Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins

Front Matter....Pages I-XV
Front Matter....Pages 1-1
Genetic and Genomic Approaches for Delineating the Pathway of Pyridoxal 5’-Phosphate Coenzyme Biosynthesis in Escherichia coli ....Pages 3-10
Enzymes Catalysing Formation of Pyridoxal Phosphate From Vitamin B 6 ....Pages 11-15
A Divergence in the Biosynthetic Pathway and a New Role for Vitamin B6....Pages 17-22
Molecular Cloning and Catalytic Properties of Human Brain Pyridoxal Kinase....Pages 23-26
Front Matter....Pages 27-27
Regulation of the aspartate and alanine aminotransferases in humans and rodents....Pages 29-34
Mimosine’s Mechanism is Pyridoxal-Phosphate Independent....Pages 35-40
Environmental Stimuli and Regulatory Factors Affecting the Expression of the Glutamic Acid Decarboxylase System in Escherichia coli ....Pages 41-46
Front Matter....Pages 47-47
The membrane glucose dehydrogenase of Escherichia coli ....Pages 49-54
Structural properties of homodimeric quinoprotein ethanol deyhdrogenase from....Pages 55-60
Physiological Importance of Pyrroloquinoline Quinone....Pages 61-66
Mechanism of Topa Quinone Biogenesis in Copper Amine Oxidase Studied by Site-Directed Mutagenesis and X-Ray Crystallography....Pages 67-70
Lysyl Oxidase Activates the Transcription Activity of Collagen III Promoter: Possible Involvement of Ku Antigen....Pages 71-76
TGF-ß1 Regulation of Gingival Lysyl Oxidase and Connective Tissue Growth Factor....Pages 77-82
Pyridoxine, dopa decarboxylase, and tetrahydroisoquinoline derivatives in Parkinson’s disease....Pages 83-88
Front Matter....Pages 89-89
Lysyl oxidase: a family of multifunctional proteins....Pages 91-96
Structural Aspects of Lysyl Oxidase....Pages 97-100
Chemotaxis of Vascular Smooth Muscle Cells by Lysyl Oxidase....Pages 101-107
Front Matter....Pages 109-109
Biological Implications of the Different Hsp70 Binding Properties of Mitochondrial and Cytosolic Aspartate Aminotransferase.....Pages 111-116
Molecular Evolution of Alanine:Glyoxylate Aminotransferase Intracellular Targeting....Pages 117-122
Common Structural Elements in the Architecture of the Cofactor-Binding Domains in Unrelated Families of Pyridoxal Phosphate-Dependent Enzymes....Pages 123-128
Front Matter....Pages 109-109
Mitochondrial Localization of Eukaryotic NifS-Like Proteins....Pages 129-133
Mechanistic Studies Of 8-Amino-7-Oxononanoate Synthase.....Pages 135-142
Front Matter....Pages 143-143
Structure and Function of Tryptophan Synthase....Pages 145-150
Salt Bridging and Movalent Cation Binding Regulate Catalysis and Channeling in Tryptophan Synthase....Pages 151-156
Equilibrium Isotope Effects: Evidence for Low-Barrier H-Bonding in Tryptophanase....Pages 157-160
3-Dimensional Structures of Rabbit Cytosolic and E. Coli Serine Hydroxymethyltransferase....Pages 161-166
Role of Y65 and E57 in Escherichia coli Serine Hydroxymethyltransferase....Pages 167-170
Reaction and structure of 1-aminocyclopropane-1-carboxylate deaminase....Pages 171-174
Front Matter....Pages 175-175
Refined Reaction Mechanism of Aspartate Aminotransferase....Pages 177-182
 1 H and 15 N NMR Spectroscopy of Aspartate Aminotransferase and Related Schiff Bases and Tautomerism in Enzyme Active Sites....Pages 183-188
Structure, Induced Fit and Substrate Recognition of E. coli Branched-Chain Amino Acid Aminotransferase....Pages 189-194
Front Matter....Pages 195-195
Tryptophan Tryptophylquinone Enzymes: Structure and Function....Pages 197-202
Continuous-Flow Column Electrolytic Spectroelectrochemical Method for Determination of Protein Redox Potentials — Application to Quinoproteins....Pages 203-208
Enantioselectivity of PQQ-containing Alcohol Dehydrogenases: Kinetic, Thermodynamic, and Molecular Modeling....Pages 209-212
Structural Studies of a soluble monomeric Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5....Pages 213-218
Electron Transport Systems for Quinohemoprotein Type II Alcohol Dehydrogenase of Pseudomonas Putida HK5....Pages 219-224
Front Matter....Pages 225-225
Activation and transformation of cells induce translocation of ornithine decarboxylase (ODC) to the surface membrane....Pages 227-232
A partially folded conformation is not the only requirement for import of mitochondrial aspartate aminotransferase....Pages 233-238
Engineering of B 6 enzymes....Pages 239-244
Association of Newly Synthesized Mitochondrial Aspartate Aminotransferase with Cytosolic Factors....Pages 245-249
Front Matter....Pages 225-225
Expression and Purification of Serine Palmitoyltransferase....Pages 251-254
Front Matter....Pages 255-255
5-Aminolevulinate Synthase: Pre-Steady State Reaction and Functional Role of Specific Active Site Residues....Pages 257-263
Function of Pyridoxal-5’-Phosphate in Bacterial Phosphorylases....Pages 265-270
Inhibition and Structural Changes of O -Acetylserine Sulfhydrylase-A from Salmonella typhimurium Upon Binding Sulfate and Chloride Anions....Pages 271-276
Structure-Function Relationships of Porcine Pyridoxal Kinase....Pages 277-280
The Contribution of a Conformationally-Mobile, Active-Site Loop to the Reaction Catalysed by Glutamate Semialdehyde Aminomutase....Pages 281-284
The Reactions of Glutamate 1-Semialdehyde Aminomutase with (R) and (S) Enantiomers of a Novel, Mechanism-Based Inhibitor, 2, 3-Diaminopropyl Sulfate....Pages 285-288
Structure and Function of E. Coli Pyridoxine Phosphate Oxidase....Pages 289-292
Front Matter....Pages 293-293
Role of Branched Chain Aminotransferase Isoenzymes in the Central Nervous System....Pages 295-300
Modulation of Gene Expression by Vitamin B 6 ....Pages 301-306
Pyridoxal 5-Phosphate and Calcium Channels....Pages 307-314
GABA-aminotransferase, a target for antiepileptic drug therapy....Pages 315-318
Front Matter....Pages 319-319
Mouse Ornithine Decarboxylase: Structural Comparisons to Other PLP-Dependent Enzymes....Pages 321-326
Structural and mechanistic studies of Trypanosoma brucei ornithine decarboxylase....Pages 327-332
Mechanistic Analysis of Dialkylglycine Decarboxylase....Pages 333-338
Studies on an Active Site residue, E177, That Affects Binding of the Coenzyme in D-Amino Acid Transaminase, and Mechanistic Studies on a Suicide Substrate....Pages 339-346
Front Matter....Pages 347-347
Functional properties of immobilized pyridoxal 5’-phosphate-dependent enzymes probed by absorption microspectrophotometry....Pages 349-354
Characterization of Recombinant Porcine Pyridoxal Kinase using Surface Plasmon Resonance Biosensor Technique....Pages 355-358
Three-Dimensional Model Of The ATP-Binding Domain Of Pyridoxal Kinase....Pages 359-362
Structural Fluctuations of Pyridoxal Kinase: Effect of Viscogen Cosolvents....Pages 363-368
Front Matter....Pages 347-347
The Aspartate Aminotransferase Folding Intermediates Recognized by GroEL are Partially Folded Monomers that Bind Pyridoxal Phosphate....Pages 369-373
Back Matter....Pages 375-377