Biochemistry of Vitamin B6 and PQQ

Front Matter....Pages I-XVII
History of Vitamin B6....Pages 1-5
Front Matter....Pages 7-7
Molecular evolution of pyridoxal-5′-phosphate-dependent enzymes....Pages 9-13
Stereospecificity of aminotransferases for C-4′ hydrogen transfer and enzyme evolution....Pages 15-19
Detection of weak structural similarities among PLP dependent enzymes....Pages 21-25
Front Matter....Pages 27-27
Crystallographic studies on the vitamin B 6 -assisted enzymic transamination reaction....Pages 29-35
Redesign of aspartate aminotransferase specificity to that of tyrosine aminotransferase....Pages 37-41
Enzyme-substrate interactions modulating protonation and tautomerization states of the aldimines of pyridoxal enzymes....Pages 43-47
Functional role of the mobile stretch Gly36-Val37-Gly38 of porcine cytosolic aspartate aminotransferase....Pages 49-53
Using 1 H-and 15 N-NMR spectroscopy to observe active sites of porcine cytosolic and Escherichia coli aspartate aminotransferases....Pages 55-59
Aspartate aminotransferases like it hot....Pages 61-65
Studies on the mechanism of action of D-amino acid transaminase....Pages 67-73
Cytosolic factors and the twisting path from birth to berth in aspartate aminotransferases....Pages 75-80
Mitochondrial protein import: role of certain domains of mitochondrial aspartate aminotransferase....Pages 81-85
Cytosolic aspartate aminotransferase : tissue-specific hormonal regulation of a housekeeping gene promoter....Pages 87-92
Regulation by glucagon of serine:pyruvate/alanine:glyoxylate aminotransferase gene expression in cultured hepatocytes....Pages 93-97
Glutamate 1-semialdehyde aminotransferase, a unique enzyme in chlorophyll biosynthesis....Pages 99-103
Mechanism of glutamate semialdehyde aminotransferase probed with substrate analogues....Pages 105-109
Front Matter....Pages 111-111
Crystallographic and kinetic studies of the tryptophan synthase α 2 β 2 complex with a mutation in β subunit lysine-87 that binds pyridoxal phosphate....Pages 113-117
The Roles of Chemical Transformation, Loop Closure, Tunnel Function and Metal Ion Activation in the Tryptophan Synthase Mechanism....Pages 119-124
Effects of Monovalent Cations on Functional Properties of the Tryptophan Synthase α 2 β 2 Complex in Solution and in the Crystal....Pages 125-129
Front Matter....Pages 111-111
The long-range effect on the mutual activation of the α and β subunits in the α 2 β 2 complex of tryptophan synthase....Pages 131-133
X-ray Structure, Sequence and Solution Properties of Ornithine Decarboxylase from Lactobacillus 30a ....Pages 135-139
Dialkylglycine decarboxylase structure: alkali metal binding sites and bifunctional active site....Pages 141-145
The Location and Role of Active-Site Bases in PLP-Dependent Decarboxylase Enzymes as Deduced from Stereochemical and Kinetic Studies....Pages 147-151
GuCI-induced unfolding of pig kidney dopa decarboxylase: Evidence for a multi-state process....Pages 153-157
Front Matter....Pages 159-159
The transition state complex in the phosphorylase catalyzed reaction....Pages 161-166
Binding mode of glucans to α-glucan phosphorylase isozymes and their chimeric enzymes....Pages 167-171
Slow conformational transitions of muscle glycogen phosphorylase b ....Pages 173-180
Front Matter....Pages 181-181
X-ray study of tryptophanase at 2.1 Å resolution....Pages 183-185
Crystallographic studies of tyrosine phenol-lyase....Pages 187-191
Studies of the Mechanism of Tyrosine Phenol-lyase: Kinetics and Site-Directed Mutagenesis....Pages 193-197
The role of pyridoxal phosphate in the refolding of serine hydroxymethyltransferase....Pages 199-203
The Mechanism of O-Acetylserine Sulfhydrylase from Salmonella typhimurium ....Pages 205-209
Involvement of PLP in biological formation iron-sulfur clusters....Pages 211-215
Iron-sulfur clusters as alternatives to pyridoxal-5′-phosphate in bacterial L-serine dehydratases: Cloning of the gene encoding the β-subunit of the enzyme from Peptostreptococcus asaccharolyticus ....Pages 217-221
Phosphorescence of Pyridoxal Kinase at Room Temperature: The Effects of Ligands Bound to the Nucleotide Site....Pages 223-228
The Schiff base of pyridoxal-5′-phospate with ethylenediamine and ethylamine: Species in solution....Pages 229-231
Front Matter....Pages 233-233
Biological role and reactivity of TPQ containing amine oxidases....Pages 235-240
Two distinct quinoprotein amine oxidases are functioning in Aspergillus niger ....Pages 241-245
The catalytic competence of cobalt-amine oxidase....Pages 247-251
Front Matter....Pages 233-233
Mechanistic Studies of Copper/Topa Amine Oxidases....Pages 253-257
Design, synthesis and evaluation of mechanism-based inhibitors of copper amine oxidases....Pages 259-261
Copper ion-dependent biogenesis of topa quinone cofactor covalently bound to bacterial monoamine oxidase....Pages 263-267
Modulation of lysyl oxidase activity toward peptidyl lysine by vicinal dicarboxylic amino acid residues....Pages 269-273
Front Matter....Pages 275-275
The structure and function of quinoprotein dehydrogenases....Pages 277-281
Quinoprotein oxidoreductases for the oxidation of alcohols, sugars and amines....Pages 283-289
The ternary complex between methylamine dehydrogenase, amicyanin and cytochrome c 551i ....Pages 291-295
Model studies directed toward the action of quinoprotein methanol dehydrogenase....Pages 297-301
Front Matter....Pages 303-303
Nutritional aspects of vitamin B 6 : requirements, recommendations and intake....Pages 305-309
Vitamin B 6 transport and metabolism: Clues for delivery of bioactive compounds....Pages 311-317
Vitamin B 6 Modulation of Steroid Receptor-mediated Gene Expression....Pages 319-327
Front Matter....Pages 329-329
Pyridoxine (vitamin B 6 ), neurotransmitters and hypertension....Pages 331-335
A liver enzyme, serine:pyruvate/alanine:glyoxylate aminotransferase and its mutant in a primary hyperoxaluria type 1 case....Pages 337-341
Pyridoxal Phosphate, GABA and Seizure Susceptibility....Pages 343-347
Monoclonal antibodies to bovine brain GABA transaminase....Pages 349-351
Effect of pyrroloquinoline quinone (PQQ) on the SLE-like (Systemic Lupus Erythematosus) disease in MRL-Ipr/Ipr mice....Pages 353-357
Conclusions....Pages 359-360
Back Matter....Pages 361-366