Bone Research Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Isolation and Generation of Osteoclasts  (Nadia Rucci, Alberta Zallone, Anna Teti)....Pages 3-19
 Isolation and Generation of Osteoblasts  (Inês P. Perpétuo, Lucie E. Bourne, Isabel R. Orriss)....Pages 21-38
 Isolation, Purification, Generation, and Culture of Osteocytes  (Jonathan H. Gooi, Ling Yeong Chia, Christina Vrahnas, Natalie A. Sims)....Pages 39-51
 Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo  (Janos Kanczler, Rahul S. Tare, Patrick Stumpf, Timothy J. Noble, Cameron Black, Richard O. C. Oreffo)....Pages 53-70
 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies  (Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan)....Pages 71-98
 Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation  (Soraia P. Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon C. F. Rawlinson, Andrew A. Pitsillides)....Pages 99-128
Front Matter ....Pages 129-129
 Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation  (Silvia Marino, Aymen I. Idris)....Pages 131-143
 Analysis of Transcriptional Regulation in Bone Cells  (Huilin Jin, Antonia Sophocleous, Asim Azfer, Stuart H. Ralston)....Pages 145-167
 Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization  (Brice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, Francois Lamoureux)....Pages 169-196
Front Matter ....Pages 197-197
 The Ex Vivo Organ Culture of Bone  (Katherine A. Staines, Genevieve Brown, Colin Farquharson)....Pages 199-215
 Ex-Vivo Model Systems of Cancer-Bone Cell Interactions  (Erdjan Salih)....Pages 217-240
 Mechanical Properties of Bone Ex Vivo  (Simon R. Goodyear, Richard M. Aspden)....Pages 241-257
Front Matter ....Pages 259-259
 Ovariectomy/Orchiectomy in Rodents  (Antonia Sophocleous, Aymen I. Idris)....Pages 261-267
 In Vivo Models of Rheumatoid Arthritis  (Bettina Grötsch, Aline Bozec, Georg Schett)....Pages 269-280
 Osteoarthritis Mouse Model of Destabilization of the Medial Meniscus  (Antonia Sophocleous, Carmen Huesa)....Pages 281-293
 Models of Prostate Cancer Bone Metastasis  (Sun Hee Park, Matthew Robert Eber, Yusuke Shiozawa)....Pages 295-308
 Animal Models of Breast Cancer Bone Metastasis  (Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, Penelope Ottewell)....Pages 309-330
 Murine Models of Bone Sarcomas  (Camille Jacques, Nathalie Renema, Benjamin Ory, Carl R. Walkley, Agamemnon E. Grigoriadis, Dominique Heymann)....Pages 331-342
 Mouse Models of Melanoma Bone Metastasis  (Özge Uluçkan)....Pages 343-348
 Animal Models of Multiple Myeloma  (Sam W. Z. Olechnowicz, Claire M. Edwards)....Pages 349-360
 The Calvarial Injection Assay  (Robert J. van ’t Hof)....Pages 361-368
 In Vivo Models of Mechanical Loading  (Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon et al.)....Pages 369-390
 Animal Models for the Study of Bone-Derived Pain  (Austen L. Thompson, Tally M. Largent-Milnes, Todd W. Vanderah)....Pages 391-407
Front Matter ....Pages 409-409
 Histomorphometry in Rodents  (Reinhold G. Erben, Martin Glösmann)....Pages 411-435
 Immunostaining of Skeletal Tissues  (Anke J. Roelofs, Cosimo De Bari)....Pages 437-450
 Techniques for the Study of Apoptosis in Bone  (Val Mann, Brendon Noble)....Pages 451-465
 Live Cell Imaging of Bone Cell and Organ Cultures  (Sarah L. Dallas, Patricia A. Veno, LeAnn M. Tiede-Lewis)....Pages 467-506
 Analysis of Bone Architecture in Rodents Using Micro-Computed Tomography  (Robert J. van ’t Hof, Enrico Dall’Ara)....Pages 507-531
 Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents  (Jürg Andreas Gasser, Johannes Willnecker)....Pages 533-558
 Quantitative X-Ray Imaging of Mouse Bone by Faxitron  (Natalie C. Butterfield, John G. Logan, Julian Waung, Graham R. Williams, J. H. Duncan Bassett)....Pages 559-569
 Scanning Electron Microscopy of Bone  (Alan Boyde)....Pages 571-616
 Transmission Electron Microscopy of Bone  (Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, Wouter Beertsen)....Pages 617-629
 Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy  (Gloria Allocca, Ning Wang)....Pages 631-639
 Fourier Transform Infrared Imaging of Bone  (Eleftherios P. Paschalis)....Pages 641-649
 Raman Microscopy and Bone  (Simon R. Goodyear, Richard M. Aspden)....Pages 651-659
Back Matter ....Pages 661-670