Animal Cell Technology Meets Genomics: Proceedings of the 18th ESACT Meeting Granada, Spain, May 11–14, 2003

Effect of Osmotic Pressure on Gs-Ns0 Expression System....Pages 3-13
Molecular Approaches to Influence Epigenetic Effectors of Transient and Stable Transgene Expression in Mammalian Cells....Pages 15-22
Genomic Exploration on Chinese Hamster Ovary Cells....Pages 23-29
Roles for Glycosylation in Receptor-Ligand Interactions in the Immune System....Pages 31-42
Investigations on Mannose-6-Phosphate Receptor Mediated Protein Uptake....Pages 43-49
Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System....Pages 51-57
Inhibiting Apoptosis in Cell Culture Using Multiple Inhibitors....Pages 59-65
Pausing of Mammalian Cells by Cold Exposure, Limits and Opportunities....Pages 67-70
The Functional Competence of Animal Cells: Anal Ysis of the Secretory Pathway....Pages 71-74
Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress....Pages 75-78
Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medium....Pages 79-82
Intracellular Nucleotide Pools for Optimizing Product-Oriented Transient Transfection of Hek293 Cells in Suspension....Pages 83-86
Impact of Yeast Pyruvate Carboxylase on the Productivity of Animal Host Cell Lines....Pages 87-89
Environmental Effects of Lactate on High-Five™ Insect Cell Metabolism....Pages 91-94
Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the Application of Multivariate Methods....Pages 95-98
Engineering Trichoplusia ni (High-Five™) Insect Cells to Express a Cytosolic Pyruvate Carboxylase Enzyme Improves the Viability of the Cells in Batch, Fed-Batch and Perfusion Cultures....Pages 99-102
Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations....Pages 103-106
Antiapoptotic Activity of Synthetic and Nnatural Peptides....Pages 107-110
Effect of Antiapoptotic Genes Expression on Cell Growth and Monoclonal Antibody Productivity in a Hybridoma Cell Line....Pages 111-113
Amplified Dicistronic Expression Units Mediate Apoptosis Protection in CHO-DG44 Cells Adapted for Growth in Serum-Free Media, Impact on Mitochondria Copy Number....Pages 115-120
Effect of Reduced Water on the Apoptotic Cell Death Triggered by Oxidative Stress in Pancreatic β HIT-T15 Cell....Pages 121-124
Characterization of Apoptosis in a CHO Cell Line Cultivated in Batch and Continuous Culture: Effect of Medium, Specific Perfusion Rate and Chemical Inhibitors....Pages 125-128
Hydrogen Peroxide-Induced Cellular Senescence is Regulated via Two Different Pathways....Pages 129-131
Identification of Autocrine Factors Influencing Proliferation in Serum-Free Cultures of Trichoplusia NI Cells....Pages 133-135
The Effects of Insulin and Long™R 3 IGF-I on Chinese Hamster Ovary Cells: Cell Survival, Receptor Activation and Second Messenger Pathways....Pages 137-141
Conditioned Medium Factors in Protein-Free Cultures of NS0 Cells....Pages 143-145
Production of Human Anti-Peptide Antibody for Clinical Use by In Vitro Immunization....Pages 147-149
Optimization of in Vitro Immunization Protocol to Produce Antigen Specific Human Monoclonal Antibody by Demonstrating the Role of IL-10....Pages 151-153
Eextracellular Histone H4 from SF9 Cells Is Antimicrobial....Pages 155-157
Analyzing Calcium Phosphate Transfection of Adherent CHO Cells Using Microscopic Live Imaging....Pages 159-161
Effects of Multiplicity of Infection (MOI) and Cell Cycle on Baculovirus Infection Kinetics....Pages 163-165
Non-Freezing Preservation and Subsequent Recovery of Hepatocytes Under PO 2 - and pH-Controlled Conditions for Continuous Supply of Cells for Bioartificial Liver Support....Pages 167-170
Selection of MDCK Subclones with Various Genotypic and Phenotypic Markers: Sensitivity to Influenza Virus Infection....Pages 171-174
Extracellular Metalloproteases and Their Inhibitors Expressed by The IFN-γ Producing CHO-320 Cell Line....Pages 175-177
Novel Small-Molecule AKT1 Inhibitors Discovered by Redistribution™-Based High- Throughput Screening....Pages 179-181
Influence of Mistletoe Extracts and Its Components on in Vitro Physiology of Cancer Cells....Pages 183-185
Rapid in-Process Monitoring of Antibody Integrity, Purity and Titre- 2100 Bioanalyser (Lab on a Chip) Study....Pages 187-192
Toxicological Evaluation of Haine River Waste-Water Samples to Appropriately Sensitized Cultured Fathead Minnow Cells Compared With the Microtox® Method....Pages 193-196
3D-Cultivation and Characterisation of Osteogenic Cells for the Production of Highly Viable Bone Tissue Implants....Pages 199-205
Carbon Source Has a Considerable Influence on Extracellular Matrix Formation, Demonstrated on Specific Collagen Expression of Primary Human Tenocytes....Pages 207-212
Design and Characterization of Cardiomyocyte-Derived Microtissues....Pages 213-219
Process Development for Standardized Generation of Monocyte-Derived Dendritic Cells: Applicability in Breast Cancer Immunotherapy....Pages 221-227
Stemline™ Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34 + Hematopoietic Stem Cells and Progenitors....Pages 229-232
Mesenchymal Stem Cells: A Very First Step Towards Large Scale Cell Culture Strategies....Pages 233-236
Expansion of Murine Embryonic Stem Cells on Microcarriers-Functional Characteristics and Scale-Up Potential....Pages 237-240
In Vitro Cultivation of Rabbit Mesenchymal Stromal Cells on 3D Bioresorbable Calcium Phosphate Scaffolds for the Generation of Bone Tissue Implants....Pages 241-243
Establishment and Characterization of Human Medullary Thyroid Carcinoma Cell Lines for Immunotherapy....Pages 245-248
Suppression of Cell Growth by Platinum Nanocolloids as Scavengers against Reactive Oxygen Species....Pages 249-251
Comparison of Fluidized Bed and Fed Batch Reactor Cultures for Production of Anti-HIV-Antibody....Pages 253-255
Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water....Pages 257-260
Spheroid Formation by Encapsulation of Cancer Cells to Mimic Small Size Tumors....Pages 261-263
Bioreactor for Continuous Biomechanical Characterization of Cellular Systems and Tissue-Engineered Biohybrid Tissues....Pages 265-267
Membrane-Separated Cocultivation of Cord Blood Hematopoietic Stem Cells with Stromal Cell Lines....Pages 269-271
Anti-Apoptotic Genes Protect an Insulinoma Cell Line From Cell Death Induced by Hypergl Ycemia....Pages 273-275
Preparation and Characterization of an Idiotype-Dendritic Cell Vaccing for Immunotherapy of Multiple Myeloma....Pages 277-280
Pretreatment with Polycations Enhances Adenoviral Infection Levels in Serum-Free PER.C6⊆ Cells as Well as Hek 293 Cells....Pages 281-283
Optimizing the Production of Cardiomyocytes from Mouse Embryonic Stem Cells in a 2L Stirred Tank Reactor....Pages 285-288
Establishment and Characterization of CD4+ Killer-T-Cells (KTCS) a Promising New Tool for Adoptive Immunotherapy....Pages 289-292
Successful Development of a Robust Adenovirus Production Process Primarily Relies on a Better Understanding of Packaging Cell Line Physiology and Vector Replication Kinetics....Pages 295-301
Retroviral Vector Stability: Inactivation Kinetics and Membrane Properties....Pages 303-308
Comparison of Host Cell Lines and Production Methods for a New Generation of Oncolytic Adenoviral Vectors....Pages 309-316
Process Development for a Veterinary Vaccine against Heartwater....Pages 317-320
A New Method for the Quantitative Determination of Enveloped Viral Particles....Pages 321-323
Development of a 293T Cell Line for the Production of Virus Like Particles in Bioreactors....Pages 325-328
Effect of MOI and Medium Composition on Adenovirus Infection Kinetics....Pages 329-332
Ion-Exchange Chromatography Based Strategies for Purification of Viral Vectors....Pages 333-338
Scaling up of Endothelial Cells Culture for the Production of a Rickettsiae Vaccine: Valuation of the Importance of the Interference Effects....Pages 339-341
Inducible Gene Expression for Antibiotic Drug Discovery and Diagnostics....Pages 345-350
Nucleic Acid Capture Assay: A Novel High- Throuhgput Method for Direct Mrna Quantitation....Pages 351-357
High Throughput Transient Transfections of Hek293Ebna Cells — Devel Opment of an Integrated Expression Purification Process at 100 ml Scale....Pages 359-364
Transient Gene Expression in Suspension Hek293 Cells: Application to Large-Scale Protein Production....Pages 365-367
Monoclonal Antibody Production: Determination of Appropriate System and Medium for Production Enhancement and Decrease of Overall Production Time....Pages 369-371
The Secrets of Transfection in Serumfree Suspension Culture....Pages 373-376
From Genes to Proteins: Full Open Reading Frame (ORF) Clones and Their Application in Functional Research....Pages 377-379
A Versatile Disposable Culture System for High Throughput Screening of Process Parameters and Production Cell Lines....Pages 381-383
Long Time Storage of Pre-Inoculated Multi-Well PLlates for Cell-Based Assay Kits Used for Drug Iedntification and Characterisation....Pages 385-387
Comparative Secretion Levels of Recombinant Proteins between Insect Cell-Bbaculovirus and Mammalian Hek293EBNA Cells....Pages 389-391
Microfluidic Technology Applied to Protein Sizing and Quantitation....Pages 393-396
Cryostock a Software for Cell Culture Management....Pages 397-400
Targeting the Human Immunoglobulin Loci for High Level Hgene....Pages 403-409
Mar Elements as Tools to Increase Protein Production by CHO Cells....Pages 411-415
Cell Line Engineering for Production of Therapeutic Antibody Glycoforms with Increased Biological Activity....Pages 417-422
Development of a Metabolically Optimized Fermentation Process Based on Glucose-Limited CHO Perfusion Culture....Pages 423-430
Applications of Quasi Real-Time Metabolic Flux Analysis in Mammalian Cell Culture Process Development....Pages 431-438
Data-Based Modelling of Cell Cultures....Pages 439-445
Cell Culture and Downstream Processing Integration Using Proteinchip® Technology Application to Protein Analysis and Purification in Biopharmaceutical Manufacturing....Pages 447-452
The Prospects for Insect Cells for Use as a Cell Substrate for the Production of Biotherapeutics....Pages 453-458
High Performance Cell Culture Platform Technology for MAb Production....Pages 459-464
Development and Integration of a New Animal-Component-Free Process for the Production of UK-279,276....Pages 465-470
Towards an Affinity-Based Capture System for the Isolation of High Expression Cells Using a Co-Expressed Surface Protein....Pages 471-473
Effect of Doxycycline-Regulated Calnexin and Calreticulin Expression on Specific Thrombopoietin Productivity of Recombinant CHO Cells....Pages 475-478
Recombinant Glycoprotein Expression Development of a Homologous CHO Expression System....Pages 479-482
Generation of Recombinant Antibody Manufacturing Cell Lines Using Cell-Cell Fusion....Pages 483-488
Cloning and Expression of Biopharmaceuticals in CHO.K1 and BHK-21 Cells....Pages 489-492
Camel Single-Domain Antibodies as Modular Building Blocks to Make Bivalent Constructs for Use in Immunotherapy of Cancer....Pages 493-495
Establishment of Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody....Pages 497-499
From Gene to Monoclonal Antibody: Efficient Screening by Cell Sorting....Pages 501-504
Development of an Integrated Strategy for Recombinant Cell Line Selection....Pages 505-507
Gene Transfer to a Animal Cells in Culture by Microinjection: A Novel Tool to Create Recombinant Cell Lines?....Pages 509-512
Evaluation of the Stability of NS0 Cell Lines Expressing Recombinant Human IgG....Pages 513-515
High Throughput Screening and Selection of Single Cells in Suspension Using Fluorescence Parameters....Pages 517-520
Control of Key Parameters in the Development of Mammalian Production Clones....Pages 521-524
Establishment of Screening Systems for Recombinant Cell Lines: Early Prediction of the Suitability as a Production Clone....Pages 525-527
Characterisation of CHO Subclones Showing Profound Changes in Performance When Propagated in Different Cultivation Systems....Pages 529-532
High Level Production of Recombinant IgG in the Human Cell Line PER.C6™....Pages 533-536
Creating a New Medium to Help Meet the Variable Nutritional Requirements of Chinese Hamster Ovary (CHO) Cell Clones....Pages 537-540
Development of an Efficient Medium Optimization Kit for Factorial Matrix Design—A Statistical Approach to Increase Cell Growth and Productivity of Recombinany CHO Cells....Pages 541-547
An Efficient Approach to Cell Culture Medium Optimization — A Statistical Method to Medium Mixing....Pages 549-553
Virus Inactivation in Foetal Calf Serum by a Combined Treatment of Gammairradiation and UV-C Irradiation....Pages 555-559
Advances in Media Optimization: Two Automated Approaches that Increase Expression While Reducing Development Time....Pages 561-563
The Importance of Cholesterol for Insect Cell Growth and Baculovirus Production....Pages 565-568
Use of Rapeseed Proteins and Peptides as Supplements in Insect Cell Cultures....Pages 569-571
Nutritional Performance of Wheat-Gluten-Derived Protein Hydrolysates in Hybridoma Cell Cultures. A Nutritional Function Study....Pages 573-575
Synthechol™ Ynthetic Cholesterol for Cholesterol Dependent Cell Culture — Development of Non-Animal Derived Chemically Defined NS0 Medium....Pages 577-580
NS0 Derivatives: MAB Production in Largescale SFM Formats....Pages 581-584
Silk Protein Sericin Accelerates Proliferation of Various Mammalian Cells....Pages 585-587
Image Analysis Based Real Time-Control of Glucose Concentration....Pages 589-592
Online Analysis of Microcarrier Cultivations....Pages 593-595
Error Analysis during Estimation of Metabolic Fluxes through Metabolite Balancing....Pages 597-600
Generalized Logistic Equation Modeling of Mammalian Cell Batch Cultures....Pages 601-604
In-Situ Microscopy Based Monitoring of Mammalian Cell Culture Processes....Pages 605-608
A Noel Disposable Microtube for Rapid Assessment of Biomass in Cell Cultures....Pages 609-612
Intracellular Monitoring of Baculovirus Infection of SF9 Insect Cells and R-Protein Production Using Anti-Alpha-1,3/4 Fucosyl Transferase Antibodies and Flow Cytometry....Pages 613-616
Effect of Glucose and Glutamine Concentration on Metabolism of Animal Cells in Chemostat Culture....Pages 617-620
Evaluation of a Novel apacitance Probe for On-Line Monitoring of Viable Cell Densities in Batch and Fed-Batch Animal Cell Culture Processes....Pages 621-624
Sodium Butyrate Enhancement of Protein Expression in Hela Cells: Follow-Up of a Chimeric Reporter Gene Behaviour for Process Development....Pages 625-627
Scalable, Serum-Free, High Efficiency Transfection of CHO Cells Using an Economical DNA Transfer Vehicle....Pages 629-632
Process Optimization for an NS0-Derived Hybridoma Cell Line in a Chemically Defined, Protein-Free Medium....Pages 633-636
Impact of Change in Fermentation Process pH and Dissolved Carbon Dioxide Concentration on Secreted Antibody Structure and Cell Physiology of a GS-NS0 Cell Line Expressing a Human Antibody....Pages 637-640
Experimental Factorial Design for the Study of Some Variables in Roller Bottles Cell Culture....Pages 641-643
Evaluation of Rollercell System for High Scale Production of Biopharmaceuticals....Pages 645-647
Strategies for the Process Development of Monoclonal Antonpdoes for Therapeutic Use....Pages 649-653
Optimisation of PEI-Mediated Transient Expression in Chinese Hamster Ovary Cells....Pages 655-657
Development of Fed-Batch Process Producing Monoclonal Antibodies Using In-House Media....Pages 659-661
Production of an Anti β2 Integrin Monoclonal Antibody by a Hybridoma Cell Line Grown in a 2 Liter Bioreactor....Pages 663-666
Economic Evaluation of Disposables vs Stainless Steel within a Modular Pilot Plant Facility — a Comparative Analysis....Pages 667-670
CGMP Manufacturing of a Fusion Protein in Mammalian Cells Using a Large-Scale Acoustic Perfusion System....Pages 671-674
Influence of Culture Parameters on Viability in Perfusion Process....Pages 675-677
Use of Hydrocyclone as an Efficient Tool for Cell Retention in Perfusion Cultures....Pages 679-682
Effect of Different Variables on the Long-Term Spinfilter Clogging during Pilot-Scale Animal Cell Perfusion Runs....Pages 683-685
Integrating Acoustic Perfusion in Mammalian Cell Culture: Temperature Control....Pages 687-691
Flexible Fed-Batch Process Control of Animal Cells....Pages 693-695
Development of a Fed-Batch Strategy for the Production of NS0-Derived Humanised Mab in Protein Free Medium....Pages 697-700
Transfection to Manufacturing: Reducing Timelines for High Yielding GS-CHO Processes....Pages 701-704
Optimization of a Fed-Batch Process Producing Humanized Antibodies....Pages 705-709
Optimized Feeding Strategy for NS0 Cells....Pages 711-714
Comparison of Complementary Interactions between Synthetic Ligands and t-PA by Hpmdc....Pages 715-717
Effect of Nutrient Supplementation on the Biological Quality of tPA Produced by CHO Cells on Serum-Free Medium....Pages 719-722
Modulation of the Glycosylation Repertoire of a Recombinant Human EPO Expressing Model Cell Line Under Different Culture Conditions....Pages 723-725
Physicochemical Estimation of rhEPO Potency Produced in Suspension Culture....Pages 727-729
Post-Translational Modification of Recombinant Human GM-CSF Expressed by Stable-Transfected CHO Cells under Different Culture Conditions....Pages 731-734
Softmix, a new Scalable Mixing System for the Cultivation of Sensitive Cells on Microcarrier under Protein- Free Conditions....Pages 735-738
Establishment of Fermentation Process for Hrepo Using Stirred Tank Fermenters in Perfusion Mode Using A Commercial Protein-Free Culture Medium....Pages 739-742
Continuous Gmp Production in Hollow Fibre Bioreactors — Aviable Alternative for the Production of Low Volume Biopharmaceuticals....Pages 743-745
Optimizing Cultivation Strategies in Different Scales of Hollow Fiber Bioreactors....Pages 747-750
Increasing Antibody Production from Hollow Fiber Systems....Pages 751-753
Production of Poliovirus, Rubella Virus, Measles Virus and Recombinant Adenovirus in Protein-Free Media....Pages 755-759
Serum-Free Production of Poliovirus: A Comparative Study Using Microcarriers, Roller Bottles and Stationary Cell Culture....Pages 761-765
Glutamine-Free Media for Vaccine Production Processes....Pages 767-770
Serum Free Cultivation of Primary Chicken Embryo Fibroblasts in Microcarrier Systems for Vaccine Production....Pages 771-774
Design of an Animal Protein Free Medium to Sustain Vero Cells Growth in a Stirred Tank Bioreactor....Pages 775-778
Use of On-Line Our Measurements to Monitor and Improve Insect Cell Cultures for Recombinant Protein Production....Pages 779-782
Production of Live Influenza Vaccine in MDCK Cell Line....Pages 783-785
Elaboration of the Microencapsulated Forms of the Viral Vaccines....Pages 787-789
HTS-Based Development of High-Producing CHO Cell Lines....Pages 791-792