Biological applications of magnetic resonance

Front Cover......Page 1
Title Page
......Page 4
Copyright Page......Page 5
Table of contents......Page 6
CONTRIBUTORS......Page 8
I. INTRODUCTION......Page 10
II. LOCATION OF HYDROGEN-BONDED PROTON RESONANCES OF NUCLEIC ACIDS......Page 11
III. RING CURRENT SHIFT CALCULATIONS......Page 17
IV. EXCHANGE OF HYDROGEN-BONDED PROTONS......Page 20
V. EXCHANGE OF OLIGONUCLEOTIDES......Page 23
VI. EXCHANGE IN TRANSFER RNAS......Page 35
REFERENCES......Page 49
I. INTRODUCTION......Page 54
II. HYDROGEN-BONDED PROTONS......Page 56
III. ASSIGNMENTS VIA CALCULATIONS......Page 87
IV. LOW-FIELD RESONANCE ASSIGNMENT VIA NUCLEOTIDE FRAGMENTS......Page 108
V. THE -11 ppm TO -9 ppm SPECTRAL REGION......Page 111
VI. HAVE WE LEARNED ANYTHING WE DIDN' 'T ALREADY KNOW?......Page 114
ACKNOWLEDGMENTS......Page 116
REFERENCES......Page 117
I. INTRODUCTION......Page 122
III. CHEMICAL SHIFTS......Page 125
IV. EFFECTS OF THE SELF-AGGREGATION OF DRUGS ON THE CHEMICAL SHIFTS......Page 130
V. CHEMICAL SHIFTS IN CARBON-13 SPECTRA......Page 132
VI. CHEMICAL SHIFTS IN 31p SPECTRA OF DRUG-NUCLEIC ACID COMPLEXES......Page 135
VIII. NUCLEAR RELAXATION EXPERIMENTS......Page 142
IX. KINETIC PARAMETERS......Page 145
X. A BRIEF HISTORICAL INTRODUCTION TO THE NMR STUDIES OF DRUG-NUCLEIC ACID COMPLEXES......Page 146
XI. 9-AMINOACRIDINE......Page 150
XII. MUTAGEN-OLIGONUCLEOTIDE COMPLEXES WITH A BULGED BASE......Page 153
XIII. BIPHASIC HELIX-COIL TRANSITIONS OF INTERCALATING DRUG-NUCLEIC ACID COMPLEXES......Page 154
XIV. ACTINOMYCIN D-DEOXYOLIGONUCLEOTIDE COMPLEXES......Page 156
XV. ETHIDIUM BROMIDE-OLIGONUCLEOTIDE COMPLEXES......Page 164
XVII. SEQUENCE PREFERENCES......Page 170
XVIII. GEOMETRY OF THE HELIX AT THE INTERCALATION SITE......Page 173
XIX. SUGAR PUCKER IN SOLUTION STUDIES......Page 174
XX. CONCLUDING REMARKS......Page 177
REFERENCES......Page 178
I. INTRODUCTION: DEFINITION OF THE NUCLEAR OVERHAUSER EFFECT......Page 186
II. LOW-MOLECULAR-WEIGHT COMPOUNDS—THE EXTREME NARROWING LIMIT......Page 191
III. BIOPOLYMERS AND INTERMEDIATE TO LONG CORRELATION TIMES......Page 208
REFERENCES......Page 225
ACKNOWLEDGMENT......Page 228
CHAPTER 5. PULSED EPR STUDIES OF METALLOPROTEINS......Page 230
I. THE ELECTRON SPIN ECHO MECHANISM......Page 231
II. THE LINEAR ELECTRIC FIELD EFFECT IN EPR......Page 238
III. ELECTRON NUCLEAR COUPLING IN SPIN ECHO EXPERIMENTS......Page 258
IV. THE DESIGN OF ELECTRON SPIN ECHO EXPERIMENTS......Page 275
REFERENCES......Page 276
ACKNOWLEDGMENTS......Page 278
I. INTRODUCTION......Page 280
II. MODEL BUILDING AND MAGNETIC RESONANCE STUDIES: AN APPROACH TO ANTIBODY SPECIFICITY......Page 283
III. GENERAL FEATURES OF Fv FRAGMENT-HAPTEN INTERACTIONS......Page 285
IV. DIMENSIONS AND POLARITY OF THE COMBINING SITE......Page 288
V. THE ENVIRONMENT OF THE HAPTEN AROMATIC RING......Page 290
VI. THE BINDING OF Tnp HAPTENS TO THE Fv FRAGMENT: A STUDY OF CROSS-REACTION BY NMR......Page 305
VII. SUMMARY AND CONCLUSIONS......Page 307
ACKNOWLEDGMENTS......Page 310
REFERENCES......Page 311
I. INTRODUCTION......Page 314
II. PRINCIPLES......Page 318
III. MODEL COMPOUNDS......Page 319
IV. LOCATION AND ASSIGNMENT OF RESONANCES......Page 322
V. HYPERFINE SHIFT ORIGINS......Page 336
VI. HYPERFINE SHIFT CHANGES WITH MOLECULAR/ELECTRONIC STRUCTURE......Page 337
REFERENCES......Page 348
I. INTRODUCTION......Page 354
II. 31P NMR OF PHOSPHORUS-CONTAINING INTERMEDIATES OF THE ALKALINE PHOSPHATASE REACTION......Page 358
III. LIGANDS TO THE CATALYTIC METAL IONS AS DETERMINED BY 13C NMR......Page 378
IV. 113Cd NUCLEAR MAGNETIC RESONANCE OF Cd(II) ALKALINE PHOSPHATASE......Page 384
V. 19F NMR OF FLUORINATED AMINO ACIDS INCORPORATED INTO ALKALINE PHOSPHATASE......Page 390
VI. SUMMARY AND CONCLUSIONS......Page 394
REFERENCES......Page 395
APPENDIX: EFFECTS OF CHEMICAL EXCHANGE ON 113Cd RESONANCES......Page 399
I. INTRODUCTION......Page 406
II. NUCLEAR MAGNETIC RESONANCE STUDIES......Page 413
III. CONCLUSIONS AND FUTURE NEEDS......Page 458
ACKNOWLEDGMENTS......Page 465
REFERENCES......Page 466
I. INTRODUCTION......Page 472
II. INTERPRETATION OF TISSUE SPECTRA......Page 479
III. THE ATTAINMENT OF STABLE PHYSIOLOGICAL CONDITIONS IN THE SPECTROMETER......Page 495
IV. BIOCHEMICAL AND PHYSIOLOGICAL RESULTS......Page 501
V. THE USE OF OTHER NUCLEI......Page 531
VI. POSTSCRIPT......Page 534
REFERENCES......Page 535
APPENDIX 1......Page 539
I. INTRODUCTION......Page 546
II. METHODS......Page 547
III. 31P NMR SPECTRA  AND ASSIGNMENTS......Page 548
IV. BIOENERGETICS......Page 553
IV. RATE MEASUREMENTS IN VIVO......Page 574
V. 13C NMR STUDIES......Page 584
VI. CONCLUSION......Page 594
REFERENCES......Page 595
SUBJECT INDEX......Page 600