Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Fourth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 13–15 November 1991

Front Matter....Pages i-xiii
The Value of Models in Animal Cell Biotechnology....Pages 1-5
The Manzanar Project — A Unique Way to End World Hunger....Pages 7-8
Experience in Cell Line Testing....Pages 9-14
Virus Removal and Inactivation in Process Validation....Pages 15-30
Multilocus DNA Fingerprinting in the Analysis of Cell Stocks: Stability Studies and Application to a Wide Range of Species....Pages 31-39
Alteration of Cell Surface Glycoproteins through Differentiation from a Human Histiocytic Lymphoma Cell Line, U-937, to a Macrophage-Like Cell Line, U-M....Pages 41-47
Removability of Virus Particles and Permeability of Protein from Cell Culture Medium Using Cuprammonium Regenerated Cellulose Hollow Fiber (BMM™)....Pages 49-55
An Evaluation of Hybridoma Cell-Specific Productivity: Perfused Immobilized, Continuous Suspension, and Batch Suspension Cultures....Pages 57-63
Dynamics of Aggregate Culture of Mammalian Cells....Pages 65-69
Effect of Protein Adsorption on Cell Attachment on Solid Surfaces....Pages 71-76
Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriers....Pages 77-80
Application of Three Dimensional Culture of Adult Rat Hepatocytes in PUF Pores for Artificial Liver Support System....Pages 81-86
Novel Validation Method of Virus-Removability for Biological Cell Culture Products Using Polymeric Membrane Filters....Pages 87-102
Selective Formation and Immobilization by Re-Attachment of Floating Spheroids of Rat Hepatocytes....Pages 103-108
Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Production....Pages 109-114
Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture System....Pages 115-120
Oscillatory Behavior in Continuous Aggregate Cultures of Mammalian Cells....Pages 121-127
Detection of Mammalian Cell Attachment and Lysis on a Metal Surface Using a Piezoelectric Sensor....Pages 129-134
Strategies for Economic GMP Production of Mammalian Cell Expressed Proteins....Pages 135-141
Use of Medium Concentrates to Improve Bioreactor Productivity....Pages 143-148
Separation of animal cells in continuous cell culture systems....Pages 149-154
An Automated System for Hollow Fiber Cell Culture....Pages 155-164
Automatic Selection System of Monoclonal Antibody Producing Cells....Pages 165-171
Continuous Monoclonal Antibody Production by a Composite Gel Perfusion Bioreactor in a Protein-Free Medium....Pages 173-178
High Density Cultivation of Hybridoma Cells by Using Permeable Capillaries....Pages 179-184
Recognition and Counting Method of Mammalian Cells on Micro-Carrier Using Image Processing and Neural Network....Pages 185-191
Mitochondrial Genome Transcription in Cultured Animal Cells....Pages 193-200
Large-Scale Mammalian Cell Cultures by Application of Macroporous Cellulose Carriers....Pages 201-207
High Density Perfusion Culture of Animal Cells Using a Novel Continuous Flow Centrifuge....Pages 209-216
Membrane Dialysis Bioreactor with Integrated Radial-Flow Fixed Bed — A New Concept for Continuous Cultivation of Animal Cells....Pages 217-223
Effect of Glutamine Supplementation on the Growth of Hybridoma Cells Cultivated in a Reactor Coupled with an Ammonia Removing System....Pages 225-231
Virus Containment Continuous Culture of Animal Cells....Pages 233-237
A Novel Bioreactor Process for Anchorage-Independent Animal Cells Immobilized Within Porous Biomass Support Particles....Pages 239-244
Effects of Environmental Parameters on CHO Cell Growth in Batch and Chemostat Cultures and on Human IFN-γ Synthesis and Fidelity....Pages 245-249
Design and Characterization of Electrically Controlled Bioreactor.....Pages 251-256
Modulation of the Proliferation of Cultured Hepatoma Cells by Urea Cycle-Related Amino Acids....Pages 257-263
The Effects of Glutamine Concentration on Growth and Monoclonal Antibody Production in Fed-Batch Operation....Pages 265-270
Stimulation of Animal Cell Metabolism by Shear Stress....Pages 271-277
Effects of Conditioned Medium on the Growth Kinetics and the Monoclonal Antibody Productivity of Hybridoma Cells....Pages 279-285
Factors influencing expression and post-translational modification of recombinant protein C....Pages 287-293
A Simple Automatic Control System of pH and DO....Pages 295-302
Hybridoma Culture in Iron-Rich Protein-Free Medium: Cell Growth, Apoptosis, and Nature of Macromolecules Recovered from the Culture Fluid....Pages 303-309
A Novel Vero Cell Line for Use as a Mammalian Host-Vector System in Serum-Free Culture....Pages 311-319
Serum Free Culture of Human-Human Hybridoma Secreting Monoclonal Antibodies Against Stomach Cancer....Pages 321-326
Serum-Free Media for the Growth and Maintenance of the Differentiated State of Keratinocytes, Hepatocytes, and Neural Cells....Pages 327-334
Characterization of an Airway Epithelial Cell from Neonatal Rat....Pages 335-343
Production of Human Antithrombin-III in a Serum-Free Culture of CHO Cells....Pages 345-351
A Novel Protein- and Hormone-Free Medium: Its Application to Hybridoma Culture and Monoclonal Antibody Production....Pages 353-359
EDF (Erythroid Differentiation Factor = Activin A) Production in Recombinant CHO Cells....Pages 361-367
A Novel Serum Free Medium for Mouse Hybridomas and Human Lymphoblastoid Cell Lines....Pages 369-374
A Simple Structured Model Predicted Positively-, Negatively-, or Non-Growth Associated Antibody Production Rate Depending on Culture Conditions....Pages 375-381
Change in Monoclonal Antibody Productivity in Immobilized Hybridoma Cells at Low Dissolved Oxygen Concentrations....Pages 383-389
Improved Monoclonal Antibody Production of a Hybridoma by Varying the Oxygen Tension during a Suspension Culture....Pages 391-396
Enhancement of Productivity by Yield Improvements Using Simulation Techniques....Pages 397-403
Studies on the Production of Recombinant Human NF-IL6 in Mouse L Cell....Pages 405-412
Enhancing Effect of Interleukin-6 on Antibody Productivity of a Hybridoma Cell Line....Pages 413-418
Improved Production of Recombinant Proteins by the Baculovirus Expression System....Pages 419-424
Production and Product Integrity of Recombinant HIV-1 gag -Particles (p55) with the Baculovirus Expression System....Pages 425-430
Enhancement of Biological Productivity in Serum-Free Culture....Pages 431-438
Enhancement of IgM Production of HB4C5 Cells by Chitosan....Pages 439-443
Repression of Ovalbumin Gene Expression in the Chicken Oviduct Cell....Pages 445-451
Constitutive Long-Term Production of Recombinant Human Interferon-Gamma by Transformed Mouse C127 Cells Cultured in Serum Free Medium....Pages 453-462
Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Targeting in Filter-Grown Madin-Darby Canine Kidney (MDCK) Cells....Pages 463-471
Protein Tyrosine Sulfation in Animal Cells and its Simulation in Vitro in the Downstream Processing of Proteins....Pages 473-479
Expression of Carp α-Globin Gene in Tilapia Ovary Cells....Pages 481-489
Immunosuppressive Functions of Cells from Intestinal Tissues....Pages 491-497
Biological Functions of Carbohydrates in Erythropoietin....Pages 499-507
Cloning and Expression of Flatfish Interleukin 2 Gene....Pages 509-514
Serum-Free Mouse Embryo (SFME) Cells and Transforming Growth Factor (TGF)β1....Pages 515-519
Anti-Tetanus Human Monoclonal Antibodies-Comparative Studies on their Protective Activity with Polyclonal Antibody....Pages 521-525
Production of chicken monoclonal antibody....Pages 527-534
Generation and Characterization of Murine Anti-Human Transforming Growth Factor-β1 Monoclonal Antibodies....Pages 535-539
Examination of lung cancer using a human monoclonal antibody HB4C5-clone 3....Pages 541-545
Alteration of Reactivity of Human Monoclonal Antibodies Produced by Concanavalin A-Resistant Hybridomas....Pages 547-551
Development of Mouse Fibroblast Dermal Equivalent Using Tissue Culture Technique....Pages 553-558
Effects of Some Food Additives and Mycotoxins on the Growth of HuH-6KK Cells....Pages 559-563
Europium-Linked Immuno Assay: Production of Immunogloblin by Human-Human Hybridoma Cell Lines....Pages 565-570
The Protective Role of Polyphenols in Cytotoxicity of Hydrogen Peroxide....Pages 571-576
Back Matter....Pages 577-584