Preeclampsia : Methods and Protocols

Front Matter ....Pages i-xv
 Diagnostic Imaging: Ultrasound  (Stefan C. Kane, Su Lynn Khong, Fabricio da Silva Costa)....Pages 1-8
 Biomarker Immunoassays in the Diagnosis of Preeclampsia: Calculating the sFlt1/PlGF Ratio Using the Cobas®e 411 Analyser  (Carin Black, Fabricio da Silva Costa)....Pages 9-26
 Assessing the Circulating Placental-Specific Anti-angiogenic Protein sFLT-1 e15a in Preeclampsia  (Kirsten Palmer)....Pages 27-37
 Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Preeclampsia  (Sebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, Euan M. Wallace)....Pages 39-52
 Genetic Approaches in Preeclampsia  (Hannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, Eric K. Moses)....Pages 53-72
 Epigenetics and Preeclampsia: Programming of Future Outcomes  (Alberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, Edward Araujo Júnior)....Pages 73-83
 Inflammatory and Immune System Markers  (Kelly J. McKelvey, Gaayathri Ariyakumar, Sharon A. McCracken)....Pages 85-101
 Methods to Enrich Exosomes from Conditioned Media and Biological Fluids  (Shayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, Carlos Salomon)....Pages 103-115
 Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants  (Mancy Tong, Lawrence W. Chamley)....Pages 117-129
 Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation  (Andrew Lai, Omar Elfeky, Gregory E. Rice, Carlos Salomon)....Pages 131-138
 Proteomics Method to Identification of Protein Profiles in Exosomes  (Andrew Lai, Vyjayanthi Kinhal, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, Carlos Salomon)....Pages 139-153
 Harvesting and Characterization of Syncytial Nuclear Aggregates Following Culture of First Trimester Human Placental Explants  (Priyadarshini Pantham, Lawrence W. Chamley)....Pages 155-163
 Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index  (Severine A. Degrelle, Thierry Fournier)....Pages 165-171
 Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of Off-Target Effects  (Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, Padma Murthi)....Pages 173-189
 Immunohistological Techniques  (Evangelina Capobianco, Nora Martinez)....Pages 191-201
 Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin  (Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, Carlos Salomon)....Pages 203-217
 Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta  (Hélène Clabault, Laetitia Laurent, J. Thomas Sanderson, Cathy Vaillancourt)....Pages 219-231
 Analyzing Trophoblast Function Using Cell-Based Assays  (Katie L. Powell, Anthony W. Ashton)....Pages 233-246
 Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis  (Gina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, Bill Kalionis)....Pages 247-266
 An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro  (Tejasvy Chollangi, Hélène Clabault, Andrée-Anne Hudon Thibeault, Hannah E. J. Yong, Shagun Narula, Ellen Menkhorst et al.)....Pages 267-276
 In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases  (Lucas Sagrillo-Fagundes, Laetitia Laurent, Josianne Bienvenue-Pariseault, Cathy Vaillancourt)....Pages 277-283
 Measurement of Oxidative Stress: Mitochondrial Function Using the Seahorse System  (Dilys T. H. Leung, Simon Chu)....Pages 285-293
 Co-culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-placental Interactions: Focus on Estrogen Biosynthesis  (Andrée-Anne Hudon Thibeault, J. Thomas Sanderson, Cathy Vaillancourt)....Pages 295-304
 Placental Lipid Transport  (Evemie Dubé, Guillaume Desparois, Julie Lafond)....Pages 305-316
 EG-VEGF Maintenance Over Early Gestation to Develop a Pregnancy-Induced Hypertensive Animal Model  (Déborah Reynaud, Frédéric Sergent, Roland Abi Nahed, Sophie Brouillet, Mohamed Benharouga, Nadia Alfaidy)....Pages 317-324
 Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Preeclampsia  (Bryan Leaw, Seshini Gurusinghe, Rebecca Lim, Euan M. Wallace)....Pages 325-334
 Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Preeclampsia  (Sebastian R. Hobson, Rebecca Lim, Euan M. Wallace)....Pages 335-345
 A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Preeclampsia in Moderate- and High-Risk Women: The MELPOP Trial  (Sebastian R. Hobson, Euan M. Wallace, John C. Kingdom, Ryan J. Hodges)....Pages 347-352
Back Matter ....Pages 353-355