Bacteriology Methods for the Study of Infectious Diseases

Cover
BACTERIOLOGY METHODS FOR THE STUDY OF INFECTIOUS DISEASES
Copyright
Acknowledgement
One - Fundamental skills for infectious disease research
	1.1 Introduction
		1.1.1 Health and safety
		1.1.2 Basic laboratory preparation, kit and consumables
		1.1.3 Pipettes
	1.2 Aseptic technique
	1.3 Common laboratory units
	1.4 Top tips for working safely in the lab
	1.5 Notes page
Two - Bacterial growth in solid and liquid media
	2.1 Introduction
	2.2 Media, culture conditions and nutritional requirements
		2.2.1 Isolation
	2.3 Differential and selective media
	2.4 Estimating cell number
	2.5 Direct enumeration (viable cells)
	2.6 Notes page
Three - Microscopy and staining
	3.1 Introduction
	3.2 Light microscopy
	3.3 Preparing slides
	3.4 Stains
	3.5 Using the light microscope
	3.6 Other types of microscopy
	3.7 Notes page
Four -
Antimicrobial testing
	4.1 Introduction
		4.1.1 Antibiotics and targets
		4.1.2 Disc diffusion
		4.1.3 Antibiotic strip tests
		4.1.4 Broth dilution
		4.1.5 Synergy
		4.1.6 Novel compound testing
	4.2 Notes page
Five -
Cell culture-based infection models
	5.1 Introduction
		5.1.1 Which species of bacteria are you working with and which is the best cell line to use?
		5.1.2 Cell culture and enumeration
		5.1.3 Viability and multiplicity of infection
		5.1.4 Bacterial attachment and internalisation/invasion
		5.1.5 Using cell culture-based infection models to support previous observations and plan the next experiments
	5.2 Notes page
Six -
Biofilm models to understand infectious diseases
	6.1 Introduction
		6.1.1 Does the bacterium form a biofilm?
		6.1.2 How many bacteria are in the biofilm and are they viable?
		6.1.3 Applications of simple static biofilm models
		6.1.4 Other types of static biofilm model
		6.1.5 Studying biofilms under conditions of flow
	6.2 Notes page
Seven -
Gene expression analysis
	7.1 Introduction
	7.2 Sample preparation and DNA extraction
	7.3 Primers
	7.4 Electrophoresis to analyse polymerase chain reaction products
	7.5 Quantitative polymerase chain reaction
	7.6 Troubleshooting
	7.7 Notes page
Eight -
Screening for common virulence traits
	8.1 Introduction
		8.1.1 Agar-based tests
		8.1.2 Non-agar plate-based assays
		8.1.3 Virulence genes
	8.2 Notes page
Nine-
Community composition studies
	9.1 Introduction
		9.1.1 Using culture methods to study microbial communities
		9.1.2 Using culture methods to assess rates of mutation within a microbial community
		9.1.3 Analysis of microbial communities using molecular techniques
		9.1.4 Considering the impact of environment
		9.1.5 Summary
	9.2 Notes page
Ten -
Invertebrate infection models
	10.1 Introduction
		10.1.1 Caenorhabditis elegans
		10.1.2 Galleria mellonella
			10.1.2.1 Experimental planning
			10.1.2.2 Experimental protocol
			10.1.2.3 Testing an antimicrobial treatment
		10.1.3 Kaplan–Meier analysis of data
		10.1.4 Example of information to be entered into spreadsheet to calculate Kaplan–Meier survival probability based on G. mellonell...
	10.2 Summary
	10.3 Notes page
References
	Webpages
Index
	A
	B
	C
	D
	E
	F
	G
	H
	I
	K
	L
	M
	N
	O
	P
	Q
	R
	S
	T
	U
	V
	W
	Z
Back Cover