13th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft fur forensische Hamogenetik e.V.) New Orleans, October 19–21, 1989

Front Matter....Pages I-XVIII
Front Matter....Pages 1-1
Measurement Error in Determination of Band Size for Highly Polymorphic Single-Locus DNA Markers....Pages 3-11
Use of a Spreadsheet Template and Microcomputer to Calculate Gene Frequencies of Multi-allele Systems....Pages 12-13
Likelihood Calculations in Paternity Testing on the Basis of DNA-Fingerprints....Pages 14-16
Biostatistical approaches using minisatellite DNA patterns in paternity cases (mother-child-putative-father trios)....Pages 17-19
Kinship plausibilities from DNA fingerprints....Pages 20-22
Rapid Preparation of DNA for RFLP Analysis and DNA Fingerprinting....Pages 23-25
A Simple and Efficient Non-Organic Procedure for the Extraction of DNA From Evidentiary Samples....Pages 26-28
DNA Extraction from small samples using a microextraction method with low gelling temperature (LGT) agarose....Pages 29-33
VNTR Polymorphism: Reproducibility in techniques and interpretation....Pages 34-36
A Non-Isotopic DNA Detection System With the Sensitivity of 32 P: Applications For Paternity and Forensic Identifications....Pages 37-39
Rapid, Simple, Non-isotopic Probing of Southern Blots for DNA Fingerprinting....Pages 40-42
Simultaneous DNA Probing of Paternity Cases Using Non-isotopic Methods....Pages 43-47
Comparison of Chemiluminescent and Radioactive Methods of DNA Typing....Pages 48-50
On the effectiveness of three DNA probes in hybridization experiments over HINF I blots containing aged/degraded samples....Pages 51-53
Accuracy, Precision, and Site-to-Site Reproducibility in Analysis Of DNA Polymorphisms for Identity Testing....Pages 54-56
Elements of a Quality Assurance (QA)/Quality Control (QC) Program for DNA Testing....Pages 57-59
Investigation of Aged Blood Samples by Means of Single-Locus DNA Probes....Pages 60-62
Problems Associated with the Determination of Band Match Probabilities....Pages 63-67
Allele frequencies distribution of two VNTR markers (YNH24; YNZ22) in PST I digests from random Italian individuals (population of Rome)....Pages 68-70
Hardy-Weinberg equilibrium analysis of DNA polymorphic loci that exhibit a continuous distribution of fragment sizes....Pages 71-74
Front Matter....Pages 1-1
Segregation of Single-Locus DNA-Fragments in a Large Family....Pages 75-76
Comparison of HLA Antigens and DNA Polymorphisms in Parentage Testing....Pages 77-79
A Comparative Paternity Study: DNA vs Traditional Testing....Pages 80-82
Compatibility of Several HVR-Type Probes with Pvu II Restriction Endonuclease and Advantages of Pvu II Over Other Commonly Used Enzymes....Pages 83-87
Analysis of Case Work Samples by Single Locus VNTR Polymorphisms....Pages 88-91
Routine Use of a Single Locus DNA Probe....Pages 92-94
Results on Selected Trios Tested with Two Single Locus DNA Probes....Pages 95-97
Paternity testing using the hypervariable 3’ HVR DNA probe....Pages 98-100
Analysis of Disputed Paternities Using Hae III/YNH24 and Hinf I/MS31 RFLPs....Pages 101-105
Investigation of the use of DNA Probes L336 and YNH24 in Problems of Doubtful Parentage....Pages 106-108
Clarification of a Two-Man Case Only by DNA Markers....Pages 109-111
Characterization of DNA Probe pH30 (D4S139) and Preparation of Related Synthetic Oligonucleotides....Pages 112-113
Casework experiences with DNA profiling....Pages 114-116
Exclusion of an individual charged with rape by allele-specific DNA profiling on fetal tissues....Pages 117-118
Rapid Sex Determination Using Restriction Enzymes Digestion....Pages 119-121
DNA fingerprinting with simple repetitive oligonucleotide probes in forensic medicine....Pages 122-124
Application of simple repeat oligonucleotides for DNA fingerprinting....Pages 125-127
DNA Fingerprinting with M13 Probe....Pages 128-129
Minisatellite DNA Probe MZ 1.3: Application in Paternity Testing and Estimate of the Number of Genetic Loci....Pages 130-132
Non-isotopic DNA fingerprint analyses with the minisatellite probe MZ1.3....Pages 133-135
Front Matter....Pages 1-1
DNA Fingerprinting in Paternity Analyses....Pages 136-139
A Review of the Cases Submitted for DNA Analysis in the FBI Laboratory....Pages 140-142
A Method for the Analysis of PCR Amplification Products to Identify Base-Pair Substitution and VNTR Polymorphisms....Pages 143-147
Analysis of PCR Products (pMCT118) by Polyacrylamide Gel Electrophoresis....Pages 148-150
Effects of UV Damage on DNA Amplification by the Polymerase Chain Reaction....Pages 151-153
Typing by polymerase chain reaction mediated amplification of DNA fragments in the HLA class I region and direct DNA sequencing....Pages 154-157
Use of the Polymerase Chain Reaction for Typing Gc Variants....Pages 158-161
Amplification of Y Chromosome-Specific Sequences in Biological Evidence....Pages 162-163
Front Matter....Pages 165-165
Differential Detection of A, B and H Antigens in Semen and Saliva Stains Detected by an Elisa Technique....Pages 167-170
A Modified ELISA for the Detection of ABH Antigens in Bloodstains....Pages 171-173
Mosaic Phenomenon of ABH and Lewis Antigen Expression in Minor Salivary Glands — an Immunohistochemical Demonstration of the Transferase Activity....Pages 174-176
Different Expression of Blood Group A Antigen in the Secretory Cells of Salivary Glands from German and Japanese Nonsecretor Individuals....Pages 177-179
Different Epitopes of ABH Antigens Exist in Red Cell and Body Fluid....Pages 180-181
Production of Monoclonal Antibody to ABH-Carrying α2-Seminoglycoprotein for ABO-Grouping of Semen by Enzyme-Linked Immunosorbent Assay (ELISA)....Pages 182-184
Isolation and Identification of ABO Blood Group of Seminal Stains Mixed with Vaginal Secretion or Blood....Pages 185-186
Histochemical Analysis of the Chemical Structure of Blood-group-related Carbohydrate Chains in Human Pancreas....Pages 187-189
Enzyme Immuno Assay for the Typing of the Gm(A) and Gm(F) Allotypes of Human IgG1 in Semen, Vaginal Secretions and Other Body Fluids....Pages 190-193
Production of Monoclonal Antibodies Against IgG Allotypes and Their Use in Dot Immunobinding....Pages 194-196
Allotyping By Immunoblot Using Polyclonal Antisera....Pages 197-199
Whose Heritage in the Giant Stone Culture in Easter Island?....Pages 200-202
Front Matter....Pages 165-165
The Generation of Class II Polymorphism by Recombination....Pages 203-206
Polymorphism of HLA-DRB3 and DRB4 Genes Detected by RFLPs....Pages 207-210
Human Red Cell Acid Phosphatase (ACP1): The Primary Structure of the two Isozymes Bf and Bs Encoded by the ACP1*B allele....Pages 211-213
Further Evidence for a Silent Allele and a Rare Variant in the ADA System....Pages 214-217
Intragenic recombination within the PGM 1 locus....Pages 218-221
Practical Value of PGM 1 Subtyping in Blood- and Semen Stains and Hair Roots....Pages 222-224
Additional Data on PGP ( EC 3.1.3.18) Polymorphism in Düsseldorf....Pages 225-227
Biochemical and population genetics of S-adenosylhomocysteine hydrolase in NW Portugal....Pages 228-231
Group Specific Component Phenotyping by Ultrathin-layer Agarose Isoelectric Focusing Using Chemical Spacers....Pages 232-235
Haptoglobin Subtypes in the Swiss Population: Phenotype and Gene Frequencies - Description of an Easy Method for Routine Typing....Pages 236-238
Haptoglobin Subtyping by Agarose Isoelectric Focusing....Pages 239-242
Haptoglobin Subtype Determination of Bloodstains....Pages 243-245
The Application of Agarose Isoelectric Focusing in Paternity Testing....Pages 246-250
Fast IEF of some polymorphic proteins and enzymes in bloodstains using the PhastSystem....Pages 251-254
Isoelectric Focusing in Miniaturized Gels: Application to GC, PI, Tf and ORM Subtyping in Central Spain....Pages 255-259
The Use of Hybrid Isoelectric Focusing for the Detection of Polymorphic Proteins and Enzymes in Bloodstains Analysis....Pages 260-262
Distribution of Twelve Human Salivary Polymorphisms in Japanese Population....Pages 263-264
Inheritance of Some Electrophoretic Phenotypes of Human Hair....Pages 265-265
Hp, GLO and Gc Polymorphisms in the Balearic Islands (Spain): The Mediterranean Blueprint....Pages 266-270
A Simple Dilution Technique for Assessing Red Cell Enzyme Activity in Cases of Single System Incompatibilities....Pages 271-273
Front Matter....Pages 165-165
A Rapid High Resolution Seperator IEF Technique for Phenotyping Alpha-1 Antitrypsin....Pages 274-276
PI M Subtyping: Indication for an Alpha 1 -Antitrypsin Null Allele in three Paternity Cases....Pages 277-280
Transferrin C subtypes in different ethnic groups and spontaneous abortion....Pages 281-285
Polymorphism of Plasminogen in West Germany....Pages 286-287
The Significance of Current and of Origin and Concentration of Sialidases for the Determination of Plasminogen Groups....Pages 288-290
Coagulation Factor XIIIA and XIIIB polymorphisms in the North of Germany (Mecklenburg area)....Pages 291-293
Polymorphisms of Coagulation Factors 13A and 13B in West Germany....Pages 294-295
Identification of F13A*7 as a Population Specific Marker....Pages 296-299
Factor XIIIB (FXIIIB) in Disputed Paternity Complete Analysis of a Family with the Rare Phenotypes 4-1 and 4-2....Pages 300-301
Study of Genetic Polymorphism of Coagulation Factor XIII B in a Italian Population Sample using PAGIEF and Semi-dry Electroblotting....Pages 302-304
Isoelectric focusing of Orosomucoid in agarose gels....Pages 305-307
ORMl Subtypes in Hanover and Lower Saxony, FRG, by PAG SIEF and Immunoblotting (N=1934); 11 Variants....Pages 308-312
A Silent Allele in the Orosomucoid (ORM) System....Pages 313-315
Genetic polymorphism of human peptidase C (PEPC)....Pages 316-319
Kallikrein Phenotyping by Isoelectric Focusing....Pages 320-324
Front Matter....Pages 325-325
The Probability of Paternity....Pages 327-333
Seroanalysis and Expert Opinion in Practice....Pages 334-336
The Effect of Blanks and Antigen Sharing in the HLA System on the Paternity Index....Pages 337-339
Paternity probability calculation in “exclusion” cases....Pages 340-343
Front Matter....Pages 345-345
A Remarkable Case of Affiliation: Exclusion from Paternity after 8 Years....Pages 347-349
Front Matter....Pages 345-345
Legal Aspects of Disputed Paternities in Singapore....Pages 350-351
Evaluation of unusual band patterns in stains of biological interest....Pages 352-354
Characterisation of Vaginal Secretion Stains in Forensic Cases — A Critical Review....Pages 355-357
Monoclonal Antibodies Detect Sperm Antigens in Seminal Stains....Pages 358-360
GDA-J/F3 Monoclonal Antibody: The Ideal Probe for Sperm Detection in Forensic Science....Pages 361-363
Preparation and Application of Monoclonal Anti-P30 Antibody....Pages 364-365
Chromosome polymorphisms in paternity investigations....Pages 366-368
Plasma Protein Polymorphism in HIV-Seropositive Patients: Gc- and Tf*c-Subtypes and Pi-System....Pages 369-372
Distribution of C3 and BF Phenotypes in HIV-seropositive Patients....Pages 373-376
Plasma Protein Polymorphism in HIV-Seropositive Patients: Allotype Frequencies in the Gm- and Km-System....Pages 377-378