Animal Cell Technology. Developments, Processes and Products

Content: 
Front Matter, Page iii
Copyright, Page iv
Organizing Committee, Page v
Sponsors, Page vi
ESACT Executive Committee, Page vii
11th ESACT Meeting, Brighton, United Kingdom, Page viii
CHAIRMAN'S STATEMENT, Pages ix-xii, R.E. Spier
THE CHARACTERIZATION OF SV40-IMMORTALIZED MURINE MACROPHAGE CELL LINES, Pages 3-5, Ute Kreuzburg-Duffy, Caroline MacDonald
IDENTIFICATION OF TYPE-D RETROVIRUSES AS CONTAMINANTS IN WIDELY USED CELL LINES, Pages 6-8, D. Morgan, K.T. Smith, H.M. Laird, G.M. Lees
CHARACTERIZATION OF HUMAN ENDOTHELIAL HYBRID CELLS EXPRESSING VON WILLEBRAND FACTOR, Pages 9-11, Otmar Hohenwarter, Christine Schmatz, Hermann Katinger
PRIMARY CULTURES OF HUMAN PROXIMAL TUBULE CELLS (HPTC) IN SERUM FREE DEFINED MEDIA: A CHARACTERIZATION STUDY, Pages 12-14, Françoise Courjault, Jacques Chevalier, Danielle Leroy, Dominique K. Chopin, Clément C. Abbou, Hervé Toutain
CULTURE OF CELLS FROM ADULT ZEBRAFISH (BRACHYDANIO RERIO) AND EMBRYO, Pages 17-19, Yuto Kamei, Paul Collodi, Ted Ernst, David W Barnes
CLONING OF MOUSE HYBRIDOMA CELLS INFECTED WITH MYC AND RAS-CONTAINING RETROVIRUSES YIELDS CELL LINES WITH IMPROVED GROWTH AND ANTIBODY PRODUCTION, Pages 20-22, Chris Darnbrough, Patricia Watts, Caroline MacDonald
SURVIVAL OF B LYMPHOCYTES IS PROLONGED AFTER INFECTION WITH IMMORTALIZING VIRUSES, Pages 23-25, Wendy Filsell, Susan A. Bayley
ESTABLISHMENT OF DERMAL PAPILLA CELL LINES BY RETROVIRUS-MEDIATED TRANSFER OF ONCOGENES, Pages 26-28, Amanda J. Stones, Wendy Filsell, Susan A. Bayley
IMMORTALISATION OF FLATFISH (PARALICHTHYS OLIVACEUS) LEUKOCYTES BY ONCOGENE TRANSFECTION, Pages 29-31, Tadakazu Tamai, Nobuyuki Sato, Shoji Kimura, Sanetaka Shirahata, Hiroki Murakami
Host Cell Derived Retroviral Sequences Enhance Transfection and Expression Efficiency in CHO Cells, Pages 35-41, F.M. Wurm, A. Johnson, Y. Lie, T. Etcheverry, K.P. Anderson
STABILITY OF PRODUCTION OF RECOMBINANT ANTIBODIES FROM GLUTAMINE SYNTHETASE AMPLIFIED CHO AND NS0 CELL LINES, Pages 42-47, T. Hassell, H. Brand, G. Renner, A. Westlake, R.P. Field
THE RAPID PRODUCTION OF RECOMBINANT RABBIT METALLOPROTEINASES IN MYELOMA CELLS, Pages 48-50, C.M. Gofton, G. Roberts, S. Bergin, R.J. Owens
LONG TERM STABILITY OF EXPRESSION OF HUMANIZED MONOCLONAL ANTIBODY CAMPATH 1-H IN CHINESE HAMSTER OVARY CELLS, Pages 51-53, J Raper, Y Douglas, N Gordon-Walker, C A Caulcott
ONCOGENE-ACTIVATED PRODUCTION OF RECOMBINANT PROTEINS FOR ANIMAL CELLS, Pages 54-59, Sanetaka Shirahata, Kiichiro Teruya, Kiyohiko Seki, Tetsuya Mori, Hideya Ohashi, Hirofumi Tachibana, Hiroki Murakami
IMMUNOGLOBULIN GENE EXPRESSION DURING HYBRIDOMA BATCH AND SEMICONTINUOUS CULTURES, Pages 60-67, M. Leno, O.W. Merten, D. Moeurs, H. Keller, L. Cabanié, J. Hache
A NOVEL MAMMALIAN EXPRESSION SYSTEM BASED ON THE GLOBIN LOCUS CONTROL REGION, Pages 68-70, J McLean, M Hollis, M Needham, C Gooding, K Hudson, D Scanlon, F McLaughlin, F Grosveld, M Antoniou
TRANSIENT INCREASE OF PRODUCTION IN FUSED RECOMBINANT CHO CELLS, Pages 71-74, Lars Adamson, Johan Dixelius, Jack Spira
A Comparison of Eukaryotic Control Elements using Tissue Plasminogen Activator (tPA) as a Reporter Gene, Pages 75-77, A.W. Grierson, C. Darnbrough, C. MacDonald
DISSOCIATION OF MAB PRODUCTION FROM CELL DIVISION USING DNA BIOSYNTHESIS INHIBITORS, Pages 81-88, K. Modha, J.P. Whiteside, R.E. Spier
THE EFFECT OF TETRAMETHYL UREA ON α-INTERFERON PRODUCTION BY NAMALWA CELLS, Pages 89-91, S.C. Musgrave, N.J. Bangerter, C.M. Bentley, C.D. Brown, Y.E. Douglas, N.J. Furminger, S.T. McGowan, M.F. Scott
THE EFFECT OF SEQUENTIAL INDUCTION OF α-INTERFERON PRODUCTION BY NAMALWA CELLS, Pages 92-94, S.C. Musgrave, C.M. Bentley, C.D. Brown, G.G. Feeney, N.J. Furminger, T.W. Steward
THE INFLUENCE OF SOME NATURAL AND SYNTHETICAL SUBSTRATA UPON THE BEHAVIOUR OF SOME CELL LINES IN VITRO, Pages 95-97, Nicolae Mirancea, Dorina Mirancea
DIFFERENTIATED LIVER FUNCTION IN RAT HEPATOCYTES TRANSFECTED WITH SV40 VIRAL DNA, Pages 98-100, Caroline MacDonald, David Campbell, Andrew Nairn, Brian Willett, Maureen Vass, Alexander Scott, Helen Grant
DETERMINANT FACTORS FOR EXPRESSION OF RECOMBINANT GENES IN CHO CELLS, Pages 101-103, P. Newton, A. Thompson, R. Anwar, A.J. Dickson, K. Gull
New Kinetic Parameters of the Growth of Animal Cells, Pages 104-107, W. Renner, C. Leist, A. Fiechter, H.M. Eppenberger
THE PREPARATION FROM HUMAN PLASMA OF A CELL CULTURE MEDIUM SUPPLEMENT THAT CAN BE PASTEURISED, Pages 111-113, A.J. MacLeod, H Hart, S.J. Pelly, A.A. Thompson
FREE FATTY ACID UTILIZATION OF DIFFERENT HYBRIDOMA CELL LINES AND ITS EFFECT ON CELL GROWTH, METABOLISM AND ANTIBODY FORMATION, Pages 114-116, Georg Schmid, Francois Huber, Gottfried Oesterhelt
PLURONIC ADSORPTION EFFECTS IN CELL CULTURE MEDIA, Pages 117-121, A. Handa-Corrigan, P. Kwasowski, J.Y. Zhu, S. Zhang, L. Zhang, N. Duggal, T. Odeby, R. Dagwell
INFLUENCE OF MEDIUM COMPOSITION ON THE DEATH AND LYSIS OF HYBRIDOMA CELLS IN CONTINUOUS CULTURES, Pages 122-124, J.L. GOERGEN, A. MARC, J.M. ENGASSER
BASIC STRUCTURE OF A DATABANK - KNOWLEDGE-BASED SYSTEM FOR THE COMPUTER AIDED DESIGN OF SERUM-FREE CELL CULTURE MEDIA, Pages 127-129, Sergio Ramírez, Manfred Huelscher
LONG TERM SERUM-FREE HOLLOW-FIBRE CULTURE OF CELL LINES PRODUCING HUMAN MONOCLONAL ANTIBODIES: METABOLIC ASPECTS, Pages 130-133, J.M. Davis, J.A.J. Hanak, G.M. Lewis, R. Chung, J. Faulkner
SERUM FREE CRYOPRESERVATION OF ANTIBODY PRODUCING CELLS, Pages 134-136, J.A.J. Hanak, R. Chung, G. Lewis, J. Faulkner, J.M. Davis
GROWTH OF HeLa CELLS IN A CHEMICALLY DEFINED MEDI-CULT® MEDIUM, Pages 137-139, Allesøe Karen, H. Ingolf Nielsen, Anna-Lise Poulsen
ADAPTATION OF rCHO CELLS TO GROWTH IN SERUM FREE MEDIUM: A FLOW CYTOMETRIC STUDY, Pages 140-145, Nicole Borth, Christine Schmatz, Jutta Hofbaur, Manfred Reiter, Theo Gaida, Gerald Blüml, Hermann Katinger
GROWTH, SUBCULTURE AND CRYOPRESERVATION OF ANCHORAGE-DEPENDENT CELLS: A COMPLETE SERUM-FREE SYSTEM, Pages 146-148, L. Kaspi, D. Fiorentini, R. Talmon, G. Bennett
Cryoprotection and cultivation of hybridoma cell lines under serum free condition, Pages 149-151, William Werz, Jutta Maucher, Bettina Reutter, Rolf G. Werner, Wolfgang Berthold
PRODUCTION AND CHARACTERIZATION OF A RECOMBINANT FACTOR VIII EXPRESSED BY CHO CELLS CULTURED IN SERUM SUPPLEMENTED OR IN SERUM FREE MEDIA, Pages 152-154, G. MIGNOT, N. BIHOREAU, P. PAOLANTONACCI, A. SAUGER, V. TOULLY, J.L. ROMET-LEMONNE
A DEFINED SERUM-FREE MEDIUM FOR DIVERSE CELL CULTURE APPLICATIONS, Pages 155-157, A. Handa-Corrigan;, A. Garcia de Castro, M. Chadd, S. Zhang
ADAPTATION OF A MURINE MONOCLONAL HYBRIDOMA CELL LINE TO A LOW COST SERUMFREE MEDIUM BY A REPEATED BATCH FERMENTATION PROCEDURE, Pages 158-160, U. Marx, H. Tanzmann, V. Jaeger, R.v. Baehr
POTASSIUM IONS AFFECT THE AMMONIA/AMMONIUM ION TOXICITY IN ANIMAL CELL CULTIVATIONS, Pages 163-165, Kristina Martinelle, Lena Häggsröm
DEVELOPMENT OF STRATEGIES FOR THE REMOVAL OF AMMONIA FROM ANIMAL CELL CULTURES, Pages 166-170, D CARBONELL, B BESNAINOU, J CAPIAUMONT, C LEGRAND, P LESSART, P NABET
INTEGRATED DETOXIFICATION: REDUCTION OF AMMONIUM CONCENTRATION BY DIALYSIS WITH CATION EXCHANGE MEMBRANES, Pages 171-175, J. Thömmes, U. Garske, M. Biselli, C. Wandrey
GROWTH AND METABOLISM OF RECOMBINANT CHO CELL-LINES IN SERUM FREE MEDIUM CONTAINING DERIVATIVES OF GLUTAMINE, Pages 176-179, A-C. Holmlund, N. Chatzisavido, S.L. Bell, E. Lindner-Olsson
GENETIC MODIFICATION OF HYBRIDOMA GLUTAMINE METABOLISM: PHYSIOLOGICAL CONSEQUENCES, Pages 180-182, S.L. Bell, M.E. Bushell, M.F. Scott, J.N. Wardell, R.E. Spier, P.G. Sanders
GROWTH STUDY OF LACTATE AND AMMONIA DOUBLE-RESISTANT CLONES OF HL-60 CELLS, Pages 183-185, Bénédicte Schumpp, Ernst-Jürgen Schlaeger
THE EFFECT OF REDUCED TEMPERATURE ON α-INTERFERON PRODUCTION BY NAMALWA CELLS, Pages 189-191, S.C. Musgrave, N.J. Bangerter, C.M. Bentley, C.D. Brown, T.W. Steward
EFFECT OF REDUCING THE SERUM OR ALBUMIN CONCENTRATIONS ON THE SHEAR SENSITIVITY OF TWO HYBRIDOMA CELL LINES IN SPARGED CULTURES, Pages 192-194, Leo van der Pol, Johannes Tramper
MECHANICAL DAMAGE OF SUSPENDED ANIMAL CELLS IN DIRECTLY SPARGED BIOREACTORS, Pages 195-202, Manfred Huelscher, Ulfert Onken
ADAPTATION OF HYBRIDOMA CELLS TO HYDRODYNAMIC STRESS UNDER CONTINUOUS CULTURE CONDITIONS, Pages 203-205, Georg Schmid, Francois Huber, Randolf Kerschbaumer
CALCIUM TRANSIENTS IN SF-9 INSECT CELLS EXPOSED TO FLUID STRESSES REPRESENTATIVE OF TURBULENT EDDIES, Pages 206-211, Robert S. Cherry, Laura E. Aloi
DISSOLVED OXYGEN - THE CRITICAL PARAMETER AT CELL GROWTH AND EXPRESSION OF r-PROTEIN IN A POROUS MICROCARRIER SYSTEM, Pages 212-214, J. Almgren, K. Nilsson, L. Nilsson
THE BIOLOGICAL EFFECTS OF DISSOLVED OXYGEN TENSION ON A HYBRIDOMA CELL LINE AND ITS CONTROL AS A MEANS OF IMPROVING MAb PRODUCTION IN A PROTEIN-FREE MEDIUM, Pages 215-217, Ruey-Bin Sheu, Steven Reid, Paul Greenfield
THE EFFECT OF HIGH GAS SPARGE RATES IN AIRLIFT FERMENTER CULTURE ON HYBRIDOMA CELL GROWTH AND ANTIBODY PRODUCTION IN LOW PROTEIN MEDIUM, Pages 218-220, S.J. Fyfe, R.C. Boraston, C.M. Marshall, S.A.M. Gardiner
A KINETIC STUDY OF HYBRIDOMA SEMICONTINUOUS CULTURE: EFFECTS OF DILUTION RATE, Pages 221-223, M. Leno, O.W. Merten, D. Moeurs, J Hache
Preliminary 1H-NMR Studies of a Tumour Cell, Pages 227-229, P.J. Phillips, J.P. Barford, C. Harbour, C.P. Marquis
A SIMULATION OF HYBRIDOMA GROWTH INCLUDING SUGAR AND AMINO ACIDS PROFILES, Pages 230-232, John Barford, Peter Phillips, Edward Varga, Colin Harbour
Carbohydrate metabolism, Pages 233-242, C.P. Marquis, C. Harbour, J.P. Barford, P. Phillips
ENERGY METABOLISM OF MURINE HYBRIDOMAS, Pages 243-247, H.A. Jenkins, L. Fitzpatrick, M. Butler
COMPLEX MEDIUM SUPPLEMENTS GIVE DIFFICULTIES WHEN INVESTIGATING MAMMALIAN CELL PHYSIOLOGY, Pages 248-250, Henrik Albahn Hansen, Claus Emborg
METABOLIC QUOTIENTS FOR RECOMBINANT BHK CELLS GROWN IN REPEATED BATCH MODE ON CYTODEX III MICROCARRIERS, Pages 251-253, G. Schmid, H. Zilg, R. Johannsen
HYBRIDOMA GROWTH AND ANTIBODY PRODUCTION IN EXTENDED STATIONARY STATES, Pages 254-258, B. Amos, M.Al. Rubeai, A.N. Emery
METABOLIC MANAGEMENT OF A HYBRIDOMA CELL LINE, Pages 261-263, Kevin Murray, Alan J Dickson, Keith Gull
LONG TERM APPLICATION OF MEDIUM RECYCLING FOR ECONOMIC ANTIBODY PRODUCTION, Pages 264-268, Ralph Kempken, Heino Büntemeyer, Jürgen Lehmann
CULTIVATION STRATEGY AND BIOREACTOR SYSTEM FOR OPTIMAL MEDIUM RE-USE AND DETOXIFICATION DURING CONTINUOUS PERFUSION PROCESSES, Pages 269-275, H. Büntemeyer, J. Lehmann
APPLICABILITY OF FED BATCH PROCESSES FOR IMPROVING RECOMBINANT PROTEIN PRODUCTION BY THE BACULOVIRUS EXPRESSION SYSTEM, Pages 276-281, S. Reid, V. Saxena, P.F. Greenfield, S.A. Weiss
AMINO ACID METABOLISM IN BATCH AND FED-BATCH CULTURE OF A MURINE MYELOMA CELL, Pages 282-284, Jan Ljunggren, Lena Häggström
CONTINUOUS HYBRIDOMA SUSPENSION CULTURES: CHANGES IN FEED MEDIUM COMPOSITION AND THEIR EFFECTS ON EXTRACELLULAR AND INTRACELLULAR METABOLITE CONCENTRATIONS, Pages 285-291, Georg Schmid, Thoralf Keller
EXPERIMENTAL VALIDATION OF A REACTION MECHANISM FOR A CLASS OF ANIMAL CELL CULTURE, Pages 292-297, Véronique Chotteau, Georges Bastin, John Wérenne, Luc Fabry
MONITORING OF BIOSYNTHETIC AND METABOLIC ACTIVITY IN ANIMAL CELL CULTURE USING FLOW CYTOMETRIC METHODS, Pages 301-307, M. Al-Rubeai, M. Kloppinger, G. Fertig, A.N. Emery, H.G. Miltenburger
MONITORING AND CONTROL OF HYBRIDOMA CELL LINE MN12 IN A CONTINUOUS PERFUSION CULTURE SYSTEM, Pages 308-311, J.M.Coco Martin, C.A.M. van der Velden-de Groot, R.C. Dorresteijn, F. Brunink, E.C. Beuvery
DEVELOPMENT OF A NOVEL MICROMANIPULATION TECHNIQUE FOR DETERMINING MECHANICAL PROPERTIES OF SINGLE MAMMALIAN CELLS, Pages 312-318, Zhibing Zhang, Mike Ferenczi, Colin Thomas
RELEASE OF CELLULAR ENZYMES FOR EVALUATING THE DEAD CELL NUMBER IN BIOREACTOR CULTURES, Pages 319-324, O-W MERTEN, H KELLER, L CABANIE, C van KAN MARTIN, D MOEURS
QUANTITATIVE CELL ADHESION MEASUREMENTS ON SUBSTRATES OF VARYING WETTABILITY, Pages 325-327, Richard Brydges, Nicholas Maroudas, Anita Handa-Corrigan
PRODUCTIVITY OF A HYBRIDOMA CELL LINE IN A RANGE OF SUSPENSION AND IMMOBILISED CULTURE SYSTEMS, Pages 331-335, D. Looby, J.B. Griffiths, A.J. Racher
HUMAN HYBRIDOMA PRODUCING ANTIBODIES TO PSEUDOMONAS AERUGINOSA: SCALE UP AND OPTIMIZATION OF IgM PRODUCTION IN STIRRED BIOREACTORS, Pages 336-341, U. SCHüRCH, S.J. CRYZ Jr., A.B. LANG
COMPARISON OF GROWTH AND ANTIBODY PRODUCTION OF A HYBRIDOMA CELL LINE GROWN IN STATIC FLASK AND SUSPENSION CULTURE, Pages 342-344, W.A. Southwick, C.J. Mannix
PERFUSION VERSUS BATCH CULTURE: WHICH IS THE PREFERRED PROCESS?, Pages 347-350, Peter W. Runstadler
PERFUSION SYSTEMS FOR HYBRIDOMA CELLS BASED ON SEDIMENTATION IN CHAMBERS AND ERLENMEYER FLASKS, Pages 351-353, Karen Mette, Krieger Lassen, Claus Emborg
ASPECTS OF CONTINUOUS CULTURE VERSUS BATCH MODE FOR ANIMAL CELLS IN INDUSTRIAL PROCESSES, Pages 354-364, Wolfgang Noé, Rolf Bux, Rolf G. Werner, Wolfgang Berthold, Karl Thomae GmbH, Biberach an der Riss
PERFUSION BIOREACTOR PERFORMANCE AT DIFFERENT CELL BLEED RATES, Pages 365-372, C. Fenge, E. Fraune, K. Schügerl
A STUDY OF AN INEXPENSIVE PERFUSION OF A MOUSE HYBRIDOMA, Pages 373-375, C.P. Marquis, C. Harbour, J.P. Barford, P. Phillips
A COMPARATIVE STUDY OF MONOCLONAL ANTIBODY YIELD USING BATCH, CONTINUOUS OR PERFUSION SUSPENSION CULTURE TECHNIQUES, Pages 376-378, A. Bartley, A.J. MacLeod
DEVELOPMENT OF A REACTOR-INTEGRATED AERATION SYSTEM FOR CULTIVATION OF ANIMAL CELLS IN FLUIDIZED BEDS, Pages 381-385, Berthold Hambach, Manfred Biselli, Peter W. Runstadler, Christian Wandrey
OXYGENATION IN FLUIDIZED BED BIOREACTORS USING THE MICROSPARGING TECHNIQUE, Pages 386-392, M. Reiter, N. Zach, T. Gaida, G. Blüml, O. Doblhoff-Dier, F. Unterluggauer, H. Katinger
PILOT-SCALE MODULAR HARD-AND SOFTWARE CONCEPT FOR ANIMAL CELL FERMENTATION, Pages 393-396, O. Doblhoff-Dier, F. Unterluggauer, S. Huss, R. Plail, H.W.D. Katinger
A NOVEL PERFUSION SYSTEM FOR THE LARGE-SCALE CULTIVATION OF ANIMAL CELLS BASED ON A CONTINUOUS FLOW CENTRIFUGE, Pages 397-402, V. Jäger
PRODUCTION TISSUE CULTURE BY ROBOTS, Pages 403-408, Richard Archer, Lesley Wood
AGGREGATE SUSPENSION CULTURES OF BHK CELLS, Pages 411-413, J.L. Moreira, P.M. Alves, J.G. Aunins, M.J.T. Carrondo
THE GROWTH OF VERO CELLS AS SUSPENDED AGGREGATES IN SERUM-FREE MEDIUM, Pages 414-417, Jack Litwin
Tuning of shear sensitivity of CHO cells and its correlation with the size distribution of cell aggregates, Pages 418-420, M. Jordan, W. Renner, H. Sucker, C. Leist, H.M. Eppenberger
HIGH DENSITY AGGREGATE CULTURE OF RECOMBINANT CHO CELLS IN FLUIDIZED BED BIOREACTORS, Pages 421-423, M. Reiter, G. Blüml, T. Gaida, N. Zach, C. Schmatz, N. Borth, O. Hohenwarter, H. Katinger
ELIMINATION OF CELL AGGREGATION IN SUSPENSION CULTURES OF CHINESE HAMSTER OVARY (CHO) CELLS, Pages 424-426, R. Boraston, C. Marshall, P. Norman, G. Renner, J. Warner
EVALUATION OF A SPIN FILTER DURING PERFUSION CULTURE OF RECOMBINANT CHO CELLS, Pages 429-433, C Fenge, F Buzsaky, E Fraune, E Lindner-Olsson
SPIN FILTER FOR CONTINUOUS PERFUSION OF SUSPENSION CELLS, Pages 434-436, U. Siegel, C. Fenge, E. Fraune
Improved cell retention system based on the rotating sieve technique, Pages 437-447, Zoltán Sümeghy
OPTIMIZATION OF SPIN-FILTER PERFORMANCE IN THE INTENSIVE CULTURE OF SUSPENDED CELLS, Pages 448-451, D.C.H. Jan, A.N. Emery, M. Al-Rubeai
METABOLITE PARAMETERS MODULATING MAB PRODUCTION IN A FIXED BED, GLASS SPHERE BIOREACTOR, Pages 455-459, A.J. Racher, D. Looby, J.B. Griffiths
CONTINUOUS PRODUCTION OF MONOCLONAL ANTIBODIES IN CELLIGEN PACKED BED REACTOR USING FIBRA-CEL CARRIER, Pages 460-464, G. Wang, W. Zhang, D. Freedman, L. Eppstein, A Kadouri
Collagenated vesicular plant parenchym material as a new type of surface-carrier in animal cell culture, Pages 465-468, B. Röder, H. Kutsch
IMMOBILIZATION OF ANIMAL CELLS ON CHEMICALLY MODIFIED SIRAN CARRIER, Pages 469-475, Elke Lüllau, Claus Dreisbach, Alain Grogg, Manfred Biselli, Christian Wandrey
Introduction to the session on fluidised bed cultures by Schierer, Page 479
HIGH DENSITY BHK CULTURE USING POROUS MICROCARRIERS, Pages 480-487, D.W. Lee, D. Gregory, J.M. Piret, D.G. Kilburn, D.J. Haddow
THE PRODUCTION OF RECOMBINANT HUMAN INTERLEUKIN-2 IN A FLUIDIZED BED BIOREACTOR, Pages 488-494, Ricardo Kratje, Roland Wagner
FLUIDIZED-BED BIOREACTOR PERFUSION CULTURE FOR THE PRODUCTION OF THERAPEUTIC PROTEINS, Pages 495-500, Peter W. Runstadler, Amar S. Tung, Nitya G. Ray
DEVELOPMENT OF A NEW TYPE OF MACROPOROUS CARRIER, Pages 501-504, G. Blüml, M. Reiter, N. Zach, T. Gaida, C. Schmatz, K. Strutzenberger, T. Mohr, B. Rauschert, H. Katinger
SCALE-UP OF A FLUIDIZED BED REACTOR OPERATED WITH POROUS GLASS CARRIERS, Pages 505-507, F. Unterluggauer, O. Doblhoff, M. Reiter, G. Blüml, N. Zach, G. Kral, Ch. Schmatz, H. Katinger
EFFICIENT SERIAL PROPAGATION OF WI38 CELLS ON POROUS MICROCARRIERS (CULTISPHER GL), Pages 508-510, G.R. Pettman, C.J. Mannix
PRODUCTION OF T-PA BY C-127 CELLS CULTIVATED ON DIFFERENT POROUS MICROCARRIERS IN A PERFUSED FLUIDIZED BED REACTOR, Pages 511-513, Peter Reuschenbach
SCALING-UP OF A HOLLOW FIBRE REACTOR FOR ANIMAL CELL CULTIVATION, Pages 517-523, I. Jöbses, P. van Zutphen, J. Oomens, A van Os, O. Schönherr
PRODUCTION OF CONCENTRATED MONOCLONAL ANTIBODY CONTAINING SUPERNATANT IN MICROPOROUS CERAMIC TUBES, Pages 524-526, øystein W. Rønning, Anne-Karine W. Haflan, Mona Schartum
SCALE-UP OF HUMAN IgG1 PRODUCTION BY A LYMPHOBLASTOID CELL LINE IN HOLLOW FIBER SYSTEMS, Pages 527-529, F. DHAINAUT, J.L. METERREAU, M.P. MAS, C. POTENTINI, G. MIGNOT
Long Term Production of Soluble CD23 in Hollow Fiber Reactors, Pages 530-533, C. Isch, A. Bernard, J.Y. Bonnefoy, C. Cavegn, P. Graber, T. Jamotte, H.D. Blasey
SIMULTANEOUS CULTIVATION OF TWO HYBRIDOMA CELL LINES IN A HOLLOW FIBER BIOREACTOR, Pages 534-537, U. Marx, H. Tanzmann, V. Jaeger, S.T. Kiessig, R.v. Baehr
A DUAL FIBRE MEMBRANE REACTOR FOR THE CULTIVATION OF MAMMALIAN CELLS, Pages 538-540, R.B. COUSINS, R. GERGEN, F.J. GERNER
PRODUCTION OF COLLAGENASE BY ANCHORAGE DEPENDENT CELLS IN CULTURE, Pages 543-546, T. Marique, D. Blankaert, J. Wérenne
ACTIVATION OF A SPECIFIC PROTEOLYTIC ACTIVITY IN SUSPENSION CULTURES OF RECOMBINANT ADHERENT CELLS, Pages 547-553, Shunjiro Sugimoto, Waldemar Lind, Roland Wagner
DEPENDENCE OF THE PHARMACOKINETIC PROPERTIES OF MONOCLONAL IgM ON ITS N-GYCOSYLAYTION, Pages 557-561, R PAREKH
PRODUCTION OF RECOMBINANT SOLUBLE HUMAN TNF RECEPTOR USING THE BACULOVIRUS-INSECT CELL EXPRESSION SYSTEM, Pages 562-568, E.-J. Schlaeger, H. Loetscher, R. Gentz
EXPRESSION OF RECOMBINANT PROTEINS IN HIGH DENSITY INSECT CELL CULTURES, Pages 569-578, C. CAVEGN, H.D. BLASEY, M.A. PAYTON, B. ALLET, J. LI, A.R. BERNARD
PRODUCTION OF RECOMBINANT EPIDERMAL GROWTH FACTOR RECEPTOR TYROSINE KINASE (EGFR-TK) DOMAIN IN AN INSECT CELL/BACULOVIRUS EXPRESSION SYSTEM, Pages 579-582, B.S. Baines, B. Baker, M. Gregoriou, A.M. Hutchinson, J.E. Horne, K. Khan, Y. Obisesan, G. Shah
Recombinant Protein Production by a Murine Plasmacytoma, Pages 583-585, C.A. Mitchell, P.P. Gray
Methods useful for the generation of bispecific antibodies, Pages 586-592, E Stanger, W Scheirer, E Pursch, P Peichl
PRODUCTION OF HUMAN GAMMA-GLUTAMYLTRANSFERASE BY MASS CULTURES OF HUMAN HEPATOMA AND RECOMBINANT CHO CELLS, Pages 593-595, J.L. GOERGEN, I. CHEVALOT, A. VISVIKIS, T. OSTER, K. HESS, J.M. ENGASSER, A. MARC
PRODUCTION OF RECOMBINANT RUBELLA ANTIGENS, Pages 596-598, S. Al-Mumin, J. Newcombe, W. Starkey, J.M. Best, R.E. Spier, P.G. Sanders
NOVEL PLASMINOGEN ACTIVATORS FROM THE VAMPIRE BAT DESMODUS ROTUNDUS, Pages 599-604, T. Petri, B. Baldus, W. Boidol, P. Bringmann, L. Cashion, P. Donner, B. Haendler, J. Kraetzschmar, G. Langer, G. Siewert, W. Witt, W.-D. Schleuning
IMMUNOGLOBULIN FRAGMENTS DURING FERMENTATION OF A HUMAN MONOCLONAL ANTI-ERYTHROCYTE ANTIBODY, Pages 605-609, S.T. Kießig, U. Marx, B. Wilke, G. Hausdorf
Microporous Membrane Ion Exchanger for Rapid Purification of Monoclonal Antibodies, Pages 613-620, D. Lütkemeyer, S. Siwiora, H. Büntemeyer, J. Lehmann
PURIFICATION OF MONOCLONAL ANTIBODY FROM CULTURE SUPERNATANT PRODUCED IN HOLLOW FIBRE BIOREACTORS, Pages 621-623, Margaret Goodall, Tracy L Bentley, Royston Jefferis
Virus Removal/Inactivation in Downstream Processing, Pages 624-634, J. Walter, W. Werz, P. McGoff, R.-G. Werner, W. Berthold
A UNIQUE AND VALIDATABLE MEMBRANE-BASED SYSTEM CAPABLE OF HIGH RESOLUTION REMOVAL OF VIRUSES FROM PROTEIN SOLUTIONS, Pages 635-638, Paul Sekhri, Anthony DiLeo, Anthony Allegrezza, Richard Levy
RETENTION OF VIRAL CONTAMINANTS BY MICROFILTRATION, Pages 639-641, Peter Ball, Alastair Hunter, Tony Orchard
RETROVIRUSES: REGULATORY ISSUES FOR BIOLOGICAL PRODUCTS AND VIRUS VECTORS, Pages 645-652, D Onions, G Lees
DETECTION OF VIRUSES IN RECOMBINANT CELLS BY ELECTRON MICROSCOPY, Pages 653-657, Catherine Liptrot, Keith Gull
The Detection of Human Viruses in Cell Lines, Pages 658-660, M Kelly, K Blake, A Doyle
NEW CELL LINES FOR VIRUS DIAGNOSIS, Pages 661-663, J. Clarke, C. MacDonald, K. Blake, J.B. Griffiths
THE DEVELOPMENT OF APPROPRIATE VIRUS MODELS FOR VALIDATING THERAPEUTIC PROTEIN PURIFICATION PROCESSES, Pages 664-667, C. Harbour, J. Spencer, G. Woodhouse, J.P. Barford
PRODUCT PROCESS VALIDATION WITH HUMAN IMMUNODEFICIENCY VIRUS-I, Pages 668-670, R.J. Smith, K. Blake, A. Doyle
Validation of Processes for the Removal and/or Inactivation of These Agents, Pages 671-677, A.J. Darling, K.T. Smith, J. Manson, J. Hope
VALIDATION OF DNA FINGERPRINTING IN ANIMAL CELL TECHNOLOGY: THE DIFFERENTIATION AND IDENTIFICATION OF MURINE HYBRIDOMA CLONES FOR A SINGLE FUSION AND THE STABILITY OF TRANSFORMED CELL LINES, Pages 681-688, G.N. Stacey, B.J. Bolton, D. Morgan, A. Doyle
MOLECULAR HYBRIDIZATION TECHNIQUES AND POLYMERASE CHAIN REACTION (PCR) AS METHODS FOR SAFETY ASSESSMENT OF ANIMAL CELLS USED IN BIOPHARMACEUTICAL PRODUCTION, Pages 689-695, Jeffrey M. Ostrove, William Walsh, Dominick Vacante, Nita Patel
QUANTITATION OF RESIDUAL DNA IN BIOLOGICAL PRODUCTS: NEW REGULATORY CONCERNS AND NEW METHODOLOGIES, Pages 696-698, Kenneth T. Smith, lain Doherty, Julie A. Thomas, Steven R. Per, Alex F. Sito
THE DEVELOPMENT AND VALIDATION OF IN VITRO TOXICITY TESTS, Pages 701-712, Michael Balls
RENAL EPITHELIAL CELLS IN CULTURE: A COMPARATIVE STUDY OF THE FUNCTIONAL ACTIVITIES OF RABBIT KIDNEY PROXIMAL TUBULE CELLS IN PRIMARY CULTURES AND THE LLC-PK1 AND OK CELLS, Pages 713-715, J P MORIN, C MONTEIL, J P FILLASTRE
RABBIT KIDNEY PROXIMAL TUBULE CELLS IN PRIMARY CULTURE AS AN IN-VITRO MODEL FOR EVALUATING PLATINUM-DERIVATE-INDUCED NEPHROTOXICITY, Pages 716-721, Fraçoise Courjault, Danielle Leroy, André Cordier, Hervé Toutain
SERTOLI CELL CULTURE MODEL FOR ASSESSING TESTICULAR TOXICITY, Pages 722-727, P.H. BRUN, Ph. PICAUT, J.F. LEONARD, A. CORDIER
CAN A CYTOTOXICITY TEST BE DISCRIMINANT FOR TARGET ORGAN TOXICITY ?, Pages 728-730, P. VINTEZOU, P.H. BRUN, I. DIARTE, J.F. LEONARD, C. CHONG, C. MELCION, A. CORDIER
11th ESACT MEETING - UNIVERSITY OF SUSSEX 1991 LIST OF DELEGATES, Pages 731-740
Subject index, Pages 741-751
Author index, Pages 753-756