Basic Laboratory Methods for Biotechnology: Textbook and Laboratory Reference

Contents
Preface
Ancillary Materials
Acknowledgements
Authors
UNIT I: Biotechnology Is the Transformation of Knowledge into Useful Products
	1 Techniques to Manipulate DNA: The Root of the Biotechnology Industry
	2 The Biotechnology Industry Branches Out
	3 The Business of Biotechnology: The Transformation of Knowledge into Products
UNIT II: Introduction to Quality in Biotechnology Workplaces
	4 An Overview of Quality Principles in Biotechnology
	5 Quality in Research Laboratories
	6 Documentation: The Foundation of Quality
UNIT III: Safety in the Laboratory
	7 Introduction to a Safe Workplace
	8 Working Safely in the Laboratory: General Considerations and Physical Hazards
	9 Working Safely with Chemicals
	10 Working Safely with Biological Materials
UNIT IV: Math in the Biotechnology Laboratory: An Overview
	11 Basic Math Techniques
	12 Proportional Relationships
	13 Relationships and Graphing
	14 Descriptions of Data: Descriptive Statistics
UNIT V: Obtaining Reproducible Laboratory Measurements
	15 Introduction to Quality Laboratory Measurements
	16 Introduction to Instrumental Methods and Electricity
	17 The Measurement of Weight
	18 The Measurement of Volume
	19 The Measurement of Temperature
	20 The Measurement of pH, Selected Ions, and Conductivity
	21 Measurements Involving Light – Part A: Basic Principles and Instrumentation
UNIT VI: Laboratory Solutions
	22 Preparation of Laboratory Solutions – Part A: Concentration Expressions and Calculations
	23 Preparation of Laboratory Solutions – Part B: Basic Procedures and Buffers
	24 Solutions: Associated Procedures and Information
	25 Laboratory Solutions to Support the Activity of Biological Macromolecules
UNIT VII: Quality Assays and Tests
	26 Introduction to Quality Laboratory Tests and Assays
	27 Achieving Reproducible Results with Polymerase Chain Reaction Assays
	28 Measurements Involving Light – Part B: Assays
	29 Achieving Reproducible Immunoassay Results
UNIT VIII: Cell Culture and Reproducibility
	30 Introduction to Quality Practices for Cell Culture
	31 Culture Media for Intact Cells
UNIT IX: Basic Separation Methods
	32 Introduction to Filtration
	33 Introduction to Centrifugation
	34 Introduction to Bioseparations
UNIT X: Biotechnology and Regulatory Affairs
	35 Biotechnology and the Regulation of Medical and Food Products
	36 The Lifecycles and Regulation of Pharmaceutical Products
	37 The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products
	38 Quality Systems in a Regulated Production Facility
Appendix: Answers to Practice Problems
Acronyms
Glossary Terms
Index