Alternatives for Dermal Toxicity Testing

Front Matter ....Pages i-xx
Front Matter ....Pages 1-1
 Overview on Current Status of Alternative Methods and Testing Approaches for Skin Irritation Testing  (Chantra Eskes, Markus Hofmann)....Pages 3-23
 Skin Irritation Hazard of Chemicals Assessed by the EpiSkin™ In Vitro Test Method  (Nathalie Alépée, Marie Hélène Grandidier, José Cotovio)....Pages 25-39
 The EpiDerm™ Skin Irritation Test (EpiDerm™ SIT)  (Helena Kandarova, Manfred Liebsch)....Pages 41-57
 An In Vitro Skin Irritation Test Using the SkinEthic™ Reconstructed Human Epidermal (RHE) Model  (Nathalie Alépée, Marie Hélène Grandidier, Carine Tornier, José Cotovio)....Pages 59-72
 In Vitro Skin Irritation Assay with the LabCyte EPI-MODEL  (Hajime Kojima, Masakazu Katoh)....Pages 73-80
 The epiCS® Skin Irritation Test (SIT) Method  (Oliver Engelking, Horst W. Fuchs, Dirk Weisensee)....Pages 81-86
Front Matter ....Pages 87-87
 Overview on Current Status of Alternative Methods and Testing Approaches for Skin Corrosion Testing  (Chantra Eskes, Markus Hofmann)....Pages 89-106
 The EpiSkin™ Human Epidermis Model for In Vitro Skin Corrosion of Test Chemicals  (Nathalie Alépée, Marie Hélène Grandidier, José Cotovio)....Pages 107-125
 The EpiDerm™ Skin Corrosion Test (EpiDerm™ SCT)  (Helena Kandarova, Manfred Liebsch)....Pages 127-142
 Assessment of the Human Epidermis SkinEthic™ RHE Model for In Vitro Skin Corrosion Testing of Chemicals  (Nathalie Alépée, Marie-Hélène Grandidier, José Cotovio)....Pages 143-157
 The epiCS® Skin Corrosion Test Method  (Oliver Engelking, Dirk Weisensee, Horst W. Fuchs)....Pages 159-166
 The In Vitro Membrane Barrier Test Corrositex® for Skin Corrosion  (Rich Ulmer, Amy Wang)....Pages 167-173
 The Rat Skin Transcutaneous Electrical Resistance (TER) Test  (Robert Guest)....Pages 175-195
Front Matter ....Pages 197-197
 Overview on Current Status and Combination of Test Methods  (Erwin L. Roggen)....Pages 199-214
 The Local Lymph Node Assay  (David A. Basketter, Ian Kimber, G. Frank Gerberick)....Pages 215-223
 Utilization of Peptide Reactivity Assays for the Prediction of Skin Sensitization  (G. Frank Gerberick, John A. Troutman, Leslie M. Foertsch, Petra S. Kern)....Pages 225-233
 The KeratinoSens™ Assay for Skin Sensitization Screening  (Andreas Natch)....Pages 235-248
 LuSens: Shedding Light on Skin Sensitization  (Tzutzuy Ramirez, Annette Mehling, Robert Landsiedel)....Pages 249-262
 NCTC 2544 and IL-18 Production: A Tool for the Identification of Contact Allergens  (Emanuela Corsini, Valentina Galbiati)....Pages 263-272
 Epidermal Equivalent (EE) Potency Assay  (Susan Gibbs, Sander W. Spiekstra)....Pages 273-287
 The h-CLAT Method  (Hitoshi Sakaguchi, Takao Ashikaga)....Pages 289-309
 U-SENS™: A U937 Cell Line Activation Test for Skin Sensitization  (Nathalie Alépée, Cécile Piroird, Laurent Nardelli)....Pages 311-330
 Human Peripheral Blood Monocyte Derived Dendritic Cells Assay for the Detection and Characterization of Sensitizers  (Andreas Schepky, Hendrik Reuter, Jochen Kühnl, Pierre Aeby)....Pages 331-345
 VITOSENS™  (Nathalie Lambrechts, Greet Schoeters, Rosette Van Den Heuvel, Hilda Witters, Inge Nelissen, Jef Hooyberghs)....Pages 347-359
 The SENS-IS Assay  (Françoise Cottrez, Elodie Boitel, Claude Auriault, Hervé Groux)....Pages 361-375
 The SenCeeTox® Assay  (Donald Keller, Caroline Bauch, Prakash Patel)....Pages 377-391
 GARD: Genomic Allergen Rapid Detection  (Malin Lindstedt, Kathrin Stephanie Zeller, Henrik Johansson, Carl Borrebaeck)....Pages 393-403
 Proteomics Testing for Sensitization Potency Using the Sensiderm™ TMT-SRM 10-Plex Assay  (Petra Budde, Hans-Dieter Zucht, Ian Pike)....Pages 405-416
 In Vitro Dendritic Cell-Based Test for Skin Sensitizers Identification and Potency Estimation  (Bruno Miguel Neves, João Demétrio Martins, Isabel Ferreira, Ana Silva, Maria Teresa Cruz)....Pages 417-435
 An In Vitro Human Skin Test for Assessing Adverse Immune Reactions and Sensitization Potential  (Anne Dickinson, Xiao Nong Wang, Shaheda Ahmed)....Pages 437-448
 The Human T Cell Priming Assay (hTCPA)  (Philipp R. Esser, Stefan F. Martin)....Pages 449-454
 Promising Test Systems Beyond the Current Status  (Erwin L. Roggen)....Pages 455-460
Front Matter ....Pages 461-461
 Overview on the Current Status of Available Test Methods and Additional Promising Methods for Assessing UV-Induced Effects  (Lorena Rigo Gaspar, Camila Martins Kawakami, Carolina Gomes Benevenuto)....Pages 463-476
 Reactive Oxygen Species Assay for Evaluating Phototoxicity Potential  (Hajime Kojima, Kazuhiro Hosoi, Satomi Onoue)....Pages 477-482
 The EpiDerm™ Phototoxicity Test (EpiDerm™ H3D-PT)  (Helena Kandarova, Manfred Liebsch)....Pages 483-503
Front Matter ....Pages 505-505
 Overview on Current Status  (Stefan Pfuhler, Kerstin Reisinger)....Pages 507-512
 Reconstructed Skin Micronucleus Assay (RSMN)  (Stefan Pfuhler, Kerstin Reisinger)....Pages 513-525
 3D Skin Comet Assay  (Kerstin Reisinger, Stefan Pfuhler)....Pages 527-539
 Role in a Testing Strategy  (Kerstin Reisinger, Stefan Pfuhler)....Pages 541-544
Front Matter ....Pages 545-545
 Progenitor Skin Cell Therapy and Evolution of Medical Applications  (Lee Ann Applegate, Paris Jafari, Corinne Scaletta, Anthony de Buys Roessingh, Wassim Raffoul, Nathalie Hirt-Burri)....Pages 547-564
 Integrated Safety Strategy for the Development of Children’s Cosmetic Products Using In Vitro and Clinical Methodologies  (Andrezza di Pietro Micali Canavez, Talita M. T. P. Silveira, Natalia de Albuquerque Vita, Ana Cristina Weihermann, Caroline Radoski Neumann, Desirée Cigaran Schuck et al.)....Pages 565-577
 High-Throughput Screening Techniques  (Rico Brendtke, Bart De Wever, Florian Groeber, Jan Hansmann, Freia Schmid, Heike Walles)....Pages 579-592