Acinetobacter baumannii: Methods and Protocols

Front Matter ....Pages i-xiv
 Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp.  (Binesh Lal, Saranya Vijayakumar, Shalini Anandan, Balaji Veeraraghavan)....Pages 1-15
 Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance  (Saranya Vijayakumar, Balaji Veeraraghavan, Agila K. Pragasam, Yamuna Devi Bakthavachalam)....Pages 17-22
 Antimicrobial Susceptibility Testing Methods for Acinetobacter spp.  (Balaji Veeraraghavan, Saranya Vijayakumar, Agila K. Pragasam, Yamuna Devi Bakthavachalam, John A. J. Prakash)....Pages 23-37
 Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii  (Edgar X. Sherman, Jessie E. Wozniak, David S. Weiss)....Pages 39-50
 Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper-Supplemented Liquid Media and on Copper-Containing Surfaces  (Caitlin L. Williams, D. Scott Merrell)....Pages 51-60
 A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy  (Nithin Sam Ravi, Raziya Fathima Aslam, Balaji Veeraraghavan)....Pages 61-67
 Transformation of Acinetobacter baumannii: Electroporation  (Mitchell G. Thompson, Süleyman Yildirim)....Pages 69-74
 Methods for Natural Transformation in Acinetobacter baumannii  (Gottfried Wilharm, Evelyn Skiebe)....Pages 75-85
 Vesicle-Mediated Gene Transfer in Acinetobacter baumannii  (Shweta Fulsundar, Sara Domingues, Kaare Magne Nielsen)....Pages 87-94
 Targeted Gene Replacement in Acinetobacter baumannii  (Indranil Biswas, Joshua Mettlach)....Pages 95-106
 RecET-Mediated Recombineering in Acinetobacter baumannii  (Ashley T. Tucker, Matthew J. Powers, M. Stephen Trent, Bryan W. Davies)....Pages 107-113
 Methods for Tn-Seq Analysis in Acinetobacter baumannii  (Larry A. Gallagher)....Pages 115-134
 Tn7-Based Single-Copy Insertion Vectors for Acinetobacter baumannii  (Kaleigh Ducas-Mowchun, P. Malaka De Silva, Rakesh Patidar, Herbert P. Schweizer, Ayush Kumar)....Pages 135-150
 Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii  (Sarah E. Anderson, Philip N. Rather)....Pages 151-157
 A Simple Static Biofilm Assay for Acinetobacter baumannii  (Indranil Biswas, Joshua Mettlach)....Pages 159-165
 BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth  (Bertrand Naudin, Anja Heins, Stéphane Pinhal, Emmanuelle Dé, Marion Nicol)....Pages 167-176
 In Vitro Motility Assays for Acinetobacter Species  (Indranil Biswas, Alexandra Machen, Joshua Mettlach)....Pages 177-187
 Desiccation Tolerance Assays for Acinetobacter baumannii  (Xun Wang, M. Stephen Trent, Bryan W. Davies)....Pages 189-194
 Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure  (Caitlin L. Williams, Heather M. Neu, Sarah L. J. Michel, D. Scott Merrell)....Pages 195-205
 PBP Isolation and DD-Carboxypeptidase Assay  (Shilpa Pal, Anindya S. Ghosh)....Pages 207-225
 Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii  (Kyle A. Tipton, Philip N. Rather)....Pages 227-231
 Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii  (Matthew J. Powers, Carmen M. Herrera, Ashley T. Tucker, Bryan W. Davies, M. Stephen Trent)....Pages 233-252
 Methods for Detecting N-Acyl Homoserine Lactone Production in Acinetobacter baumannii  (Aimee R. Paulk Tierney, Philip N. Rather)....Pages 253-258
 Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii  (William F. Penwell, Luis A. Actis)....Pages 259-270
 Skin and Soft Tissue Models for Acinetobacter baumannii Infection  (Daniel V. Zurawski, Jaideep Banerjee, Yonas A. Alamneh, Jonathan P. Shearer, Samandra T. Demons)....Pages 271-287
 Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection  (Lauren D. Palmer, Erin R. Green, Jessica R. Sheldon, Eric P. Skaar)....Pages 289-305
 Computational Prediction of sRNA in Acinetobacter baumannii  (Sankalp Arya, Vineet Dubey, Deepak Sen, Atin Sharma, Ranjana Pathania)....Pages 307-320
 Global Metabolic Analyses of Acinetobacter baumannii  (Mohd Hafidz Mahamad Maifiah, Tony Velkov, Darren J. Creek, Jian Li)....Pages 321-328
 Reverse Vaccinology Approach to Potential Vaccine Candidates Against Acinetobacter baumannii  (Fatima Shahid, Shifa Tariq Ashraf, Amjad Ali)....Pages 329-336
Back Matter ....Pages 337-338