Applications of Nanomaterials in Proteomics (Nanostructure Science and Technology)

Preface
Contents
1 An Overview of Proteomics and Related Nanomaterials
	1.1 Introduction of Proteomics
		1.1.1 Concept Introduction
		1.1.2 Research Strategy in Proteomics
	1.2 Introduction of PTM Proteomics
		1.2.1 Concept Introduction
		1.2.2 Common PTMs
		1.2.3 Cross-Link of Glycosylation and Phosphorylation
		1.2.4 Research Methods in PTM Proteomics
	1.3 Introduction of Peptidomics
	1.4 Nanomaterials as Separation Media
		1.4.1 Nanomaterials in Ordinary Proteomics
		1.4.2 Nanomaterials in Phosphorylated Proteomics
		1.4.3 Nanomaterials in Glycosylated Proteomics
		1.4.4 Nanomaterials in Peptidomics
	References
2 Application of Nanomaterials to Separation of Low-Abundance Proteins
	2.1 Introduction
	2.2 Hydrophobic–Hydrophobic Interaction
		2.2.1 Graphene as Functional Group
		2.2.2 Other Carbon as Functional Group
		2.2.3 Alkyl Chain as Functional Group
		2.2.4 Polymer as Functional Group
	2.3 Metal Ion/Oxide as Functional Group
		2.3.1 Metal Oxide
		2.3.2 Metal Ion
	2.4 Hydrazide as Functional Group
	2.5 Specific Peptide as Functional Group
	2.6 Aptamer as Functional Group
		2.6.1 α-Thrombin
		2.6.2 Insulin
	2.7 MIPs as Functional Group
	References
3 Application of Nanomaterials to Separation of Phosphorylated Proteins
	3.1 Introduction
	3.2 IMAC-Based Nanomaterials
		3.2.1 Ti4+–IMAC
		3.2.2 Zr4+−IMAC
		3.2.3 Other IMAC
		3.2.4 Multi-IMAC
	3.3 MOAC-Based Nanomaterials
		3.3.1 Single MOAC
		3.3.2 Multiple MOAC
	3.4 Combination of IMAC and MOAC
		3.4.1 Physical Combination
		3.4.2 Chemical Combination
	3.5 Amine-Based Nanomaterials
		3.5.1 Guanidyl as Functional Group
		3.5.2 Hydrazide as Functional Group
		3.5.3 Polyethylenimine as Functional Group
		3.5.4 Urea as Functional Group
		3.5.5 Carbon Nitride as Functional Group
	3.6 Others
		3.6.1 4-(3-Acryloyl–Thioureido)–Benzoic Acid as Functional Group
		3.6.2 Fluorous Derivatization
		3.6.3 Epitope Imprinting
	References
4 Application of Nanomaterials to Separation of Glycosylated Proteins
	4.1 Introduction
	4.2 Hydrophilic Nanomaterials
		4.2.1 Carbohydrate as Functional Group
		4.2.2 Neutral Hydrophilic Polymer as Functional Group
		4.2.3 Amino Acid as Functional Group
		4.2.4 Zwitterionic Hydrophilic Polymer as Functional Group
		4.2.5 Iminodiacetic Acid as Functional Group
	4.3 Boronate Affinity Nanomaterials
		4.3.1 Small–Molecule Boronic Acid as Functional Group
		4.3.2 Boronic Acid Polymer as Functional Group
		4.3.3 Boronic Acid–Based MIPs
	4.4 Hydrazide–Based Nanomaterials
	References
5 Simultaneous Application of Nanomaterials to Separation of Phosphorylated and Glycosylated Proteins
	5.1 Introduction
	5.2 Hydrophilic MOAC
	5.3 Hydrophilic IMAC
	5.4 Combination of Boronic Acid and MOAC
	5.5 Smart Polymer
	References
6 Application of Nanomaterials to Separation of Endogenous Peptides
	6.1 Introduction
	6.2 Separation of Endogenous Ordinary Peptides
		6.2.1 Hydrophobic Mesoporous Silica
		6.2.2 Mesoporous Carbon/Silica–Carbon
		6.2.3 Hydrophobic COF
		6.2.4 Metal Ion as Functional Group
		6.2.5 Combination of Hydrophobicity and Metal Ion
	6.3 Separation of Endogenous Phosphopeptides
		6.3.1 Mesoporous IMAC
		6.3.2 Mesoporous MOAC
	6.4 Separation of Endogenous Glycopeptides/Glycans
		6.4.1 Hydrophilic Mesoporous Silica
		6.4.2 Hydrophilic MOF
		6.4.3 Hydrophilic COF
		6.4.4 Boronic Acid-Modified Mesoporous Silica
		6.4.5 Mesoporous Carbon/Silica–Carbon
	References
7 Conclusion
Index