Bacterial Polysaccharides: Methods and Protocols

Front Matter ....Pages i-xiii
 Customizable Cloning of Whole Polysaccharide Gene Clusters by Yeast Homologous Recombination  (Michael A. Liu, Peter R. Reeves)....Pages 1-14
 RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria  (Zhongrui Ma, Peng George Wang)....Pages 15-23
 Purification of Capsular Polysaccharide Complex from Gram-Negative Bacteria  (Carlos A. Alvarez, Brian A. Cobb)....Pages 25-35
 Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity  (Irina Sadovskaya, Yann Guérardel)....Pages 37-45
 Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria  (Alice Chateau, Olaf Schneewind, Dominique Missiakas)....Pages 47-57
 Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan, and Lipoarabinomannan  (Kathryn C. Rahlwes, Julia Puffal, Yasu S. Morita)....Pages 59-75
 Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry  (Hélène B. Pfister, Xiaowei Lu, Sameh E. Soliman, Pavol Kováč)....Pages 77-88
 Direct Conjugation of Bacterial Polysaccharides to Proteins by Squaric Acid Chemistry  (Peng Xu, Pavol Kováč)....Pages 89-98
 Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for Studies in Cytokine Production  (Siziwe Bebe, Tassos Anastassiades)....Pages 99-113
 Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides  (Ashley S. Brott, David Sychantha, Anthony J. Clarke)....Pages 115-136
 Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Crystallization  (Taylor J. B. Forrester, Lana El Osta, Joseph S. Lam, Matthew S. Kimber)....Pages 137-150
 Utilization of Fluorescently Tagged Synthetic Acceptor Molecules for In Vitro Characterization of a Dual-Domain Glycosyltransferase Enzyme, KpsC, from Escherichia coli  (Liam Doyle, Olga G. Ovchinnikova, Chris Whitfield)....Pages 151-159
 Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O Antigenic Polysaccharides in Enteric Bacteria  (Vladimir Torgov, Leonid Danilov, Natalia Utkina, Vladimir Veselovsky, Alexander Kocev, Inka Brockhausen)....Pages 161-174
 Characterization of Arabinosyl Transfer Reactions in the Biosynthesis of Mycobacterial Cell Envelope (Lipo)Polysaccharides  (Shiva Kumar Angala, Mary Jackson)....Pages 175-186
 Enzymatic Synthesis of Repeating Unit Oligosaccharides of Escherichia coli O104  (Inka Brockhausen, Diana Czuchry)....Pages 187-202
 Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolysaccharide Biosynthesis  (Faviola Tavares-Carreón, Xiang Ruan, Amy Ford, Miguel A. Valvano)....Pages 203-213
 Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides  (Tasnim Abukar, Nakita Buenbrazo, Bettina Janesch, Laura Kell, Warren Wakarchuk)....Pages 215-235
 Glycosyltransferase Activity Assay Using Colorimetric Methods  (M. Shafiqur Rahman, Wensheng Qin)....Pages 237-243
 In Vitro Characterization of a Multidomain Glycosyltransferase Using Fluorescently Tagged Synthetic Acceptors  (Danielle M. Williams, Olga G. Ovchinnikova, Chris Whitfield)....Pages 245-253
 Glycosyltransferase-Coupled Assays for 4-Epimerase WbpP from Pseudomonas aeruginosa  (Sulav Sharma, Carole Creuzenet, Kenneth F. Jarrell, Inka Brockhausen)....Pages 255-268
 Assaying Fucosidase Activity  (Zoë Anne Megson, Paul Messner, Christina Schäffer)....Pages 269-278
 In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis  (Rupa Nagar, Alka Rao)....Pages 279-296
 High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus  (Omar M. El-Halfawy, Eric D. Brown)....Pages 297-308
Back Matter ....Pages 309-312