1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies: Portorož, Slovenia, September 6 –10, 2015

Front Matter....Pages 1-18
Front Matter....Pages 1-1
About the First Industrial Scale PEF – Plants and Heinz Doevenspeck’s Role – A Historical Review....Pages 3-6
Harnessing the Structure Modifying Potential of Pulsed Electric Fields (PEF) – Food Processing Examples in Product Stabilization, Process Acceleration and Compound Extraction....Pages 7-10
Fundamental and Applied Aspects of Pulsed Electric Fields for Microbial Inactivation....Pages 11-14
How Imaging Molecule Uptake into Cells can Reveal the Mechanisms of Membrane Electropermeabilization....Pages 15-19
Tissue Reactions to Electroporation and Electrochemotherapy: Vascular Effects that have Implications in Tumor Treatment....Pages 20-23
Nanosecond Pulses and Beyond – Towards Antenna Applications....Pages 24-27
Optimal Irreversible Electroporation Techniques in the Treatment of Locally Advanced Liver and Pancreatic Cancer....Pages 28-31
Electrotransfer of Antiangiogenic shRNA against Endoglin for Effective Cancer Treatment....Pages 32-35
Abiotic Gene Transfer – A Rarity or a Ubiquity?....Pages 36-40
Front Matter....Pages 41-41
Antimicrobial Effect of Stevia Rebaudiana Bertoni against Listeria Monocytogenes in a Beverage Processed by Pulsed Electric Fields (PEFs): Combined Effectiveness....Pages 43-46
Pulsed Electric Field Technology Enhances Release of Anthocyanins from Grapes and Bioprotective Potential against Oxidative Stress....Pages 47-50
Effects of Pulsed Electric Fields on Selected Quality Attributes of Beef Outside Flat (Biceps femoris)....Pages 51-54
Study on a Solid-State Pulse Generator Based on Magnetic Switch for Food Treatments by Pulsed Electric Field (PEF)....Pages 55-59
Front Matter....Pages 61-61
Modeling Dynamic Electrical Impedance Spectroscopy Measurements on Electroporated Cells....Pages 63-66
Conductance Dynamics to Monitor and Control Cell Electropermeabilization....Pages 67-70
Electric Field Mapping in ex vivo Anisotropic Muscle Tissue Using DT-MREIT....Pages 71-74
Electrorotation as a Versatile Tool to Estimate Dielectric Properties of Multi-scale Biological Samples: from Single Cell to Spheroid Analysis....Pages 75-78
Electrical Measurements for Monitoring Electroporation....Pages 79-82
Comparison of Single-Shot Rapid Acquisition with Relaxation Enhancement and Echo Planar Current Density MRI Sequences for Monitoring of Electric Pulse Delivery in Irreversible Electroporation....Pages 83-86
Micro Electro-permeabilization System for Cell Medium Conductivity Change Measurement of Erythrocytes Cells....Pages 87-90
Front Matter....Pages 61-61
Magnetic Resonance Electrical Impedance Tomography for Monitoring Electrical Conductivity during Delivery of Electric Pulses in Irreversible Electroporation....Pages 91-94
Front Matter....Pages 95-95
Mathematical Modeling of Electrochemical Phenomena at the Electrode-Solution Interface in a PEF Treatment Chamber....Pages 97-100
Electrolytic Ablation Dose Planning Methodology....Pages 101-104
Front Matter....Pages 105-105
Electroporation and Electrostimulation of Blind Retina Using Micro-electrode Arrays....Pages 107-110
Front Matter....Pages 111-111
High Voltage Electric Discharges Assisted Extraction of Stilbenes from Grape Stems....Pages 113-116
Front Matter....Pages 117-117
Microbial Inactivation in a Non-commercial Juice of Mango and Papaya Submitted to Pulsed Electric Fields in Presence of a Stevia rebaudiana Bertoni Extract....Pages 119-123
Effects of Pulsed Electric Fields on Four Residual Fungicides in White Wines....Pages 124-127
Control of Polyphenoloxidase and Peroxidase Activities in Mango and Papaya Juice by Pulsed Electric Field and Stevia rebaudiana Bertoni Extract Combined Process....Pages 128-131
Antimicrobial Capacity of a Cauliflower By-Product Infusion Combined with the PEF Treatment against S. Typhimurium....Pages 132-135
Front Matter....Pages 136-139
Study of Transmembrane Voltage Kinetics during 100 μs Pulse Using Voltage Sensitive Dyes....Pages 140-143
Electroporation of a Bladder Cancer Cell Line in Presence of Calcium: Efficacy Dependence on Electric Field Strength and Calcium Concentration....Pages 145-145
Extremely Low Frequency Electromagnetic Stimulation Alters Osteoblast Actin Filament Morphology....Pages 147-150
The Effect of Pulsed Electric Field on Mesenchymal Stem Cell Direct Migration....Pages 151-154
Cell Sensitization is Induced by a Wide Range of Permeabilizing Electric Fields....Pages 155-158
Effect of Electrode Distance in Electrochemotherapy: From Numerical Model to in Vitro Tests....Pages 159-162
Numerical Analysis of Split Dose Protocols for Nspef-electroporation....Pages 163-166
The Effect of Temperature on Protein Extraction by Electroporation and on Bacterial Viability....Pages 167-170
Effects of the Content of Cholesterol on the Permeability of Vesicles Membranes Induced by Pulsed Electric Fields....Pages 171-174
Front Matter....Pages 175-178
Electrostransfer of Plasmid gWIZ Blank into B16-F10 and TS/A Increase Expression of Cytosolic DNA PRRs....Pages 179-182
Glucose Derivatives as Efficient Markers of Cell Reversible Electropermeabilization....Pages 145-145
Effects of Nanosecond Pulsed Electric Fields on Cell-Cell Communication in a Monolayer....Pages 183-186
Spheroids, A Three-Dimensional Preclinical Model for Electrochemotherapy and Gene Electrotransfer....Pages 187-191
Mixed Spheroids as a Relevant 3D Biological Tool to Understand Therapeutic Window of Electrochemotherapy....Pages 192-195
Front Matter....Pages 196-199
Modeling of In Vivo Tissue Electroporation and Cellular Uptake Enhancement....Pages 200-203
Dynamic Modeling of Electroporation for the Computation of the Electric Field Distribution Inside Biological Tissues during the Application of the Pulse Voltage....Pages 205-205
Effects of Pulse Addition in Electropermeabilization: Theoretical Insights on the Electric Conductivity....Pages 207-210
Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP....Pages 211-214
Incorporation of the Blood Vessel Wall into Electroporation Simulations....Pages 215-218
Design of an Applicator for nsPEF Exposure of Newborn Mice....Pages 219-222
Ex vivo Evaluation of Transdermal Drug Delivery by Means of Electroporation with in vivo Oriented Experimental Protocols....Pages 223-227
Front Matter....Pages 228-231
Sensitivity of Cells to Nanosecond Pulsed Electric Fields is Dependent on Membrane Lipid Microdomains....Pages 232-235
Phase Transition to the Gel State Creates Long-Lived Electropores in Biomembranes....Pages 237-237
Front Matter....Pages 239-242
Safety of Clinical Irreversible Electroporation....Pages 243-246
Percutaneous and Intraoperative Irreversible Electroporation of Liver Cancer – A Monocenter Experience....Pages 247-247
Web-Based Tool for Patient-Specific Planning of Electroporation-Based Tumor Treatments in Different Tissue....Pages 249-251
Front Matter....Pages 252-255
Electrochemotherapy of Colorectal Liver Metastases – Trial Update....Pages 256-259
Treatment of Primary Liver Tumors with Electrochemotherapy – Clinical Trial....Pages 261-261
Technical Approach for Coupling Treatment Planning with Navigation Systems for Electroporation-Based Treatments....Pages 263-266
Front Matter....Pages 267-270
Proper Patient and Treatment Parameters Selection for Electrochemotherapy of Deep Seated Head and Neck Tumors....Pages 271-274
Gemcitabine + Cisplatin Combination Electrochemotherapy for Triple Negative Breast Cancers: An in vitro Model Study....Pages 261-261
Prototype of a Flexible Grid Electrode to Treat Large Surfaces by Means of Electrochemotherapy....Pages 275-279
Histological Characteristics of Soft Tissue Sarcomas Correlated to Electrical Resistance....Pages 280-284
3D Assessment of Irreversible Electroporation Treatments in Vegetal Models....Pages 285-289
Modeling of Tumor Growth Inhibition in Mice Following Electrochemotherapy....Pages 290-293
A Novel Sample Preparation Concept for Sepsis Diagnostics Using High Frequency Electric Fields....Pages 294-297
Cryopreservation of Human Umbilical Stem Cells in Combination with Trehalose and Reversible Electroporation....Pages 298-301
Front Matter....Pages 302-306
Constructing Clinically Applicable Plasmids for Cancer Gene Therapy....Pages 307-310
Evaluation of Smooth Muscle γ Actin Promoter Suitability for Tissue-Specific Gene Delivery of Interleukin 12....Pages 311-311
Utilization of Multi-array Electrodes for Delivery of Drugs and Genes in the Mouse Skin....Pages 313-316
Melanoma Cell Viability Is Reduced after Endoglin Silencing with Gene Electrotransfer....Pages 317-320
Front Matter....Pages 321-324
Custom Experimental Apparatus Design for the Investigation of Ultrasound as an Active Enhancement Method in Transdermal Drug Delivery....Pages 325-328
Magnetofection: An Effective, Selective and Feasible Non-viral Gene Delivery Method....Pages 329-329
Emulating Exposures of Biological Samples to Lightning Strokes....Pages 331-334
Mouse Melanoma (B16-F1) Cell Viability after Sonoporation in Acidic and CO2 Saturated Media....Pages 335-338
Front Matter....Pages 339-342
Antibacterial Efficacy of a Novel Plasma Reactor without an Applied Gas Flow....Pages 343-346
Front Matter....Pages 347-347
Effect of Pulsed Electric Fields on Water Distribution in Apple Tissue as Monitored by NMR Relaxometry....Pages 349-352
Pulsed Electric Field Effects on Wine Yeast in Defined Grape Juice Medium....Pages 353-353
Improving the Extraction of Juice and Anthocyanin Compounds from Blueberry Fruits and Their by-Products by Pulsed Electric Fields....Pages 355-358
Front Matter....Pages 359-362
Electroeradication of Escherichia Coli is Under the Control of the Conductance of the Pulsing Buffer....Pages 363-366
Front Matter....Pages 353-353
Comparison of the Efficacy of Pulsed Electric Fields Treatments in the Millisecond and Microsecond Range for the Extraction of Betanine from Red Beetroot....Pages 367-371
Extraction of Non-polar Molecules from Green Alga Chlorella vulgaris by Electroporation....Pages 373-373
Extraction of Sugar Solution from Sugar Beet Cossettes by Electroporation and Compressive Load....Pages 375-378
Front Matter....Pages 379-383
Animal Models for Translational Cancer Immunotherapy Studies....Pages 384-387
Gene Electro Transfer to Left Ventricular Myocardium in Rat and Porcine Models....Pages 389-389
Methodology for Assessing the Degree of Degeneration of the Porcine Intervertebral Discs Based on Magnetic Resonance Imaging....Pages 391-394
Front Matter....Pages 395-398
The Use of Pulsed Electric Fields for Protein Extraction from Nanochloropsis and Chlorella....Pages 399-402
A Microfluidic Device for the Real-Time Characterization of Lipid Producing Algae Cell Population Submitted to a Pulsed Electric Field....Pages 403-403
Design, Optimization and Scale-Up of Pulsed Electric Field Technologies for Large Flow Applications in Heterogeneous Matrices....Pages 405-408
Electro-biorefinery as a Potential Tool for Valorization of Mango and Papaya By-products....Pages 409-413
Front Matter....Pages 414-417
Electrochemotherapy in Veterinary Medicine – Our Clinical Experiences....Pages 418-421
Electrochemogene Therapy as an Effective and Safe Treatment of Canine Cutaneous Mast Cell Tumors....Pages 423-423
Numerical Study for Needle Electrode in Treatment of Cutaneous Tumor Model....Pages 425-428
Benefits and Side-Effects of Electrochemotherapy in Veterinary Patients....Pages 429-432
Electrochemotherapy in Non-satisfactory Responding Tumors in Vet Patients: Combined Administration of Bleomycin, Systemic and Local....Pages 433-436
Back Matter....Pages 437-440
....Pages 441-444