Antibody Engineering: Methods and Protocols

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
 Antibody Design and Humanization via In Silico Modeling  (Vinodh B. Kurella, Reddy Gali)....Pages 3-14
 Antibody Affinity Maturation by Computational Design  (Daisuke Kuroda, Kouhei Tsumoto)....Pages 15-34
 Use of IMGT® Databases and Tools for Antibody Engineering and Humanization  (Marie-Paule Lefranc, François Ehrenmann, Sofia Kossida, Véronique Giudicelli, Patrice Duroux)....Pages 35-69
Front Matter ....Pages 71-71
 Construction of Human Naïve Antibody Gene Libraries  (Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, Katia Scotlandi)....Pages 73-91
 Construction of Synthetic Antibody Libraries  (Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau)....Pages 93-108
 Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments  (Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka et al.)....Pages 109-127
 Display Technologies for Generation of Ig Single Variable Domains  (Vladimir Bobkov, Bas van der Woning, Hans de Haard)....Pages 129-144
 A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries  (Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir et al.)....Pages 145-161
Front Matter ....Pages 163-163
 Phage Display and Selections on Purified Antigens  (Magali Colazet, Patrick Chames)....Pages 165-178
 Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display  (Martina L. Jones, Stephen M. Mahler, Sumukh Kumble)....Pages 179-195
 Selection of Antibody Fragments Against Structured DNA by Phage Display  (Mahdi Zeraati, Marcel E. Dinger, Daniel Christ)....Pages 197-209
 Selection of Antibody Fragments by Yeast Display  (Nathalie Scholler)....Pages 211-233
 Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display  (Birgit Dreier, Andreas Plückthun)....Pages 235-268
 In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display  (Naoto Nemoto, Shigefumi Kumachi, Hidenao Arai)....Pages 269-285
 Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood  (Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ et al.)....Pages 287-309
Front Matter ....Pages 311-311
 Expression of IgG Monoclonals with Engineered Immune Effector Functions  (Rodrigo Vazquez-Lombardi, Damien Nevoltris, Romain Rouet, Daniel Christ)....Pages 313-334
 An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High-Level Monoclonal Antibody Producing CHO DG44 Cell Lines  (Jessna H. M. Yeo, Mariati, Yuansheng Yang)....Pages 335-349
 Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins  (Martin Siegemund, Nadine Beha, Dafne Müller)....Pages 351-364
Front Matter ....Pages 365-365
 Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases  (Chao Li, Tiezheng Li, Lai-Xi Wang)....Pages 367-380
 Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity  (Christian Kellner, Stefanie Derer, Katja Klausz, Sophia Rosskopf, Tim Wirt, Thies Rösner et al.)....Pages 381-397
 Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High-Affinity Interaction with FcRn at pH 6.0  (Abhishek Saxena, Bingxin Bai, Shin-Chen Hou, Lianlian Jiang, Tianlei Ying, Shane Miersch et al.)....Pages 399-417
Front Matter ....Pages 419-419
 Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance  (Stephen Hearty, Paul Leonard, Hui Ma, Richard O’Kennedy)....Pages 421-455
 Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development  (Edward Franklin, Orla Cunningham, Brian Fennell)....Pages 457-477
 The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies  (Peter M. Bowers, William J. Boyle, Robert Damoiseaux)....Pages 479-489
 Selection and Use of Intracellular Antibodies  (Sandrine Moutel, Clément Nizak, Franck Perez)....Pages 491-503
 Site-Specific Radioactive Labeling of Nanobodies  (Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt et al.)....Pages 505-540
Back Matter ....Pages 541-543