Animal Cell Technology: Developments Towards the 21st Century

Front Matter....Pages i-xxx
Front Matter....Pages 1-1
Human Virus Detection Using Cells Immortalised by Oncogenes....Pages 3-7
Immortalised Calf Thyroid and Piglet Kidney Cell Lines for Foot and Mouth Disease Virus (FMDV) Isolation and Diagnosis....Pages 9-13
Establishment and Characterization of a Stable, Amplified Chinese Hamster Ovary (CHO) Cell Line Producing human Insulin-like Growth Factor Binding Protein-1 (hIGFBP-1)....Pages 15-19
Preliminary Studies With Scaleable Systems for the Production of Replication-Deficient Recombinant Adenoviruses in Mammalian Cell Lines....Pages 21-26
Cell Engineering to Optimise Protein Secretion: Analysis of Components of the Secretory System....Pages 27-31
Proliferation control of mammalian cells by the tumor suppressor IRF-1....Pages 33-43
Cultivation of Simian Virus 40 Large T Transfected Human Endothelial Cell Lines in Serumfree Medium....Pages 45-48
High Level Transient Expression in Mammalian Cells: Identification and Optimization of Physico-Chemical Parameters of the Calcium Phosphate Transfection Method....Pages 49-55
Immortalization of Human Endothelial Cells by Simian Virus 40....Pages 57-61
Identification and Analysis of The Hepatocyte Growth Factor Activator....Pages 63-67
Production of SeNPV in a Suspension of an Established Spodoptera Exigua Cell Line....Pages 69-72
The Use of Very Low Multiplicities of Infection with the Baculovirus Expression Vector System....Pages 73-77
Oncogene Activated Production System to Enhance Cellular Recombinant Protein Productivity....Pages 79-84
Transfer of SV40 - T Expression Vectors into Human Monocytes....Pages 85-90
 RAS Oncogene Enhances the Various Recombinant Protein Productivities of BHK-21 Cells Regulated by the CMV Promoter....Pages 91-95
Production of Recombinant Prourokinase by CHO Cells in a Perfusion Biosilon Microcarrier Culture System....Pages 97-101
Front Matter....Pages 103-103
Influence of the Culture System Upon Growth and Productivity of Animal Cells in Stirred Tanks....Pages 105-109
Determination of Metabolic Fluxes in Animal Cells....Pages 111-115
Comparison of Nutrient Requirements of Mammalian Cell Lines....Pages 117-123
The Effect of Unsaturated Fatty Acids on the Growth, Metabolism and Productivity of a Murine Hybridoma....Pages 125-129
Front Matter....Pages 103-103
Infection of the Insect Cells....Pages 131-135
Effect of Environmental Conditions on Intracellular pH of Hybridoma Cells Cultured in Bioreactors....Pages 137-141
Effect of Sodium Butyrate on Protein Production in Different Culture Systems....Pages 143-147
Human Therapeutic Monoclonal Anti-D Antibody Produced in Long-Term Hollow-Fibre Culture....Pages 149-153
High-Density Cytostat Cell Cultures with Homeostasis of Glucids and Amino Acids....Pages 155-161
Increase of Productivity in Recombinant Cho-Cells by Enhanced Glucose-Levels....Pages 163-167
Continuous Fermentation Consumption Kinetics for Basal Media Design....Pages 169-174
Effect on Cultured Human Cell Lines of Polysaccharides in the Stem of Kiwi Fruit....Pages 175-179
Serum-Free Monoclonal Antibody Production in a Hollow Fibre Bioreactor....Pages 181-186
Media Additives for High Performance Cell Culture in Hollow Fiber Bioreactors....Pages 187-191
Formate Production by CHO Cells: Biosynthetic Mechanism and Potential Cytotoxicity....Pages 193-197
Productivity Enhancement Using Liquid Medium Concentrates....Pages 199-204
Serum-Free Media: Influence of The Definition of The Cell Culture Environment on Stability and Antibody Productivity of Mouse Hybridomas....Pages 205-209
Influence of the Temperature on Process Optimisation....Pages 211-215
The Effects of Initial Serum Concentration, pH and Agitation Rate on Cell Aggregation During Batch Culture of Chinese Hamster Ovary (CHO) Cells....Pages 217-221
Long-Term Serum/Plasma-Free Culture of Human Cytotoxic T Lymphocytes Induced from Periphral Blood Mononuclear Cells....Pages 223-227
Specific Growth Rate as a Parameter for Tracing Growth Limiting Substances....Pages 229-233
Influence of Glucose, Glutamine and Oxygen on the Metabolism of Hybridomas and Peripheral Blood Cells....Pages 235-239
Ammonium Transport in Myeloma and Hybridoma Cells....Pages 241-245
Effects of ammonium ion removal on growth and MAb production of hybridoma cells....Pages 247-262
Front Matter....Pages 103-103
Evaluation of the New Protein-Free Powder Medium (MDSS1-Powder) for the Production of Monoclonal Antibodies....Pages 263-270
Cell Growth Regulatory Peptides from Bovine Casein....Pages 271-275
Optimizing Vendor Proprietary Serum-Free Media....Pages 277-281
Metabolic Effects of Substrate Limitation on Insect Cells....Pages 283-287
Cell-Cycle Phase Analysis in Glucose-Limited Continuous Cultivation for the Effective Production of Antibody....Pages 289-294
Hyperosmotic Hybridoma Cell Cultures: Mechanisms of Osmoprotection....Pages 295-299
Investigation of High Density Cell Culture Kinetics Using On-Line Cell Density and Metabolic Rate Measurements....Pages 301-305
The Effect of Medium Composition on Growth and Monoclonal Antibody Production of Hybridoma Cells....Pages 307-312
Investigations of high cell density baculovirus infection using Sf9 and High Five insect cell lines in the low-cost SF-1 medium....Pages 313-315
Large scale production of Mammamodulin Development of a low-cost large scale production process for adherent cell growth....Pages 317-319
Transcription Level of CHO Cells Influenced by Amino Acids....Pages 321-325
Culture Conditions for High Performance Production of Monoclonal Antibody....Pages 327-332
Expression of heterologous proteins in CHO DUKX B11 cells pre-adapted to growth in serum-free suspension culture....Pages 333-337
In Situ Ammonium Extraction in a Continuous Medium Recycling Bioreactor for the Production of Cell Growth inhibitors....Pages 339-343
Growth of Anchorage-Dependent Mammalian Kidney Cells in Serum-Free Media....Pages 345-348
Performance Characteristics of a Protein-Free Medium for Mammalian Suspension Cells....Pages 349-356
Simultaneous Prediction of the Concentrations of Glucose, Glutamine, Lactic acd, Ammonia and Antbody in the Culture Broth of Mouse–Mouse Hybrdoma by Near Nfrared Reflectance Spectroscopy....Pages 357-361
Front Matter....Pages 363-363
Serum Content Affects the Structure and Activity of the Antibody Produced by Animal Cells in Culture....Pages 365-369
Protein folding in the secretory pathway of animal cells....Pages 371-376
Effects of Ammonia and Glucosamine on the Glycsosylation Pattern of Recombinant Proteins Expressed from BHK-21 Cells....Pages 379-384
Front Matter....Pages 363-363
Production of Recombinant Human Thyroid Peroxidase in Chinese Hamster Ovary Cells....Pages 385-389
Effect of Lipids on Recombinant Interferon-γ Glycosylation....Pages 391-396
Production and Post-Translational Modification of Tissue Plasminogen Activator (tPA) in Namalwa Cells....Pages 397-401
The Role of Physiological Cell Parameters and Culture Conditions on Product Consistency and Glycosylation Pattern of Recombinant CHO Expressed Glycoproteins - Interferon Omega and Tissue Plasminogen Activator....Pages 403-407
Identification of Carbohydrate Structures of Glycoproteins by a Novel Method Utilizing a Lectin-Elisa....Pages 409-413
Human Therapeutic Monoclonal Anti-D Antibody Produced in Long Term Hollow Fibre Culture....Pages 415-419
Sialidase Activity in Culture Fluid of Chinese Hamster Ovary Cells During Batch Culture and Its Effect on Recombinant Glycoprotein Integrity....Pages 421-425
Characterisation And Analysis Of Human Interferon-Y Glycoforms Produced In Baculovirus Infected Spodoptera Frugiperda (SF9) And Estigmena Acrea (EA) Cell Lines ....Pages 427-430
Erythropoietin processing in erythropoietic system and central nervous system....Pages 431-441
Generation of Affinity-Variant Antibodies via the Alteration of Glycosylation in Light Chain Effected by Defined Culture Conditions of Human Hybridomas....Pages 443-447
Production, Purification and Application of Flatfish (Paralichthys olivaceus) Interferon....Pages 449-453
Rapid HPLC Quantification of Recombinant Human Antithrombin III during Production and Purification....Pages 455-459
Front Matter....Pages 461-461
Cloning of the Rearranged Variable Regions of Mouse Immunoglobulin Heavy and Light Chain Genes Using the Polymerase Chain Reaction....Pages 463-468
Production of a Biologically Active Chimeric Mouse/Human Monoclonal Antibody in Insect Cells....Pages 469-473
Monoclonal Antibodies Against Human Osteocalcin Made by Using Recombinant GST-rhOC as an Immunogen....Pages 475-479
Functional display of proteins, mutant proteins, fragments of proteins and peptides on the surface of filamentous (bacterio) phages: A review....Pages 481-487
Establishment of Antigen Primed Two-and Three-Dimensional Cultures of Human Lymphoid Cells for the Development of Efficient in Vitro Immunization Protocols....Pages 489-493
Comparison of Different Bioreactors and Media for the Production of Specific Monoclonal Iga Antibodies....Pages 495-499
Immunoglobulin Production Stimulating Activities of Nucleic Acid-Binding Proteins....Pages 501-507
B cell activation by plasma membranes from activated helper T cells....Pages 509-514
Front Matter....Pages 461-461
Purification of Monoclonal Antibodies by Fluidized Bed Adsorption....Pages 515-519
Construction and Expression of Chimeric Protein A - Marine Firefly Luciferase in Mammalian Cells....Pages 521-526
Front Matter....Pages 527-527
Human Therapeutic Monoclonal Anti-D Antibody Produced in Long-Term Hollow-Fibre Culture....Pages 529-533
Variation in BHK Cell Populations, Examined by Multi-Locus DNA Fingerprinting....Pages 535-537
Manufacturing of BioPharmaceuticals....Pages 539-545
Removal of DNA in Cell Cultured Fluid Using BMM TM ....Pages 547-551
Facility Design and Validation Considerations for Continuous Cell Culture Processes....Pages 553-559
Significance of Specific Productivity for the Effectiveness and Consistency of Mammalian Cell Cultivation....Pages 561-565
Advantages and disadvantages of glucose limitation in perfused mammalian cell cultures....Pages 567-573
Stability of Chimeric Antibody Producing Transfectomas during a Long-Term Culture....Pages 575-579
Acceptability of continuous cell lines for the production of biologicals....Pages 581-587
Theoretical Requirements for The Integrity Test Method and Its Practical Application....Pages 589-595
Excellent Virus Removal on The Filtration of Biotechnology Product Solution by Planova Filter....Pages 597-603
The acceptability of continuous cell lines: A personal & historical perspective....Pages 605-609
Development of Procedures for Inactivating Microorganisms from Protein Components....Pages 611-617
A Reliable Strategy for The Achievement of Cell Lines Growing in Protein-Free Medium....Pages 619-623
Inactivation of Hepatitis a Virus....Pages 625-629
Viral validation strategy for recombinant products derived from established animal cell lines....Pages 631-635
Filtration of PrP....Pages 637-640
A Simple and Rapid Method for Detection of Mycoplasmas in Mammalian Cell Cultures....Pages 641-646
Front Matter....Pages 527-527
Cultivation and Downstream Processing of a Human Monoclonal Anti-Alpha Haemolysin Antibody for the Treatment of Septic Patients....Pages 647-651
Front Matter....Pages 653-653
Hybridomas in a Bioreactor Cascade for Kinetic Parameter Determination....Pages 655-659
Cellgrowth and Viruspropagation in the Costar Cellcube System tm ....Pages 661-663
Model-Based Monitoring and Control of a Monoclonal Antibody Production Process....Pages 665-670
Evaluation of a Cell Separator in Large Scale Perfusion Culture....Pages 671-675
Carbon Dioxide Evolution Rates in Animal Cell Culture in Bicarbonate Buffered and Bicarbonate Free Medium....Pages 677-681
Production of Monoclonal Antibodies in a Pilot Scale Fluidized Bed Bioreactor....Pages 683-686
The Use of Software Sensors for Measurement and Control of Animal-Cell Cultures....Pages 687-691
Finite Element Analysis of 3-D Flow in an Agitated Bioreactor....Pages 693-697
Scale-Up of Ultrasonic Resonance Field Cell Separation Devices Used in Animal Cell Technology....Pages 699-703
Fast Propagation of Hybridoma Cells in a Forced-Flow Membrane Perfusion Reactor....Pages 705-710
Implications of Controlled Glycosylation and Apoptosis in Novel Hollow Fiber Bioreactor....Pages 711-715
The Effect of Flow Rate on the Immobilisation of Anchorage Independent Cells to Porous Glass Beads....Pages 717-721
Immobilisation of Anchorage-Independent Cells to Porous Glass Beads: Mass Transfer Phenomena in Long - Term Packed Bed Reactor....Pages 723-728
Continuous Biotransformation by Immobilized Microorganism on the Surface of Cuprammonium Regenerated Cellulose Hollow Fiber (BMM)....Pages 729-734
Determination of K s -Values (Monod Constants) for Essential Amino Acids Using CHO and Hybridoma Cells in Batch and Chemostat Cultures....Pages 735-739
Cell Culture Application of a Nutating Disk Pump....Pages 741-745
Small Microcarrier Aggregates Yield High Cell Density....Pages 747-751
Testing Computer Predictions of Viral Effects on Hybridoma Productivity....Pages 753-757
Subculture Method for Large Scale Cell Culture Using Macroporous MicroCarrier....Pages 759-763
Front Matter....Pages 653-653
Mab Production and Treatment of Rheumatoid Disease Using Encapsulated Anti–Idiotypic Hybridoma Cells....Pages 765-769
Expression of Recombinant Human Interleukin-2 in Baby Hamster Kidney Cells and Cultivation on Different Carriers....Pages 771-775
Detachment and Mitosis of Adherent Cells under Shear Stress Conditions....Pages 777-781
Evaluation of a Porous Silicone Carrier (Immobasil G) for Animal Cell Culture....Pages 783-786
Patterns of the specific productivity of the murine hybridoma, AFP-27, in batch culture....Pages 787-791
Formation and Disruption Mechanisms of Animal Cell Aggregates....Pages 793-797
BHK Aggregate Sedimentation: Cell Concentration and Downstream Processing....Pages 799-803
Growth of BHK Aggregates in A 2 Litter Bioreactor....Pages 805-809
Bioreactor with a Radial-Flow Non-Woven Fabric Bed for Animal Cell Culture....Pages 811-815
Production of Human Growth Hormone by Protein-free Cultivation of Anchorage-dependent Cells with Porous Cellulose Carrier....Pages 817-821
Application of Dialysis Techniques for Effective Production of Biologicals with Immobilited Animal Cells....Pages 823-827
Evaluation of Process Strategies for Efficient Cultivation of Hybridoma Cells Based on Mathematical Models....Pages 829-833
High Density Culture of Animal Cells Using Macroporous Cellulose Carriers....Pages 835-839
Comparative Study for the Propagation of Anchorage- Dependent Cells Using Different Forms of Macroporous Carriers....Pages 841-845
Monitoring and Modelling Hybridoma Cultures....Pages 847-850
Control of Cell Concentration in Fedbatch Hybridoma Cultures....Pages 851-854
Improvement of mammalian cell fed-batch culture....Pages 855-857
On-Line Monitoring and Control of Bioprocesses Using an Object Oriented Language with Graphical User Interfaces....Pages 859-864
Non-Invasive On-Line Investigations of Industry Style Bioreactors....Pages 865-869
Aggregate Animal Cell Culture by Using Microbeads....Pages 871-875
Front Matter....Pages 653-653
IL-4 Production Using Macroporous Microcarrier....Pages 877-881
Oxygen gradients in animal-cell bioreactors....Pages 883-891
Bubble Bed Aeration for Animal Cell Cultures....Pages 893-898
Microcarrier technology, present status and perspective....Pages 899-905
Cultivation of Animal Cells in a New Modular Minifermenter....Pages 907-913
Large Scale Suspension Perfusion Culture Process with a Compact Centrifuge for Mammalian Cell Recycle....Pages 915-919
Front Matter....Pages 921-921
Model System for Apoptotic Death in Cell Cultures....Pages 923-925
Alteration of Apoptotic Cell Death by Genetic Modulation....Pages 927-932
Bioassay Method for Glycoalkaloids in Food with Animal Cell Cultures....Pages 933-937
Organotypical Construction of a Hybrid Artificial Liver....Pages 939-943
Transfer and expression of the human multiple drug resistance gene as potential human gene therapy....Pages 945-951
Determination of Extracellular Ganglioside G M3 in Hybridoma Cultures during Continuous-Medium-Recycling....Pages 953-957
Growth and Structure of Corneal Tissue in Simulated Microgravity....Pages 959-963
Apoptosis and nutrition: Involvement of amino acid transport system in repression of hybridoma cell death....Pages 965-971
Monitoring of immunotherapy with cytokines or monoclonal antibodies....Pages 973-986
Threedimensional Cultures of B-Chronic Lymphatic Leukaemia Cells Derived from Human Bone Marrow and Peripheral Blood in a Miniaturized Hollow Fibre Bioreactor....Pages 987-991
Differentiation of Human Leukemia Cells by Bacterial Extracellular Glycolipids....Pages 993-997
Cytotoxicity Testing for Food Safety Evaluation....Pages 999-1003
The Function of Glycosphingolipids as Revealed by Endoglycoceramidase Applications....Pages 1005-1009
The 31 KD Cytosolic Phospholipase A2 is a Target Molecule for the Cancer-Specific Human Monoclonal Antibody, AE6F4....Pages 1011-1015
Front Matter....Pages 921-921
Multicellular Hepatocyte Spheroids for Development of Bioartificial Liver Support System and Drug Metabolism Studies....Pages 1017-1023
Immunomodulation of Prodigiosin 25-C and Concanamycin B....Pages 1025-1029
The New Catalogue of the Cell Line Data Base....Pages 1031-1035
A Computer Integrated Control System for Specialized Cell Culture Fermentation Set-Ups....Pages 1037-1041
Detection of Micro-Metastasis by Polymerase Chain Reaction (PCR).....Pages 1043-1047
Development of a Hybrid Artificial Liver Support System Using Puf/Spheroid Packed-Bed....Pages 1049-1053
Deregulation of Control of Growth and Apoptosis Occurs with Age or Exposure to Certain Mutagens in Normal Human Cells; A Possible Predictive Test for Cancer Risk....Pages 1055-1059
A Rapid Cytotoxicity Assay of Metabolic Inhibitors....Pages 1061-1065
Innate Biodefense Activation against Virus Infection by Sphingolipids in Foods....Pages 1067-1071
Replating of bioreactor expanded human bone marrow results in extended growth of primitive and mature cells....Pages 1073-1081
 Ex vivo expansion of primitive hematopoietic cells for cellular therapies: An overview....Pages 1083-1098
Functional Expression and Characterization of the Rat GABA A Receptor Subtype α 5 β 2 γ 2 in Sf 9 Insect Cells....Pages 1099-1103
Rapid and Large-Scale Preparation of Porcine Hepatocyte Spheroids and Their Functions in Continuous Suspension Culture....Pages 1105-1110
Growth Inhibition and Heavy Metal Accumulation in CHO Cells....Pages 1111-1115
Neuronal Cell Cultures and Digitized Fluorescent Imaging of Intracellular Calcium as Tools for in Vitro Drug Screening....Pages 1117-1121
Device for Cell Culture Fluid Sampling from Lab Scale Bioreactors....Pages 1123-1125
Metabolism of Confluent Vascular Endothelial Cell Cultures....Pages 1127-1131
Evaluation of Erythrocin B as a Substitute for Trypan Blue....Pages 1133-1139
Elucidation of Physiological Functions of Food Using Mammalian Cell Culture....Pages 1141-1145
Protein-Free Culture of a Human Macrophage Like UM Cell Line....Pages 1147-1151
Front Matter....Pages 921-921
Monitoring of Apoptotic Death in Hybridoma Cells by Flow Cytometry....Pages 1153-1157
Isolated Hepatocyte Transplantation(Tx)....Pages 1159-1165
Batch Control System Vaccines: BCSV....Pages 1167-1175
Characterization and Application of Ku-812-F, an Immature Human Basophilic Leukemic Cell Line....Pages 1177-1181
Induction of Apoptosis in an Intestinal Epithelial Cell Line, IEC-6....Pages 1183-1187
On-Line Monitoring and Control of Fed-Batch Culture of Hybridoma with the Aid of an Expert System....Pages 1189-1194
Back Matter....Pages 1195-1207