Advances in Forensic Haemogenetics: 15th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Venezia, 13–15 October 1993

Front Matter....Pages I-XXVII
Front Matter....Pages 1-1
History and Geography of Human Genes....Pages 3-12
Front Matter....Pages 13-13
DNA — A Human Rights Profile....Pages 15-22
The Association of Individual Sociodemographic Factors with Paternity Test Results....Pages 23-25
The Expert’s Evidence and the Judge’s Evaluation of all Information in Court Decisions....Pages 26-31
Ethical Considerations About Unexpected Facts Disclosed during Genetic Association Studies....Pages 32-34
Front Matter....Pages 35-35
Molecular analysis of classical red cell markers....Pages 37-44
The Molecular Genetics of HLA....Pages 45-49
A New Look at Old Friends: The Molecular Biology of the Protein Markers....Pages 50-56
The Molecular Basis of The “Red Cell” Acid Phosphatase Polymorphism....Pages 57-64
Human Red Cell Acid Phosphatase (ACP1): Genetic Typing at the DNA Level....Pages 65-67
Molecular Analysis of Esterase D Polymorphism....Pages 68-70
Front Matter....Pages 71-71
Quality Assessment as an Essential Tool for Tile Achievement of Total Quality....Pages 73-80
Quality control of paternity investigations in Scandinavia....Pages 81-83
Comparison of DNA-Profiling and Classical Blood Grouping in Criminal Cases....Pages 84-86
Fluorescent Based Typing of Two Short Tandem Repeat Loci in a Swedish Population Sample and Reproducibility of Fragment Size Estimates....Pages 87-89
Restriction Fragment Length Polymorphism: Image Analysis and Molecular Weight Calculation with a Scanner-Based Computer System....Pages 90-92
Quality Control: Interlaboratory Comparison of RFLP Results....Pages 93-95
An Optimised Chemiluminescent Detection System: Is It Compatible with Data Generated Using 32 P and a Different Ladder?....Pages 96-100
Front Matter....Pages 101-101
DNA Variation and Its Analysis by Hybridisation to Oligonucleotide Arrays....Pages 103-108
Simultaneous analysis of STR and VNTR polymorphisms....Pages 109-111
Front Matter....Pages 101-101
Streamlining VNTR Analysis. A Fast Procedure for Non Isotopic DNA Profiling....Pages 112-114
Nonradioactive Approach to Type DNA by Minisatellite Variant Repeats....Pages 115-117
Minisatellites of the Y Chromosome....Pages 118-120
Anomalous Electrophoretic Behaviour of HUMACTBP2 (SE33)....Pages 121-123
Evaluation of the Tris-Glycine/Tris-Chloride Discontinuous Buffer System for the Electrophoretic Analysis of VNTR and STR Loci....Pages 124-126
Multilocus Profiles of the Basque Country Population with Dig-Labelled Probes 33.15 and 33.6....Pages 127-129
Use of Chemiluminescent Labeled Probes for Forensic and Paternity Determinations....Pages 130-132
Three VNTR and Y-Chromosome Identification from Biological Stains....Pages 133-135
Application of the Capillary DNA Chromatography in the Paternity Testing Using APOB Amplified Alleles....Pages 136-138
Automated Laser Fluorescent Analysis of Amplified Fragment Length Polymorphisms and Short Tandem Repeats....Pages 139-141
Further Characterization of the VNTR Probe LH1 (D5S110) and Applications for DNA Typing....Pages 142-144
Method for the Evaluation of C-Bands Heteromorphism in Human Chromosomes by Image Processing (I.P.)....Pages 145-147
Polymorphism of the D1S80 Locus in Basque Country Population and Its Forensic Applications....Pages 148-150
Studies on Sex Determination with Different Y-Specific Probes After Quantifying Human Genomic DNA with the Slot Blot Hybridization Method....Pages 151-155
Typing Human DNA Using Capillary Electrophoresis: Comparison of Slab Gel and Capillary Formats....Pages 156-159
Comparison of DNA-Extraction Methods from Compact Bone Tissue....Pages 160-163
DNA-Profiling on carpeting — Methods of purification, restriction and detection....Pages 164-166
DNA Extraction for PCR: Phenol/Chloroform vs. Chelex — A Comparative Study....Pages 167-169
Simple and Rapid Typing for VNTR Polymorphisms Using High Resolution Electrophoresis of PCR Products on Rehydratable Polyacrylamide Gels....Pages 170-172
DNA Typing of Coagulation Factor XIII “a” Subunit by PCR-RFLP and SSCP....Pages 173-175
Front Matter....Pages 101-101
Two Different Mechanisms are Involved in the Polymorphism Shown by the Locus D2S44....Pages 176-178
Front Matter....Pages 179-179
Paternity Testing by Oligonucleotide DNA Fingerprinting: A Multi-Center Study Proving Reliability and Validity....Pages 181-183
Population Genetic Studies of Short Tandem Repeat Loci (STRS): Efficiency in Paternity Testing....Pages 184-186
Application of a Y-linked tetranucleotide repeat polymorphism to paternity cases....Pages 187-189
Clarification of a Paternity Case with the Mother, Two Children and the Mother of the Putative Father....Pages 190-192
Analysis of the Locus D1S80: Frequency Distribution in North-East England and Application to Paternity Testing....Pages 193-195
An Unusual Case of Failed Vasectomy Confirmed by DNA Profiling....Pages 196-198
Development and Validation of Buccal Swab Collection Method for DNA Testing for Paternity Testing....Pages 199-201
Application of Conventional and DNA Polymorphism to Paternity Cases....Pages 202-203
DNA Typing — Strategies in Paternity and Forensic Casework....Pages 204-206
Parentage Control in Cattle by Genotyping Microsatellites....Pages 207-209
Paternity Testing with DNA Systems: Application of D1S80 Phenotyping to Danish Paternity Cases Analysed with Five VNTR Single Locus Systems....Pages 210-213
PCR Polymorphisms Compared to Others Genetic Markers in the Paternity Testing....Pages 214-216
Case Report of a Disputed Paternity with No Biological Sample from the Putative Father....Pages 217-219
Paternity testing of endangered species of birds by DNA fingerprinting and random amplified polymorphic DNA fingerprinting....Pages 220-222
Characterization of Three Microsatellites and Their Application in Paternity Testing....Pages 223-225
Paternity Testing in Italy Using Minisatellite Variant Repeat (MVR)....Pages 226-228
PCR based analysis of HLA-DQα, D1S80 and Apo B loci in paternity testing....Pages 229-231
Proving Paternity Using Formalin Fixed, Paraffin Embedded Tissue Samples from a Deceased Father....Pages 232-234
Application of Single Locus Probes in Cases of Disputed Paternity — The Usefulness of the Combination of HLA and DNA....Pages 235-239
Front Matter....Pages 179-179
Expertise Report in Disputed Paternity Cases with Two or More Children....Pages 240-241
Front Matter....Pages 243-243
RFLP Typing: A New Highly Polymorphic VNTR Locus and Chemiluminescent Detection....Pages 245-252
Evaluation of Amp-FLP Markers and Summary of PCR-Based Forensic Casework....Pages 253-255
Investigations on vaginal cell/sperm mixtures from microscopical slides....Pages 256-258
Dealing with Human Remains Sampled in Disaster Areas the Case of the Israelian Embassy Explosion Ocurred in Buenos Aires....Pages 259-261
Casework Experience with Single Locus DNA Probes and Chemiluminescent Detection in Spain....Pages 262-264
DNA Analysis of Human Blood Recovered from Explosion Debris....Pages 265-267
Genetic Identification from Dental Pulp by Using DNA Amplification (PCR)....Pages 268-270
The degradation of a VNTR polymorphic site in various biological specimens when subjected to different temperature conditions....Pages 271-274
About a Case of DNA Analysis from Some Latent Blood Stains on Overalls Found in a River....Pages 275-277
Forensic DNA-typing in the Netherlands using VNTR Single Locus Probes....Pages 278-281
The Use of DNA Profiling in the Identification of Victims of an Aircrash....Pages 282-285
CODIS: A National Index of DNA Identification Records....Pages 286-288
 Post-Mortem Molecular Identification. Biological Kinship Established by DNA Analysis.....Pages 289-291
Application of PCR Technique for the Characterization of Human Bones....Pages 292-294
Use of PCR for Forensic Analysis of DNA in Cigarette Ends....Pages 295-297
Quantisation of Mixed Chimerism in Blood and Bone Marrow....Pages 298-301
PCR Amplification of DNA from Old Blood Stains....Pages 302-303
Blood Group Typing and PCR-Analysis in Stored Blood Samples....Pages 304-306
Front Matter....Pages 307-307
Automated DNA Profiling Employing Multiplex Amplification of Short Tandem Repeat Loci....Pages 309-311
Front Matter....Pages 307-307
An Evaluation of Automated fluorescent PCR-Based DNA Typing....Pages 312-316
Comparison of Manual and Automated Detection for STRs Analysis....Pages 317-319
Evaluation of native and denaturing polyacrylamide gel electrophoresis for short tandem repeat analysis....Pages 320-322
Analysis of the Short Tandem Repeat Polymorphism SE33: A New High Resolution Separation of SE33 Alleles by Means of Direct Blotting Electrophoresis....Pages 323-325
Analysis of the COL2A1 Polymorphism in a Small Pan troglodytes Population, and Its Forensic Application....Pages 326-328
DNA Sex Test: A New Rapid and Quantitative Forensic Approach Using Amelogenin Gene Based Fluorescent PCR....Pages 329-332
DNA Typing on Single Hair — Recent Possibilities Based on New Extraction Method....Pages 333-335
Extraction and PCR amplification of DNA from hair shafts....Pages 336-338
Forensic application of SSCP in the analysis of HLA-DQ, which was amplified by PCR....Pages 339-342
PCR DNA Typing of Washed Stains....Pages 343-345
Genetic Studies of a STR at the vWF Locus and Its Application to Individualisation....Pages 346-348
HLA-DQα Typing by DNA Amplification of Single Human Hair....Pages 349-350
Comparison of two methods for HLA-DQA1 typing....Pages 351-353
Rapid Air Cycling of D1S80 Amplification....Pages 354-356
PCR-Based Analyses for Identity Testing....Pages 357-359
PCR Amplification of the D1S80 Locus: Analysis Using Rehydratable Horizontal Polyacrylamide Gels....Pages 360-362
Random Priming and Multiplex PCR with Three Short Tandem Repeats in Forensic Caseworks....Pages 363-365
Improved Conditions for Genotype Diagnosis of a STR of the hTPO Locus....Pages 366-368
Sex Determination in Forensic Samples by PCR: A Comparison of Two Alternative (AMGL/AMG and DYZ3/DXZ1) Markers.....Pages 369-371
PCR on Reusable Matrix-Fixed DNA....Pages 372-374
Front Matter....Pages 307-307
Effect of Degradation on PCR Based DNA Typing....Pages 375-378
Use of PCR in Forensic Casework in Southern Spain.....Pages 379-381
Advantages and Disadvantages of the Application of Nested PCR for Typing of Forensic Samples....Pages 382-386
Suitability and Efficiency of PCR Systems in Forensics....Pages 387-389
The USE of Capillary Electrophoresis in Forensic DNA Analysis....Pages 390-392
PCR inhibitor: water-soluble melanin, which inhibits DNA polymerases and DNases....Pages 393-396
Front Matter....Pages 397-397
Identification of Human Remains Using Mitochondrial DNA Sequencing: Potential Mother-Child Mutational Events....Pages 399-406
Genomic Sequencing of Alleles from Str Loci.....Pages 407-409
Evidence for a Mutation at 3’hvr COL2A1 Locus—Segregation Analysis and Sequence Data....Pages 410-411
Isolation of a (ATTTT)n Positive Locus by Cloning, PCR Cycle Sequencing and Nonradioactive Direct Blotting Electrophoresis....Pages 412-414
Molecular Analysis of a Novel HLA-DQB1 Allele and Its Gene Frequency Among Sardinians....Pages 415-417
STR-Polymorphisms in non-human Primates....Pages 418-420
PI*Mpalermo: A New Alpha-1-Antitrypsin Deficiency Allele Detected by DNA Sequence Analysis in Two Families....Pages 421-423
Molecular Basis for Polymorphism of the “a” Subunit of Coagulation Factor XIII....Pages 424-426
High Resolution Analysis by PCR on an Automated DNA Sequencer of Internal Variation at a Pseudoautosomal VNTR (DXYS17)....Pages 427-429
Digoxigenine Labeled Sequencing of the HPRT Gene....Pages 430-432
Front Matter....Pages 433-433
The Paternity Index, Population Heterogeneity, and the Product Rule....Pages 435-437
An Italian Collaborative Study on the HLA-DQA1 Locus (GEFI’s “Garda 2” Project)....Pages 438-440
Estimation of Genetic Difference between Subpopulations Using DNA Profiling Data from Crime Investigations....Pages 441-443
Semiparametric Density Estimation with Applications to DNA Profiling....Pages 444-446
Front Matter....Pages 433-433
Bioassay of kinship using VNTR alleles....Pages 447-449
Analysis by PCR/Oligonucleotide Typing of HLA Class II Alleles in a Variety of Human Populations....Pages 450-452
Evaluation of the Product Rule....Pages 453-455
Population Studies in Turkey....Pages 456-462
Allele Frecuency Distributions of Five VNTR Loci (D1S7, D7S21, D12S11, D5S43 and D2S44) in Spain....Pages 463-465
Allelic Heterogeneity of Single Locus Probes in the Populations of the Basque Country, Spain: Application in Paternity Testing....Pages 466-468
Analysis of the Short Tandem Repeat Polymorphism TC 11 (HUMTH01): Allele Frequencies and Family Studies....Pages 469-471
Distribution of HLA-DR and -DQ Alleles Revealed by Restriction Fragment Length Polymorphism Analysis in Donors from St. -Petersburg....Pages 472-474
Allele Frequencies of the vWA Str Locus in Italy....Pages 475-477
Allelic Frequency in the Population of Spain Using Three Hypervariable Loci....Pages 478-480
Polymorphism of the 3′-ApoB VNTR system in a population sample from the province of Parma....Pages 481-483
Allele Frequencies of Three STRs in an Italian Population Sample....Pages 484-486
Population Genetics of VNTR Markers (TPO and 3’APOB Loci) in the Mediterranean Area (Albania, Greece and Italy)....Pages 487-489
Distribution of HLA Class II Genes in a Caucasian Population as Determined by PCR and Reversed-Dot-Blot Typing....Pages 490-492
Study of the APO B Polymorphism in Tuscany (Italy)....Pages 493-495
The Distribution of HUMTH01 Polymorphism in Northern and Central Italy....Pages 496-498
HLA-DQA1 Polymorphism in a Portuguese Population....Pages 499-501
Suitability of the ACTBP2 (SE33) STR-Polymorphism for Legal Medicine Investigation in the Population of Catalonia (North-East Spain)....Pages 502-504
Study of Three Minisatellites (D1S80, YNZ22, 3′ApoB) Performed by P.C.R. in the Population of Catalonia (North-East Spain)....Pages 505-507
Population Genetics of Three STR Polymorphisms in a Chinese Population....Pages 508-510
Front Matter....Pages 433-433
Comparison of Different Tests for Deviation from Hardy-Weinberg Equilibrium of AMPFLP Population Data....Pages 511-514
Swiss Population Data for 3 STR-Systems (SE33, HUMTHO1, D21S11), HLA DQα and D1S80....Pages 515-517
RFLP and STR Polymorphisms in a Swiss Mountain Population....Pages 518-520
False Paternity Trios in white, black and Cape Coloured populations....Pages 521-523
APOB 3′ VNTR Polymorphism in Southern African Populations....Pages 524-526
Spanish Population Data for the Short Tandem Repeat ACTBP2 (SE33)....Pages 527-529
Study of allele frequencies distribution of the 3′-HVR marker near α -globin gene in the french population....Pages 530-532
HLA-DQA1 Subtyping by PCR Followed by a Combined SSO/RFLP Method of Detection. Distribution of Alleles and Genotypes in two Spanish Populations....Pages 533-535
A UK Caucasian Database for the TBQ7 (D10S28) Locus Derived from Blood Samples Submitted for Paternity Analysis....Pages 536-538
Four STRs in 300 Norwegians....Pages 539-541
French Population Data of 6 AMPFL’S....Pages 542-544
Gardal (D2S44 SBA) — Harmonization of Protocols and a Collaborative Database....Pages 545-546
APO B Polymorphism in the District of Bologna (Italy)....Pages 547-549
HLA.DQ-Alpha and D1S80 Data from a French Caucasian Population Random Sample Versus a Sample of Aircrash Victims....Pages 550-553
Investigation of the STR locus HUMTH01 using PCR in Caucasian samples from England and Galicia, NW Spain....Pages 554-558
The Distribution of HLA DQA1 Alleles in the Population of the North of Portugal....Pages 559-561
Study of D1S80 Locus Polymorphism in the North of Portugal....Pages 562-564
Genetic Substructure of the Italian Population at the VNTR Loci D1S80 and D17S30: The Tuscan Region....Pages 565-568
Allele Frequencies at the D2S44, D10S28 and D14S13 Loci in Four Southern African Populations....Pages 569-571
HLA DQA1 and D1S80 Polymorphisms in the Population of Andalusia (Southern Spain)....Pages 572-574
Front Matter....Pages 433-433
Studies on the HUMFABP and HUMTH01 Polymorphisms....Pages 575-577
Phenotype and Allele Frequencies of 4 VNTR-AMPFLP’s in an Austrian Population Sample....Pages 578-580
Typing for STR-loci by Electrophoresis on Rehydratable Polyacrylamide Gels: Phenotype and Allele Frequencies of SE33 and TC11 in an Austrian Population Sample....Pages 581-583
Allele Frequency Distribution in Schleswig-Holstein for the DNA Probes MS1, MS31, MS43A, G3 and YNH24....Pages 584-586
Allele Frequencies of the HUMTH01 Locus in the Italian Population....Pages 587-589
PCR Typing of the COL2A1 System: Allelic Frequencies in Two Population Samples from North and Central Italy....Pages 590-592
Deoxyribonucleic Acid (DNA) HLA - DQ α Allele Frequency Distribution in Various Sects of South Indian Population....Pages 593-595
Distribution of HLA-DQα Alleles in Deoxyribonucleic Acid (DNA) from Caucasian and Black Populations of Marion County, Indiana, USA....Pages 596-598
HLA-DQα PCR System: Frequencies of a South Bavarian Population and Family Data....Pages 599-602
Front Matter....Pages 603-603
Subtyping of coagulation factor XIIIA by Isoelectric focussing....Pages 605-607
Haptoglobin Subtypes in Tuscany (Italy)....Pages 608-610
Haptoglobin Subtypes in Several Populations of Spain....Pages 611-613
GC, TF and PI Polymorphisms in Madeira (Portugal) PI*S Allele and Paternity Testing....Pages 614-616
Further Evidence for Transformation of Genetic Markers in Recipients after BMT....Pages 617-619
Two Cases of Duplication of the 1F and 1A2 Genes in the Vitamin D-Binding Protein (GC) System....Pages 620-622
ABO Blood Grouping of Old Blood Stains by Ultra Micro Reverse Agglutination with Monoclonal Anti-A, -B Antibodies....Pages 623-625
Transferrin Subtyping in Human Organ Tissues....Pages 626-628
Serum Protein Detection in Old Dental Pulp....Pages 629-631
Genetic Diversity in Sri Lanka: Some implications in Paternity and Forensic testing....Pages 632-634
Human Inter-Alpha-Trypsin Inhibitor (ITI) Silent Allele Found in a Case of Disputed Paternity....Pages 635-637
Front Matter....Pages 603-603
Lectin- and Immuno-Histochemistry on Mucous Substances of the Taste Buds and Lingual Glands in Some Mammals....Pages 638-640
Immunization of Thalassaemic Patients Against the Ag System....Pages 641-643
PI (α-1-Antitrypsin) Subtypes in NW Portugal....Pages 644-646
Comparative Lectin and Immuno-Histochemistry on Antigen Expression in Blood Group A1 and A2 Individuals....Pages 647-649
Orosomucoid Phenotyping Using Monoclonal Antibodies....Pages 650-652
Study of Rapid Determination of ABO Blood Group in Bloodstains by the Improved Dot-ELISA with Monoclonal Antibodies....Pages 653-655
Investigations on the distribution pattern of haptoglobin subtype allele frequencies in European populations — Preliminary report —....Pages 656-658
The Distribuiton of Bf Phenotypes in Human Sera in Beijing Area and Detection of Bf Phenotypes in Bloodstains....Pages 659-661