Plant Senescence: Methods and Protocols

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
 Concepts and Types of Senescence in Plants  (Susheng Gan)....Pages 3-8
 Initiation, Progression, and Genetic Manipulation of Leaf Senescence  (Akhtar Ali, Xiaoming Gao, Yongfeng Guo)....Pages 9-31
Front Matter ....Pages 33-33
 Phenotypic Analysis and Molecular Markers of Leaf Senescence  (Liming Zhao, Yan Xia, Xiao-Yuan Wu, Jos H. M. Schippers, Hai-Chun Jing)....Pages 35-48
 Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose  (Chenxia Cheng, Junping Gao, Nan Ma)....Pages 49-63
 Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence  (Xiuying Xia)....Pages 65-80
 Quantitative Analysis of Floral Organ Abscission in Arabidopsis Via a Petal Breakstrength Assay  (Chun-Lin Shi, Melinka A. Butenko)....Pages 81-88
 Characterization of Climacteric and Non-Climacteric Fruit Ripening  (Xiaohong Kou, Mengshi Wu)....Pages 89-102
Front Matter ....Pages 103-103
 Ethylene Treatment in Studying Leaf Senescence in Arabidopsis  (Zhonghai Li, Hongwei Guo)....Pages 105-112
 The Assay of Abscisic Acid-Induced Stomatal Movement in Leaf Senescence  (Yanyan Zhang, Kewei Zhang)....Pages 113-118
 The EPR Method for Detecting Nitric Oxide in Plant Senescence  (Aizhen Sun)....Pages 119-124
 Hormone Treatments in Studying Leaf Senescence  (Zenglin Zhang, Yongfeng Guo)....Pages 125-132
Front Matter ....Pages 133-133
 Methods to Study Darkness-Induced Leaf Senescence  (Yi Song, Lin Li)....Pages 135-140
 Salt Treatments and Induction of Senescence  (Yasuhito Sakuraba, Dami Kim, Nam-Chon Paek)....Pages 141-149
 Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance  (Shoki Aoyama, Junji Yamaguchi, Takeo Sato)....Pages 151-159
 Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection  (Wei Liu, Wenwei Zhang, Na Zheng, Weibo Zhai, Fangjun Qi)....Pages 161-171
 Study of Hydrogen Peroxide as a Senescence-Inducing Signal  (Stefan Bieker, Maren Potschin, Ulrike Zentgraf)....Pages 173-193
 Identification of Postharvest Senescence Regulators Through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue  (Donald A. Hunter, Rubina Jibran, Paul Dijkwel, David Chagné, Kerry Sullivan, Aakansha Kanojia et al.)....Pages 195-220
Front Matter ....Pages 221-221
 Chlorophyll and Chlorophyll Catabolite Analysis by HPLC  (Aditi Das, Luzia Guyer, Stefan Hörtensteiner)....Pages 223-235
 Identification and Functional Analysis of Senescence-Associated Genes in Wheat  (Geng Wang, Ke Li, Chunjiang Zhou)....Pages 237-246
 Isolation, Purification, and Detection of Micro RNAs in Plant Senescence  (Yujun Ren, Ying Miao)....Pages 247-265
 In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay  (Branka Uzelac, Dušica Janošević, Snežana Budimir)....Pages 267-282
 Activities of Vacuolar Cysteine Proteases in Plant Senescence  (Dana E. Martínez, Lorenza Costa, Juan José Guiamét)....Pages 283-297
 Study of Autophagy in Plant Senescence  (Xuefei Cui, Jing Zheng, Jinxin Zheng, Qingqiu Gong)....Pages 299-306
 Isolation of Chloroplasts for In Organelle Protein Degradation Assay  (Scott W. Vande Wetering, Judy A. Brusslan)....Pages 307-311
 Plant Cell Walls: Isolation and Monosaccharide Composition Analysis  (Yingzhen Kong, Malcolm O’Neill, Gongke Zhou)....Pages 313-319
 Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope  (Chun-Lin Shi, Melinka A. Butenko)....Pages 321-328
Front Matter ....Pages 329-329
 RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco  (Wei Li, Yongfeng Guo)....Pages 331-337
 Comprehensive Metabolomics Studies of Plant Developmental Senescence  (Mutsumi Watanabe, Takayuki Tohge, Salma Balazadeh, Alexander Erban, Patrick Giavalisco, Joachim Kopka et al.)....Pages 339-358
Back Matter ....Pages 359-367