MicroRNA Protocols

Front Matter ....Pages i-xi
 The MicroRNA  (Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin)....Pages 1-25
 Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro  (Mainak Bose, Suvendra N. Bhattacharyya)....Pages 27-39
 Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection  (Anna Majer, Stephanie A. Booth)....Pages 41-51
 Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid  (Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma)....Pages 53-63
 Profiling of MicroRNAs in the Biofluids of Livestock Species  (Jason Ioannidis, Judith Risse, F. Xavier Donadeu)....Pages 65-77
 Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders  (Gabriel R. Fries, Joao Quevedo)....Pages 79-85
 Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation  (Max Hübner, Pedro A. F. Galante, Simone Kreth, Ludwig Christian Hinske)....Pages 87-92
 How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells  (Anna E. Zielak-Steciwko, John A. Browne)....Pages 93-105
 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs  (Shi-Lung Lin, Shao-Yao Ying)....Pages 107-126
 Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration  (Sang-Ging Ong, Won Hee Lee, Yang Zhou, Joseph C. Wu)....Pages 127-136
 Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime  (Nuno Bastos, Sónia A. Melo)....Pages 137-143
 Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets  (Yoichi Robertus Fujii)....Pages 145-157
 In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis  (Jian Xu, Xuelian Xiao, Daheng Yang)....Pages 159-171
 Isolation and Identification of Gene-Specific MicroRNAs  (Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying)....Pages 173-180
 Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells  (Masashi Fukuoka, Hirohiko Hohjoh)....Pages 181-192
 Screening miRNA for Functional Significance by 3D Cell Culture System  (Bo Han)....Pages 193-201
 Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation  (Lichan Tao, Yihua Bei, Yongqin Li, Junjie Xiao)....Pages 203-213
 Gene Manipulation with Micro RNAs at Single-Human Cancer Cell  (Andres Stucky, Xuelian Chen, Jiang F. Zhong)....Pages 215-223
 Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis  (Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro et al.)....Pages 225-237
 Transgene-Like Animal Models Using Intronic MicroRNAs  (Shi-Lung Lin, Shin-Ju E. Chang, Shao-Yao Ying)....Pages 239-254
 Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming  (Zhonghui Zhang, Wen-Shu Wu)....Pages 255-263
 Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction  (Shi-Lung Lin, Shao-Yao Ying)....Pages 265-282
 The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms  (Shao-Yao Ying, William Fang, Shi-Lung Lin)....Pages 283-304
 Identification and Isolation of Novel Sugar-Like RNA Protecting Materials: Glycylglycerins from Pluripotent Stem Cells  (Shi-Lung Lin)....Pages 305-316
Back Matter ....Pages 317-319