Barley: Methods and Protocols

Front Matter ....Pages i-xi
 An Introduction to Barley: The Crop and the Model  (Wendy A. Harwood)....Pages 1-5
 Mutation Breeding in Barley: Historical Overview  (Ludmila Ohnoutkova)....Pages 7-19
 A Practical Guide to Barley Crossing  (William T. B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, Jerome D. Franckowiak)....Pages 21-36
 Barley Anther Culture  (Ludmila Ohnoutkova, Tomas Vlcko, Mentewab Ayalew)....Pages 37-52
 Isolated Microspore Culture in Barley  (Patricio Esteves, François J. Belzile)....Pages 53-71
 TILLING in Barley  (Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, Nils Stein)....Pages 73-94
 Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings  (Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, Fiona M. Doohan)....Pages 95-114
 Agrobacterium-Mediated Transformation of Barley Immature Embryos  (Alison Hinchliffe, Wendy A. Harwood)....Pages 115-126
 Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development  (Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Michał Słota, Dorota Swiergolik, Iwona Szarejko)....Pages 127-151
 Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy  (Marek Marzec, Michael Melzer)....Pages 153-166
 Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy  (Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, Isabelle Colas)....Pages 167-179
 Microarrays for High-Throughput Gene Expression Analysis of Barley  (Jenny A. Morris, Pete E. Hedley)....Pages 181-194
 Genome Engineering Using TALENs  (Goetz Hensel, Jochen Kumlehn)....Pages 195-215
 Creating Targeted Gene Knockouts in Barley Using CRISPR/Cas9  (Tom Lawrenson, Wendy A. Harwood)....Pages 217-232
 Genotyping-by-Sequencing on the Ion Torrent Platform in Barley  (Amina Abed, Gaétan Légaré, Sonia Pomerleau, Jérôme St-Cyr, Brian Boyle, François J. Belzile)....Pages 233-252
 DNA Methylation Analysis in Barley and Other Species with Large Genomes  (Karolina Chwialkowska, Urszula Korotko, Miroslaw Kwasniewski)....Pages 253-268
 High-Resolution RT-PCR Analysis of Alternative Barley Transcripts  (Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P. G. Calixto, Linda Milne et al.)....Pages 269-281
 Exome Capture for Variant Discovery and Analysis in Barley  (Micha Bayer, Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell et al.)....Pages 283-310
Back Matter ....Pages 311-313