferi from In Vitro Cultivation and During Murine Infection  (Jenny A. Hyde, Jon T. Skare)....Pages 241-257
 Analysis of Borrelia burgdorferi Proteome and Protein–Protein Interactions  (Xiuli Yang, Meghna Thakur, Juraj Koci, Alexis A. Smith, Preeti Singh, Xuran Zhuang et al.)....Pages 259-277
 Two Photon Intravital Microscopy of Lyme Borrelia in Mice  (Alexia A. Belperron, Jialing Mao, Linda K. Bockenstedt)....Pages 279-290
 Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi  (Lauren M. K. Mason, Joppe W. R. Hovius)....Pages 291-299
 Phagocytosis Assays for Borrelia burgdorferi  (Juan Anguita, Ana Carreras-González, Nicolás Navasa)....Pages 301-312
 Measuring Borrelia burgdorferi Motility and Chemotaxis  (Kai Zhang, Chunhao Li)....Pages 313-317
 In Vitro Models of Cutaneous Inflammation  (Quentin Bernard, Benoît Jaulhac, Nathalie Boulanger)....Pages 319-327
 Magnetic Isolation of Phagosomes Containing Toll-Like Receptor Ligands  (Tanja Petnicki-Ocwieja, Linden T. Hu)....Pages 329-336
 Xenodiagnosis Using Ixodes scapularis Larval Ticks in Humans  (Siu-Ping Turk, Carla Williams, Adriana Marques)....Pages 337-346
 Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization  (Maria Gomes-Solecki, Luciana Richer)....Pages 347-357
Back Matter ....Pages 359-361