Avian and reptilian developmental biology : methods and protocols

Front Matter ....Pages i-xii
 Some Thoughts on Experimental Design  (Claudio D. Stern)....Pages 1-8
Front Matter ....Pages 9-9
 Comparative Genomics as a Foundation for Evo-Devo Studies in Birds  (Phil Grayson, Simon Y. W. Sin, Timothy B. Sackton, Scott V. Edwards)....Pages 11-46
 A Step-by-Step Guide to Assemble a Reptilian Genome  (Asier Ullate-Agote, Yingguang Frank Chan, Athanasia C. Tzika)....Pages 47-67
 Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes  (Jilin Zhang, Jing Li, Qi Zhou)....Pages 69-85
 Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses Using the Chicken Skin Paradigm  (Yung-Chih Lai, Randall B. Widelitz, Cheng-Ming Chuong)....Pages 87-100
 Application of a CAGE Method to an Avian Development Study  (Ruslan Deviatiiarov, Marina Lizio, Oleg Gusev)....Pages 101-109
Front Matter ....Pages 111-111
 CRISPR/Cas9 in the Chicken Embryo  (Valérie Morin, Nadège Véron, Christophe Marcelle)....Pages 113-123
 Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development  (David Huss, Rusty Lansford)....Pages 125-147
 Lentiviral-Mediated Transgenesis in Songbirds  (Wan-chun Liu, Marian Hruska-Plochan, Atsushi Miyanohara)....Pages 149-165
 In Ovo Electroporation Methods in Chick Embryos  (Hidekiyo Harada, Minoru Omi, Harukazu Nakamura)....Pages 167-176
 Genetic Manipulation of the Avian Urogenital System Using In Ovo Electroporation  (Claire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler, Craig A. Smith)....Pages 177-190
 Enhancer Analyses Using Chicken Embryo Electroporation  (Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, Hisato Kondoh)....Pages 191-202
 Transgene Introduction into the Chick Limb Bud by Electroporation  (Shogo Ueda, Takayuki Suzuki, Mikiko Tanaka)....Pages 203-208
Front Matter ....Pages 209-209
 Chicken Induced Pluripotent Stem Cells: Establishment and Characterization  (Aurelie Fuet, Bertrand Pain)....Pages 211-228
 Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis  (Jae Yong Han, Bo Ram Lee)....Pages 229-242
 Handling of Gametes for In Vitro Insemination in Birds  (Shusei Mizushima, Mei Matsuzaki, Tomohiro Sasanami)....Pages 243-257
 In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells  (Wataru Yamashita, Toyo Shimizu, Tadashi Nomura)....Pages 259-265
Front Matter ....Pages 267-267
 Lifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian Development  (Raul E. Diaz Jr., Federica Bertocchini, Paul A. Trainor)....Pages 269-284
 Model Clades Versus Model Species: Anolis Lizards as an Integrative Model of Anatomical Evolution  (Thomas J. Sanger, Bonnie K. Kircher)....Pages 285-297
 The Feather Model for Chemo- and Radiation Therapy-Induced Tissue Damage  (Zhicao Yue, Benhua Xu)....Pages 299-307
 An Early Chick Embryo Culture Device for Extended Continuous Observation  (Hans-Georg Sydow, Tobias Pieper, Christoph Viebahn, Nikoloz Tsikolia)....Pages 309-317
 A Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote Brains  (Pei-Shan Hou, Takuma Kumamoto, Carina Hanashima)....Pages 319-334
 Somitogenesis and Axial Development in Reptiles  (Cindy Xu, Mariana B. Grizante, Kenro Kusumi)....Pages 335-353
 MicroCT Imaging on Living Alligator Teeth Reveals Natural Tooth Cycling  (Randall B. Widelitz, Alaa Abdelhamid, M. Khalil Khan, Amr Elkarargy, Cheng-Ming Chuong, Ping Wu)....Pages 355-362
Back Matter ....Pages 363-365