Biological Field Emission Scanning Electron Microscopy

Content: Intro
 Table of Contents
 About the Editors
 List of Contributors
 Foreword
 Volume I
 1 Scanning Electron Microscopy: Theory, History and Development of the Field Emission Scanning Electron Microscope
 1.1 The Scanning Electron Microscope
 1.2 The Thermionic GUN
 1.3 The Lanthanum Hexaboride ("LaB6") Source
 1.4 Other Enhanced "higher Brightness" Sources
 1.5 The Twenty‐first Century SEM
 1.6 The Future for Ion Beam Imaging --
Above and Beyond
 References
 2 Akashi Seisakusho Ltd --
SEM Development 1972-1986
 2.1 Introduction
 2.2 TEM Development
 2.3 SEM Development, with TEM Repercussions 2.4 TEM Again, but SEM Lives on2.5 MSM (Mini Scanning Microscope) Table‐top Series
 2.6 SIGMA (SS) Series
 Acknowledgements
 References
 3 Development of FE‐SEM Technologies for Life Science Fields
 3.1 Introduction
 3.2 Principle of SEM and Mechanism of Resolution
 3.3 Commercialization of Fe‐sem and the Impact of Its Application
 3.4 Development of In‐lens Fe‐sem And Its Impact
 3.5 Introduction of SEMI In‐lens Fe‐sem
 3.6 Resolution Improvement by the Deceleration Method
 3.7 Popularization of a Schottky Emission Electron Source and Progress of a Cold FE Electric Gun 3.8 Advent of Truly "Easy to Use" FE‐SEM3.9 Closing Remarks
 Acknowledgements
 References
 4 A History of JEOL Field Emission Scanning Electron Microscopes with Reference to Biological Applications
 4.1 The First Jeol Scanning Electron Microscopes
 4.2 The First Cryo‐sem
 4.3 Development of Jeol Field Emission Sems
 4.4 In‐lens Field Emission SEM Development
 4.5 Introduction of the JEOL SEMI in‐Lens
 4.6 Evolution of the JEOL Semi In‐lens FE‐SEM
 4.7 Development of Beam Deceleration and the JEOL Energy Filter
 4.8 A Unique Aberration Corrected Fe‐sem 4.9 Ongoing Semi In‐lens Fe‐sem Development4.10 JEOL Introduces the Super Hybrid Lens
 4.11 Development of the JEOL Gentle Beam System
 4.12 Conclusion
 References
 5 TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy
 5.1 Historical Introduction
 5.2 Biological Samples in SEM
 5.3 Methods For Imaging Biological Samples
 5.4 Imaging of Sensitive Samples
 5.5 Advanced Fe‐sem Techniques in Biology
 Conclusions
 Acknowledgements
 References
 6 FEG‐SEM for Large Volume 3D Structural Analysis in Life Sciences
 6.1 Introduction 6.2 High Resolution SEM Imaging at Low Accelerating Voltage6.3 Spot Size
 6.4 Beam Penetration
 6.5 Contrast and Signal‐to‐noise Ratio
 6.6 Serial Block Face Imaging
 6.7 Challenges of SBFI
 6.8 Recent Advances
 6.9 Multienergy Deconvolution
 6.10 Stability
 6.11 Speed
 6.12 Automated Acquisition, Reconstruction, and Analysis
 6.13 Conclusion
 References
 7 ZEISS Scanning Electron Microscopes for Biological Applications
 7.1 Biological Imaging using Zeiss Technology
 7.2 3D Imaging --
Live Happens in 3D
 7.3 Correlative Solutions --
for a Deeper Insight
 Acknowledgement
 References