Noncanonical Amino Acids: Methods and Protocols

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
 Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates  (Robyn M. Barfield, David Rabuka)....Pages 3-16
 Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis  (Yoshihiko Iwane, Takayuki Katoh, Yuki Goto, Hiroaki Suga)....Pages 17-47
 Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains  (Eiko Seki, Tatsuo Yanagisawa, Shigeyuki Yokoyama)....Pages 49-65
 Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids  (Jonas Helma, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher)....Pages 67-93
Front Matter ....Pages 95-95
 Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids  (Moritz J. Schmidt, Daniel Summerer)....Pages 97-111
 Genetic Code Expansion in Enteric Bacterial Pathogens  (Huangtao Zheng, Shixian Lin, Peng R. Chen)....Pages 113-126
 Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins  (Ying Ma, Martino L. Di Salvo, Nediljko Budisa)....Pages 127-135
 Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering  (Mark B. van Eldijk, Jan C. M. van Hest)....Pages 137-145
 Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene  (Jeffery M. Tharp, Wenshe R. Liu)....Pages 147-154
 Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439  (Jingxuan He, Charles E. Melançon III)....Pages 155-168
 Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for Biophysical Investigations  (Michael Lammers)....Pages 169-190
 Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code  (Xiang Li, Chang C. Liu)....Pages 191-200
 Site-Specific Protein Labeling with Tetrazine Amino Acids  (Robert J. Blizzard, True E. Gibson, Ryan A. Mehl)....Pages 201-217
Front Matter ....Pages 219-219
 Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers  (Lisa Seidel, Irene Coin)....Pages 221-235
 Generation of Stable Amber Suppression Cell Lines  (Simon J. Elsässer)....Pages 237-245
 Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo  (Christian Hoffmann, Heinz Neumann, Petra Neumann-Staubitz)....Pages 247-262
 Genetically Encoding Unnatural Amino Acids in Neurons In Vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins  (Ji-Yong Kang, Daichi Kawaguchi, Lei Wang)....Pages 263-277
 Genetic Code Expansion- and Click Chemistry-Based Site-Specific Protein Labeling for Intracellular DNA-PAINT Imaging  (Ivana Nikić-Spiegel)....Pages 279-295
 MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells  (Christine Koehler, Edward A. Lemke)....Pages 297-311
 Production and Chemoselective Modification of Adeno-Associated Virus Site-Specifically Incorporating an Unnatural Amino Acid Residue into Its Capsid  (Rachel E. Kelemen, Sarah B. Erickson, Abhishek Chatterjee)....Pages 313-326
 Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis  (Simone Brand, Yao-Wen Wu)....Pages 327-335
 Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins  (Eszter Kozma, Giulia Paci, Gemma Estrada Girona, Edward A. Lemke, Péter Kele)....Pages 337-363
 Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction  (Mathis Baalmann, Marcel Best, Richard Wombacher)....Pages 365-387
 Genetic Encoding of Unnatural Amino Acids in C. elegans  (Lloyd Davis, Sebastian Greiss)....Pages 389-408
Back Matter ....Pages 409-411