Analytical Use of Fluorescent Probes in Oncology

Front Matter....Pages i-x
Overview....Pages 1-23
Detection of Breast Tumors by Absorption and Fluorescence of Photon Diffusive Waves....Pages 25-30
Fluorescence-Lifetime Based Sensing in Tissues and Other Random Media with Measurements of Photon Migration....Pages 31-40
Spectroscopy and Tomography of Tissues in the Frequency-Domain....Pages 41-52
Fret Microscopy in Cellular Signal Transduction....Pages 53-57
Photoacoustic Spectroscopy Applied to Biological Materials....Pages 59-64
Recent Developments in Fluorescence Spectroscopy....Pages 65-79
Photonic Pathology....Pages 81-89
 In Vivo Application PD-Porphine for Measurement of Oxygen Concentrations in the Gut....Pages 91-96
Laser Scanning Confocal Fluorescence Microscopy of Cell Function....Pages 97-111
Sub-Cellular Localization of Second Generation PDT Photosensitizers Studied by Microspectrofluorometry....Pages 113-121
Photofrin Uptake as a Function of Tumor Size and the Detection of Microscopic Nests of Tumor Cells by Photofrin Fluorescence....Pages 123-128
The Detection of Apoptotic Cells and Their Quantification by Flow Cytometry....Pages 129-135
Adhesion Stabilization of Blood Borne Cancer Cells in the Microcirculation....Pages 137-148
Probing of Connexin Channels....Pages 149-156
The Analysis of Drug-Induced Cell Cycle Perturbations by Flow Cytometry....Pages 157-172
The Effects of Tumor Heterogeneity on the Flow Cytometric Analysis of Clinical Specimens....Pages 173-179
Videomicrofluorometry of Progesterone Receptors and Their Genes in Breast Cancer Cells....Pages 181-188
Immunophotodetection of Cancer by Antibody-Indocyanin Conjugates....Pages 189-204
Three-Dimensional Optical Functional Imaging of Tissue with Two-Photon Excitation Laser Scanning Microscopy....Pages 205-211
Estrogen Receptor Imaging Using Intrinsically Fluorescent Ligands....Pages 213-219
Videomicrofluorometry and Intracellular Ionic Concentration in Single Living Cells....Pages 221-229
Multiwavelength MicroFluorometry to Investigate Cytotoxic Processes on Sensitive and Resistant Human Lymphoblastoid CCRF-Cem Lines....Pages 231-239
Laser-Induced Auto Fluorescence of Normal and Tumor Bladder Cells and Tissues....Pages 241-247
Fluorescent Imaging of Membrane Potential Components....Pages 249-256
Probing of Microtubule Structures with Monoclonal Antibodies and Immunofluorescence Microscopy....Pages 257-264
Immunofluorescence Detection of Cytoskeletal Structures....Pages 265-271
Cell Expression System in V79 Cells Transfected with Cytochrome P450 and its Performance in Metabolism Studies....Pages 273-282
Direct Transfer of Control and Multidrug Resistance....Pages 283-292
Instrumentation Design for Study of Metabolic Control in Living Cells....Pages 293-298
Use of Fluorescent Derivatives for Labeling Cells; Diagnosing, Preventing, and Correcting Genetic Diseases; Photosynthesizing and Killing of Tumors, Parasites, and Viruses....Pages 299-308
Ca 2+ Signaling and Cytoskeleton Reorganization in Endothelial Cells Stimulated by Bradykinin....Pages 309-315
Integration of Optical Techniques in Scanning Probe Microscopes....Pages 317-323
Perfusion, Temperature, and PO 2 Control in a Leiden Culture Bath for Single Cell Measurements and Manipulations....Pages 325-330
Immunofluorescence Study of Intermediate Filament Protein Expression under Normal and Pathological Conditions....Pages 331-337
The Use of Fluorescent Probes in Characterizing Drug Resistance in Cultured Cells....Pages 339-347
A High Resolution Multimode Digital Imaging System for Fluorescence Studies of Mitosis....Pages 349-356
Use of Photodiode Array Fluorescence Spectroscopy in Biochemistry, Pharmacology, and Oncology....Pages 357-362
Hydrophobic Photosensitizers Delivery to Tumor Tissues by an Amphiphilic Peptide....Pages 363-370
Merocyanine 540—A Fluorescent Dye and a Biological Probe....Pages 371-382
High Pressure Infrared Spectroscopy in Biological and Biomedical Studies....Pages 383-392
Fluorescence Spectroscopy for Diagnosis of Cancerous Tissues....Pages 393-404
Solvent-Dependent Excited State Properties of Psoralens....Pages 405-409
Fluorescent Probes in Breast Tumor....Pages 411-417
Use of Fluorescence in Situ Hybridization for Mapping and Ordering YAC Clones from Chromosomal Regions with Suspected Tumor-Suppressor Activity....Pages 419-421
Photochemical and Photophysical Studies of an Anthocyanin....Pages 423-424
Selectivity and Localization of Anthracyclines in Cardiac-Muscle and Non-Muscle Cells Relevance to Multidrug Resistance....Pages 425-426
Back Matter....Pages 427-448