Alpha-Synuclein: Methods and Protocols

Front Matter ....Pages i-xii
 Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons  (Laura A. Volpicelli-Daley)....Pages 1-14
 Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue  (John B. Sanderson)....Pages 15-22
 Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons  (Karina J. Vargas)....Pages 23-33
 Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform  (Luis Concha-Marambio, Mohammad Shahnawaz, Claudio Soto)....Pages 35-44
 Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain Using Preformed Fibrils  (Bin Zhang, Victoria Kehm, Ron Gathagan, Susan N. Leight, John Q. Trojanowski, Virginia M.-Y. Lee et al.)....Pages 45-57
 Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection  (Niels Kruse, Brit Mollenhauer)....Pages 59-68
 Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers  (Rosalind F. Roberts, Nora Bengoa-Vergniory, Javier Alegre-Abarrategui)....Pages 69-76
 Studying α-Synuclein Conformation by Intact-Cell Cross-Linking  (Thibaut Imberdis, Saranna Fanning, Andrew Newman, Nagendran Ramalingam, Ulf Dettmer)....Pages 77-91
 C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques  (Mandy Koopman, Renée I. Seinstra, Ellen A. A. Nollen)....Pages 93-112
 Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein  (Tara A. Eastwood, Daniel P. Mulvihill)....Pages 113-121
 Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes  (Matteo Rovere)....Pages 123-143
 Yeast-Based Screens to Target Alpha-Synuclein Toxicity  (Inês Caldeira Brás, Blagovesta Popova, Gerhard H. Braus, Tiago F. Outeiro)....Pages 145-156
 Probing Structural Changes in Alpha-Synuclein by Nuclear Magnetic Resonance Spectroscopy  (Tapojyoti Das, David Eliezer)....Pages 157-181
 Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers  (Eric S. Luth, Irina G. Stavrovskaya)....Pages 183-198
 Monitoring α-Synuclein Proteotoxicity in Drosophila Models  (Aron Szabo, George K. Tofaris)....Pages 199-208
 Detection of α-Synuclein in Biological Samples Using Mass Spectrometry  (Ann Brinkmalm, Annika Öhrfelt, Payel Bhattacharjee, Henrik Zetterberg)....Pages 209-220
 Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation  (Mahdi Muhammad Moosa, Josephine C. Ferreon, Allan Chris M. Ferreon)....Pages 221-233
 In Situ Peroxidase Labeling and Mass Spectrometry of Alpha-Synuclein in Rat Cortical Neurons  (Ricardo Sanz, Patrick Ovando-Roche, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung, Vikram Khurana)....Pages 235-246
 Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy  (Julia Cattani, Theresa Braun, Malte Drescher)....Pages 247-260
 Expression and Purification of Untagged α-Synuclein  (Alex E. Powers, Dushyant S. Patel)....Pages 261-269
 Generation and Analysis of Viral Vector-Mediated Rodent Models for Parkinson’s Disease  (Akua A. Karikari, James B. Koprich, Chi Wang Ip)....Pages 271-286
Back Matter ....Pages 287-289